FastQCFastQC Report
Thu 26 May 2016
SRR1926521_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926521_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388724
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT7050.18136261203321638No Hit
CTGTAGGACGTGGAATATGGCAAGA5190.1335137526882827No Hit
GTACATGGGAAGCAGTGGTATCAAC4590.11807863677056214No Hit
GTCCTACAGTGTGCATTTCTCATTT4230.10881756721992983No Hit
GTCCTAAAGTGTGTATTTCTCATTT4040.10392978051265164No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAC454.8382244E-520.8514881
GTCCTAG405.2551035E-420.1067921
TACTAAC350.00502306419.6020582
TAGGACC1950.018.178434
GTGTAGA450.001160271817.8727041
ATATTAT552.255593E-417.0603081
GTGTAGG808.044026E-716.755661
TCCTACA4450.016.3423922
CCCCTGT500.002348446316.0854341
GTCCTAC4000.016.0854341
ATCTACA706.63376E-515.6816472
ACTGTTC1400.015.3191418
TGTAGGA4150.014.8786732
TGTAGAA1507.2759576E-1114.6362052
CTGTAGG3850.014.2749521
GTAGATC550.001031880813.96830753
GTAGGAC4650.013.9253933
AGGACCT3300.013.8971075
GAACAGT901.7403181E-713.8921676
TTGACGA600.0067565713.7214422
Produced by FastQC (version 0.11.5)