##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926521_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 388724 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.158400304586287 27.0 7.0 31.0 2.0 31.0 2 16.37829925602741 16.0 2.0 30.0 2.0 31.0 3 20.024606147292165 24.0 15.0 31.0 2.0 31.0 4 21.944914643808975 28.0 2.0 35.0 2.0 37.0 5 22.3947916773855 28.0 2.0 35.0 2.0 37.0 6 22.9635628363569 30.0 2.0 35.0 2.0 37.0 7 23.15465471645692 30.0 7.0 35.0 2.0 37.0 8 23.541425278603842 31.0 8.0 35.0 2.0 37.0 9 26.196540476018974 32.0 17.0 37.0 2.0 39.0 10 26.924460542698675 32.0 23.0 37.0 2.0 39.0 11 24.036982537738858 30.0 7.0 37.0 2.0 39.0 12 25.16434796925325 31.0 11.0 37.0 2.0 39.0 13 21.813016433253413 27.0 2.0 36.0 2.0 39.0 14 25.144187135345387 31.0 17.0 38.0 2.0 40.0 15 26.255309679875698 31.0 17.0 38.0 2.0 40.0 16 26.61863944598224 31.0 17.0 39.0 2.0 40.0 17 25.94726849898643 31.0 11.0 38.0 2.0 40.0 18 26.032200224323685 31.0 16.0 38.0 2.0 40.0 19 26.45008026260277 31.0 18.0 39.0 2.0 40.0 20 26.715494798365935 31.0 18.0 39.0 2.0 40.0 21 24.578055381195913 30.0 2.0 38.0 2.0 40.0 22 25.50866939010712 31.0 2.0 38.0 2.0 40.0 23 25.47718947119293 31.0 2.0 38.0 2.0 40.0 24 24.519407085747215 31.0 2.0 39.0 2.0 40.0 25 24.322748273839537 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.647675138391101 1101 2 10.419809912640872 1101 3 7.0227212534940655 1101 4 8.343949221517232 1101 5 8.080853468739985 1101 6 7.328378636555833 1101 7 7.113797504136727 1101 8 5.7464321240746905 1101 9 5.493081467583885 1101 10 5.2569217565812565 1101 11 7.831874214864026 1101 12 5.563155199395794 1101 13 9.953544104827746 1101 14 7.151165728639288 1101 15 6.567841495255266 1101 16 6.246947113687067 1101 17 2.152997028606574 1101 18 4.726712810582903 1101 19 5.281659822813108 1101 20 3.247396806284545 1101 21 0.035935648077771276 1101 22 4.2547519185820555 1101 23 5.261515779218296 1101 24 6.439617053765627 1101 25 6.936726366719384 1102 1 5.79357626896082 1102 2 10.78093161514586 1102 3 7.15384960640916 1102 4 7.946534014246115 1102 5 7.875413433271131 1102 6 6.909899363667339 1102 7 6.675513309944751 1102 8 6.896425473664582 1102 9 5.985836954172225 1102 10 5.381226788724906 1102 11 8.062399597262605 1102 12 5.897883640983025 1102 13 10.127674009505203 1102 14 7.621105874948309 1102 15 5.504077363133785 1102 16 5.66081100196018 1102 17 6.537018309918924 1102 18 6.401583127585788 1102 19 5.248988574752811 1102 20 5.722737085601771 1102 21 4.848864045328934 1102 22 5.791258236471659 1102 23 6.230156878752766 1102 24 7.465846271234035 1102 25 7.102268375143236 1103 1 4.186444882948951 1103 2 8.404373951931618 1103 3 5.633803056892113 1103 4 7.100394132947386 1103 5 5.996281581764091 1103 6 5.773902622133534 1103 7 5.930115893442949 1103 8 5.663030850133804 1103 9 4.421172692563939 1103 10 3.558508296929258 1103 11 4.645990892502919 1103 12 3.410628898750975 1103 13 7.492331288685932 1103 14 5.706908272248441 1103 15 4.52157067164071 1103 16 4.231075170469431 1103 17 2.014132239861901 1103 18 0.9774681502786748 1103 19 3.165657956807973 1103 20 3.2050040470820456 1103 21 5.192974367684339 1103 22 0.9034611923659916 1103 23 3.416527180160891 1103 24 5.12764541006797 1103 25 1.5452009947173053 1104 1 5.448580741232735 1104 2 10.311669540011692 1104 3 6.885280733421684 1104 4 7.204020597430372 1104 5 7.007676402859683 1104 6 6.6395549987299525 1104 7 6.704068766116489 1104 8 6.717592736634174 1104 9 5.644235880826965 1104 10 4.553525536488767 1104 11 7.598557915688367 1104 12 4.7481922277451005 1104 13 8.952919984460479 