FastQCFastQC Report
Thu 26 May 2016
SRR1926523_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926523_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660138
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC60850.9217769617867778No Hit
GTACATGGGAAGCAGTGGTATCAAC58540.8867842784387507No Hit
GAGTACATGGGAAGCAGTGGTATCA26150.39612929417788406No Hit
GTCCTACAGTGGACATTTCTAAATT23130.35038128391336354No Hit
CATGTACTCTGCGTTGATACCACTG22080.3344755187551694No Hit
CTGTAGGACGTGGAATATGGCAAGA21900.3317488161566218No Hit
GCGTTGATACCACTGCTTCCCATGT18030.2731247102878489No Hit
ACGCAGAGTACATGGGAAGCAGTGG16490.24979625472249745No Hit
GTATCAACGCAGAGTACATGGGAAG16280.2466151016908586No Hit
GTCCTACAGTGTGCATTTCTCATTT15950.24161614692685468No Hit
CTTTAGGACGTGAAATATGGCGAGG15510.23495087390818284No Hit
GTCCTAAAGTGTGTATTTCTCATTT14220.21540950528525857No Hit
ACTCTGCGTTGATACCACTGCTTCC13720.20783533140040417No Hit
CTGTAGGACCTGGAATATGGCGAGA13320.20177599229252063No Hit
GTATCAACGCAGAGTACTTTTTTTT12380.18753654538899442No Hit
GATATACACTGTTCTACAAATCCCG10890.16496550721212835No Hit
GCTTCCCATGTACTCTGCGTTGATA10470.1586032011488507No Hit
TATCAACGCAGAGTACATGGGAAGC10270.15557353159490894No Hit
GGTATCAACGCAGAGTACATGGGAA9610.14557562206690117No Hit
GGTATCAACGCAGAGTACTTTTTTT8850.1340628777619225No Hit
GTGTATATCAATGAGTTACAATGAA8690.13163914211876912No Hit
CTGAAGGACCTGGAATATGGCGAGA8660.13118469168567784No Hit
CAGTGGTATCAACGCAGAGTACATG7730.1170967282598487No Hit
TATCAACGCAGAGTACTTTTTTTTT6680.10119096310165451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1500.016.41920518
TAGGACC5100.015.5232214
CCAACGT508.918716E-515.15618819
GTCTTAG456.5820373E-414.8284081
CTAGGAC801.2216515E-714.3086393
AGGACCG400.0051356214.3075475
ATAGGAC954.6075E-914.057613
GTCTAGA706.995677E-613.6179271
GTAGGAC12000.013.2752373
CCAACGA1301.4551915E-1113.11593219
CTGTAGG12150.012.9454361
GATATAC4500.012.9218991
TGTAGGA12850.012.8444722
GGACCTG7600.012.8014896
TAGGACT751.415603E-512.717824
ACTGTTC4200.012.6704668
TAGGACA3550.012.6282584
GTATGGA701.05482344E-412.2561341
TAGCCTC550.002974046412.1397375
GTATTAA550.002987670512.1323351
Produced by FastQC (version 0.11.5)