FastQCFastQC Report
Thu 26 May 2016
SRR1926522_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926522_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596394
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC19900.33367203560062647No Hit
CCCATGTACTCTGCGTTGATACCAC19060.3195873868617055No Hit
GAGTACATGGGAAGCAGTGGTATCA9530.15979369343085276No Hit
GCGTTGATACCACTGCTTCCCATGT6880.11535998014735224No Hit
CATGTACTCTGCGTTGATACCACTG6740.11301253869086543No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAG300.00764655918.231173
GCTAGAC400.00206091816.4080523
CCTGAAC555.8813555E-515.9108393
TATAGGA706.723887E-515.6617472
GTATAGA604.4940712E-415.5889831
TGTAGGA2850.014.90613652
CTGTAGG2600.014.9037511
GTAGGAC2850.014.5849343
GTCCTAA1201.7860657E-814.4754841
GTCCTAC2900.014.2835011
TTTAGGA1551.2551027E-1014.1460952
AGGACGT2800.014.1393535
GGACGTG2600.014.0151816
TAGGACC1158.4946805E-1014.0037444
TAGGACG2850.013.93823054
GACGTGG1650.013.5816267
AACAGCC450.00724662713.5816267
GTCTTAG700.001443833213.3619861
GCCTTAG600.00806643413.3619851
CTATTAC600.00806643413.3619851
Produced by FastQC (version 0.11.5)