1104 14 6.663413567256004 1104 15 5.865387738804625 1104 16 5.475014937559465 1104 17 6.158073766090659 1104 18 3.516815685725323 1104 19 4.4667345497080895 1104 20 3.9097853861384415 1104 21 3.0241234370999557 1104 22 4.999809220371482 1104 23 5.2218652866239665 1104 24 6.212684374990744 1104 25 5.4826306291682805 1105 1 3.2456578473248214 1105 2 9.088397808894477 1105 3 6.100531051764957 1105 4 5.550001094716077 1105 5 2.0131301258005045 1105 6 3.995525610619236 1105 7 5.396218253416265 1105 8 3.1441266485703707 1105 9 3.510372533866306 1105 10 3.118593226321888 1105 11 6.140231563567195 1105 12 3.658256063949107 1105 13 4.459307793588554 1105 14 3.1137017516940446 1105 15 3.758043224361895 1105 16 3.977494664119348 1105 17 4.65011518422617 1105 18 2.0091335598624447 1105 19 2.146213136934147 1105 20 1.5716042990799366 1105 21 2.5690768584524086 1105 22 3.4233398148356606 1105 23 0.7110835025612339 1105 24 3.7393288693275863 1105 25 4.428080833578171 1106 1 6.427236336038611 1106 2 11.274195706890964 1106 3 7.7881934341707115 1106 4 9.160546780663946 1106 5 8.785466446362118 1106 6 7.70308123102642 1106 7 7.5882905467726225 1106 8 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5.831105176820305 2204 5 1.0008201596763442 2204 6 3.6785924859761963 2204 7 4.759162792081376 2204 8 5.074528580844845 2204 9 3.6042286683581892 2204 10 3.0683418824058073 2204 11 4.543116459825157 2204 12 4.533170502381836 2204 13 2.705644450561877 2204 14 3.6932925385116775 2204 15 4.32338610280561 2204 16 4.094917833101807 2204 17 -1.6545515725435465 2204 18 2.17716557841786 2204 19 3.3835096965961746 2204 20 3.909996482785523 2204 21 5.377543202782675 2204 22 4.563067848946929 2204 23 3.539068783791752 2204 24 5.047463866951482 2204 25 5.216910525730924 2205 1 6.062711735264429 2205 2 10.623740782379809 2205 3 7.722095578725931 2205 4 8.11328690737069 2205 5 8.213711595286107 2205 6 7.671896186903101 2205 7 7.886586602433972 2205 8 7.78491197241657 2205 9 6.432095179722484 2205 10 5.75815440388261 2205 11 8.016154986416225 2205 12 7.102213116816927 2205 13 10.230124656203635 2205 14 7.774770499409396 2205 15 6.878409108085002 2205 16 6.137942837676089 2205 17 6.649035555210503 2205 18 6.806715870948619 2205 19 5.407840401392249 2205 20 5.8702024815627425 2205 21 -0.48570368782286977 2205 22 4.3642875080850025 2205 23 5.780081997881425 2205 24 7.593974249450511 2205 25 7.779232296177952 2206 1 3.4868040003267744 2206 2 8.250559476706023 2206 3 5.834283587097019 2206 4 0.9252993391891557 2206 5 4.883201216714767 2206 6 5.431025543552334 2206 7 5.5280900702789175 2206 8 5.549822457498802 2206 9 4.131324639970206 2206 10 3.6036657601906725 2206 11 2.787169045022182 2206 12 4.070540687744444 2206 13 7.309783155648198 2206 14 -0.2226936259738963 2206 15 1.6943865743738833 2206 16 2.398178909088312 2206 17 3.4199476795619717 2206 18 -1.2535085508345247 2206 19 0.3164573823908725 2206 20 1.752603636540254 2206 21 2.4164955957249425 2206 22 -2.742425174616546 2206 23 -3.562604323373737 2206 24 0.1333042293548985 2206 25 1.6666906383525237 2207 1 1.3591402614776023 2207 2 -13.846760692546447 2207 3 -6.291824433427969 2207 4 -4.568698735836421 2207 5 0.7565161074210138 2207 6 1.4980384634802597 2207 7 2.7040375753788872 2207 8 3.0533485505876605 2207 9 0.5376758448952366 2207 10 0.17053239013350918 2207 11 -1.730689431019556 2207 12 -2.8632938653446978 2207 13 0.21752764656913826 2207 14 -5.650964115147428 2207 15 -1.475687135600669 2207 16 -0.6652019836236214 2207 17 0.4134967956677933 2207 18 0.6461324838089624 2207 19 