FastQCFastQC Report
Thu 26 May 2016
SRR1926522_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926522_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596394
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC54130.9076214717116537No Hit
GTACATGGGAAGCAGTGGTATCAAC50710.8502768304174757No Hit
GAGTACATGGGAAGCAGTGGTATCA23190.3888369098280667No Hit
CATGTACTCTGCGTTGATACCACTG19380.3249529673336754No Hit
GCGTTGATACCACTGCTTCCCATGT16740.28068692843992393No Hit
ACGCAGAGTACATGGGAAGCAGTGG15510.26006297850078974No Hit
GTATCAACGCAGAGTACATGGGAAG14180.237762284664165No Hit
GTATCAACGCAGAGTACTTTTTTTT13680.22937856517671204No Hit
ACTCTGCGTTGATACCACTGCTTCC11910.19970019819112866No Hit
GCTTCCCATGTACTCTGCGTTGATA10210.17119555193378874No Hit
TATCAACGCAGAGTACATGGGAAGC9490.15912299587185652No Hit
CTGTAGGACGTGGAATATGGCAAGA9430.15811694953336217No Hit
GGTATCAACGCAGAGTACATGGGAA8970.15040392760490548No Hit
GGTATCAACGCAGAGTACTTTTTTT8750.1467150910304262No Hit
GTCCTACAGTGGACATTTCTAAATT8610.14436764957393938No Hit
CAGTGGTATCAACGCAGAGTACATG7780.13045067522476753No Hit
TATCAACGCAGAGTACTTTTTTTTT6640.11133579479337485No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6210.10412579603416533No Hit
GTGGTATCAACGCAGAGTACATGGG6050.1014430057981804No Hit
CATGGGAAGCAGTGGTATCAACGCA6020.10093998262893321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2000.014.7832884
TCCTATA653.2200332E-614.6769542
CCTATAC852.560555E-713.4659913
GTAGGAC4700.013.3943633
TCCAACG1001.0422809E-813.26308418
GGTTTCC655.550203E-513.1261414
CCAACGA655.5852583E-513.11733519
CTATACT1102.575689E-913.0058254
GTCCTAC5350.012.6573821
TGTAGGA5400.012.543362
TACAAGG701.04680264E-412.265742
GTCTTAT701.0492819E-412.2626341
ATAGGGT550.002975239212.138774
GATATAC1650.012.138771
TCCTACA6150.012.0995372
TAGGACG4700.011.9727374
TTGAACA657.749415E-411.7385894
CTAAATT3400.011.70272119
CCTACAG6450.011.3869393
CTGTAGG5650.011.1412721
Produced by FastQC (version 0.11.5)