0.05689657519300084 2207 20 -0.26228767504530737 2207 21 -3.8992970639480546 2207 22 -4.189214967005835 2207 23 -1.6990474791211199 2207 24 0.13719655185470359 2207 25 0.5922078491170453 2208 1 4.972490930736633 2208 2 6.830658662292262 2208 3 6.776022440587607 2208 4 8.680589467908863 2208 5 3.6798648304013497 2208 6 6.0507495093141195 2208 7 6.698644176273376 2208 8 6.664849251849933 2208 9 5.159072470409143 2208 10 4.862998285110706 2208 11 7.2067948934110575 2208 12 6.666754069292445 2208 13 9.171206213626466 2208 14 3.573301365292469 2208 15 5.219425297107247 2208 16 5.562444403621633 2208 17 6.64099069097405 2208 18 0.9160010798999529 2208 19 4.06536790369876 2208 20 5.117489486076472 2208 21 7.570626910794466 2208 22 6.581449195582675 2208 23 3.0869742269025124 2208 24 4.67231083051767 2208 25 5.990709415169128 2209 1 2.8772159875630585 2209 2 4.938846503855352 2209 3 4.887015331239056 2209 4 0.1854499408981738 2209 5 4.513302950051202 2209 6 5.2165375830192 2209 7 5.703722282381612 2209 8 4.570589929898006 2209 9 4.330373916460314 2209 10 3.2258652387306697 2209 11 -0.7848383222391817 2209 12 2.924286593352992 2209 13 2.3367041098396513 2209 14 3.5858273603594526 2209 15 3.925717312389928 2209 16 3.6976187519313477 2209 17 2.0656170874532336 2209 18 3.0747765308841366 2209 19 3.8528612537979043 2209 20 3.815995071993189 2209 21 5.849922746747456 2209 22 4.53240919945171 2209 23 0.17417256321822805 2209 24 -2.91133283152228 2209 25 0.12806009466808632 2210 1 -1.779969151692839 2210 2 -13.846760692546447 2210 3 -7.511993564959051 2210 4 -1.6025550872824041 2210 5 -0.21045956287650114 2210 6 -0.4581856369540098 2210 7 -0.61749029388276 2210 8 -20.94665144941234 2210 9 -12.674240093174612 2210 10 -6.4784699926843174 2210 11 -9.354749650904413 2210 12 -4.101920012552455 2210 13 -4.844303124830574 2210 14 -3.5348901484144335 2210 15 -4.8790119869345645 2210 16 -4.370365834127803 2210 17 -4.012334243827816 2210 18 -3.9600646914772177 2210 19 -9.531518446497344 2210 20 -6.313117270058726 2210 21 -4.639484407510633 2210 22 -9.226681368350013 2210 23 -7.0639999498790225 2210 24 -9.99353486459298 2210 25 -7.263149793118227 2211 1 -0.10188736106474394 2211 2 4.834508189628782 2211 3 0.5731012912801816 2211 4 1.5678493756219005 2211 5 1.185318150909989 2211 6 0.12265325630038504 2211 7 -20.570640536209094 2211 8 -13.052391630681221 2211 9 -8.719452378851248 2211 10 -6.852335841997849 2211 11 -8.74533918963026 2211 12 -4.411221634406239 2211 13 -5.979925339785991 2211 14 -4.2890033511729975 2211 15 -4.85190218601797 2211 16 -11.511073729622437 2211 17 -7.469921034724351 2211 18 -5.382827748547143 2211 19 -8.178912889342005 2211 20 -6.543351689671294 2211 21 -4.16095700974892 2211 22 -5.881909321046351 2211 23 -7.850001150529128 2211 24 -22.03876911338135 2211 25 -21.826962394087534 2212 1 -0.5331488625851577 2212 2 -13.846760692546447 2212 3 -7.584967732603618 2212 4 -19.444235216523115 2212 5 -19.861509643651946 2212 6 -20.353177559409584 2212 7 -9.6178718065674 2212 8 -8.930364804461199 2212 9 -4.535816766699735 2212 10 -5.0994647672761 2212 11 -4.816803609953631 2212 12 -2.287974539924015 2212 13 -6.481947639204394 2212 14 -3.5670424588026286 2212 15 -3.403128850847672 2212 16 -3.3890862668947435 2212 17 -3.4975394450297124 2212 18 -2.758286579254605 2212 19 -3.396268253109877 2212 20 -3.4459073644107576 2212 21 -1.2643206201684336 2212 22 -2.4890173836736658 2212 23 -2.5708303424950394 2212 24 -1.5794857257591985 2212 25 -2.2162132084197808 2213 1 -18.689500654116102 2213 2 -13.846760692546447 2213 3 -8.850274779842884 2213 4 -6.159456165786617 2213 5 -19.861509643651946 2213 6 -10.61995333682366 2213 7 -20.570640536209094 2213 8 -10.994114624535088 2213 9 -8.597338168577366 2213 10 -7.5348779575926095 2213 11 -6.88260858604345 2213 12 -5.523491154434325 2213 13 -19.141556759725567 2213 14 -12.638959591500134 2213 15 -7.460505288257377 2213 16 -6.8129042189890825 2213 17 -5.350575798100717 2213 18 -5.423850587635162 2213 19 -6.372320754216091 2213 20 -6.306330017507886 2213 21 -4.454933008680886 2213 22 -5.34733423488677 2213 23 -4.954851304553287 2213 24 -4.05677238670377 2213 25 -4.841692344987699 2214 1 -18.689500654116102 2214 2 -13.846760692546447 2214 3 -12.04988082245401 2214 4 -19.444235216523115 2214 5 -19.861509643651946 2214 6 -20.353177559409584 2214 7 -16.315773279571925 2214 8 -14.647536405164551 2214 9 -13.325721929422055 2214 10 -13.347745301132141 2214 11 -13.694169497359585 2214 12 -11.70752139645741 2214 13 -19.141556759725567 2214 14 -16.190126255752844 2214 15 -23.717461098404677 2214 16 -17.745832973533354 2214 17 -23.429135536234924 2214 18 -17.287089438785895 2214 19 -14.180950159079726 2214 20 -12.7923343915936 2214 21 -10.369774484283047 2214 22 -11.48438505828668 2214 23 -11.384519772030078 2214 24 -10.226379732850376 2214 25 -9.965015491432666 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 70796.0 3 1.0 4 301.0 5 1127.0 6 1670.0 7 1684.0 8 1480.0 9 1428.0 10 1261.0 11 1163.0 12 1114.0 13 1219.0 14 1825.0 15 4020.0 16 5909.0 17 6442.0 18 6416.0 19 7380.0 20 8707.0 21 10173.0 22 10509.0 23 11353.0 24 12008.0 25 12344.0 26 10187.0 27 7966.0 28 8529.0 29 10083.0 30 9812.0 31 11308.0 32 14342.0 33 18152.0 34 22328.0 35 25883.0 36 25239.0 37 25885.0 38 18680.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.98729901042102 19.072374153963686 13.18742315543194 24.75290368018335 2 12.599023684392364 20.801375458791107 44.19596223178757 22.40363862502896 3 19.928456760918138 24.889991330578447 29.054048510473045 26.12750339803037 4 11.428674036786756 17.273494502337332 39.288984922577825 32.008846538298094 5 11.644469937323368 38.53656933565159 35.99425215245538 13.824708574569659 6 29.74790323215741 37.98171139872362 18.81987333068808 13.450512038430901 7 26.819393223055023 31.291801862722256 22.58564942407901 19.303155490143713 8 23.05931176626234 41.33246062361608 20.04651357110031 15.561714039021265 9 25.980129585183203 16.765726882229938 20.518598255029406 36.73554527755745 10 14.174143582912144 31.290992438193005 34.61139625697084 19.923467721924016 11 32.35948970995307 23.298394949616515 25.090537095591852 19.251578244838566 12 23.62046587881686 25.844934763303506 32.538561377143246 17.99603798073639 13 28.397910210519186 22.69627201764492 25.188986710861116 23.716831060974783 14 19.130105009873283 25.010396369770753 28.13401509108266 27.725483529273298 15 21.870156890219306 34.16422520858544 23.563520370252427 20.40209753094283 16 19.518052445202443 29.65324322385776 30.971413087657503 19.8572912432823 17 19.68817897476303 30.273752012882447 30.21167432026253 19.82639469209199 18 19.23120144048462 28.916840237397718 33.255019613274825 18.59693870884283 19 22.034110827536306 27.759129178628616 28.117269357463602 22.089490636371472 20 22.03385020985479 29.336848540819144 29.85123139747555 18.778069851850514 21 20.79451279494401 27.537899715667912 27.449770243501465 24.217817245886618 22 20.94504626347553 30.69870012321231 28.80081199789676 19.555441615415404 23 20.461627206287567 29.675372500141634 29.511590896214994 20.351409397355802 24 21.437255396509165 28.465115751827174 30.433279394219355 19.664349457444306 25 21.161215554983258 28.793973731650784 30.070563996909605 19.97424671645635 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 606.0 1 606.0 2 1162.5 3 1719.0 4 1719.0 5 1719.0 6 2301.0 7 2883.0 8 2883.0 9 2883.0 10 3425.0 11 3967.0 12 3967.0 13 3967.0 14 4883.5 15 5800.0 16 5800.0 17 5800.0 18 8200.0 19 10600.0 20 10600.0 21 10600.0 22 14613.0 23 18626.0 24 18626.0 25 18626.0 26 23894.0 27 29162.0 28 29162.0 29 29162.0 30 34879.0 31 40596.0 32 40596.0 33 40596.0 34 43832.0 35 47068.0 36 47068.0 37 47068.0 38 47888.0 39 48708.0 40 48708.0 41 48708.0 42 48254.5 43 47801.0 44 47801.0 45 47801.0 46 46838.5 47 45876.0 48 45876.0 49 45876.0 50 40677.0 51 35478.0 52 35478.0 53 35478.0 54 29032.0 55 22586.0 56 22586.0 57 22586.0 58 18154.0 59 13722.0 60 13722.0 61 13722.0 62 10647.0 63 7572.0 64 7572.0 65 7572.0 66 5635.5 67 3699.0 68 3699.0 69 3699.0 70 2575.0 71 1451.0 72 1451.0 73 1451.0 74 990.0 75 529.0 76 529.0 77 529.0 78 354.0 79 179.0 80 179.0 81 179.0 82 125.0 83 71.0 84 71.0 85 71.0 86 46.0 87 21.0 88 21.0 89 21.0 90 12.5 91 4.0 92 4.0 93 4.0 94 2.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.796935614986468 2 36.7090789351828 3 5.93505932229551 4 14.04132495035038 5 13.889031806628868 6 15.068017410810755 7 9.458124530515224 8 7.637552608020086 9 0.10495878824049967 10 1.5123841080046512 11 5.949722682417345 12 5.852224200203744 13 16.489334334900853 14 1.6410100739856555 15 3.0612979903479074 16 3.162397999608977 17 3.0296560027165804 18 1.562805486669205 19 1.5211306736913595 20 0.2765458268591597 21 4.547956905156358 22 1.6610757246786925 23 0.10341527664872764 24 9.224282524361758 25 0.10907481915189182 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 388724.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.18787317449375 #Duplication Level Percentage of deduplicated Percentage of total 1 97.54013364353106 88.9447733610948 2 1.557281843760408 2.840104385315317 3 0.3542987783390661 0.9692325619518247 4 0.14976439917418097 0.5462678815179788 5 0.09010194909373814 0.4108102553367242 6 0.05933045060576666 0.3246130563134529 7 0.039457715947064154 0.2518645638275259 8 0.02800669158461124 0.20430965122037528 9 0.020714797158782605 0.1700044466535388 >10 0.13922837491195647 2.436462028971288 >50 0.011563389827917481 0.8156100547255388 >100 0.009539796576026202 1.7632888310749537 >500 5.781694894561334E-4 0.3226589219966726 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 705 0.18136261203321638 No Hit CTGTAGGACGTGGAATATGGCAAGA 519 0.1335137526882827 No Hit GTACATGGGAAGCAGTGGTATCAAC 459 0.11807863677056214 No Hit GTCCTACAGTGTGCATTTCTCATTT 423 0.10881756721992983 No Hit GTCCTAAAGTGTGTATTTCTCATTT 404 0.10392978051265164 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 2.5725193196200904E-4 13 0.0 0.0 0.0 0.0 2.5725193196200904E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTAC 45 4.8382244E-5 20.851488 1 GTCCTAG 40 5.2551035E-4 20.106792 1 TACTAAC 35 0.005023064 19.602058 2 TAGGACC 195 0.0 18.17843 4 GTGTAGA 45 0.0011602718 17.872704 1 ATATTAT 55 2.255593E-4 17.060308 1 GTGTAGG 80 8.044026E-7 16.75566 1 TCCTACA 445 0.0 16.342392 2 CCCCTGT 50 0.0023484463 16.085434 1 GTCCTAC 400 0.0 16.085434 1 ATCTACA 70 6.63376E-5 15.681647 2 ACTGTTC 140 0.0 15.319141 8 TGTAGGA 415 0.0 14.878673 2 TGTAGAA 150 7.2759576E-11 14.636205 2 CTGTAGG 385 0.0 14.274952 1 GTAGATC 55 0.0010318808 13.9683075 3 GTAGGAC 465 0.0 13.925393 3 AGGACCT 330 0.0 13.897107 5 GAACAGT 90 1.7403181E-7 13.892167 6 TTGACGA 60 0.00675657 13.721442 2 >>END_MODULE