FastQCFastQC Report
Thu 26 May 2016
SRR1926521_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926521_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388724
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19100.49135119004743727No Hit
GTCCTACAGTGGACATTTCTAAATT15090.38819316533067166No Hit
CTGTAGGACGTGGAATATGGCAAGA14910.38356263055535544No Hit
GTACATGGGAAGCAGTGGTATCAAC12330.31719163210915713No Hit
GGTATCAACGCAGAGTACTTTTTTT11930.30690155483067677No Hit
CCCATGTACTCTGCGTTGATACCAC10460.26908552083226145No Hit
GTCCTACAGTGTGCATTTCTCATTT9480.24387483149998457No Hit
GTCCTAAAGTGTGTATTTCTCATTT9270.23847254092878237No Hit
TATCAACGCAGAGTACTTTTTTTTT9190.23641452547308628No Hit
CTTTAGGACGTGAAATATGGCGAGG9090.23384200615346623No Hit
CTGTAGGACCTGGAATATGGCGAGA8830.22715345592245398No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7640.1965404760189749No Hit
GATATACACTGTTCTACAAATCCCG6320.1625832209999897No Hit
GAGTACATGGGAAGCAGTGGTATCA6020.15486566304112945No Hit
CTGAAGGACCTGGAATATGGCGAGA5650.14534734155853513No Hit
GTGTATATCAATGAGTTACAATGAA4810.12373817927372635No Hit
GTGTATATCAATGAGTTACAATGAG4530.11653512517879008No Hit
GTTCTACAGTGTGGTTTTTATCATT4220.10856031528796782No Hit
ACGCAGAGTACATGGGAAGCAGTGG4110.10573054403638571No Hit
CACACACACACACACACACACACAC3930.10110000926106956No Hit
GTATCAACGCAGAGTACATGGGAAG3920.10084275732910752No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGAC350.002104305716.356573
GCCCTAG400.00514203114.3027321
GTCTATA551.8938717E-413.8693161
TCTACAC903.410787E-813.7819243
TAGGACC2500.013.7359594
GGACCTG3750.012.7168346
GTACAGA603.954495E-412.713541
CTGCTCA751.4706859E-512.6676129
CCAACGA905.5614873E-712.63013619
ACTGTTC2350.012.5328528
AGGACCT3900.012.4738945
TCCAAGG701.04237624E-412.2690162
GTAGGAC6850.012.2574783
GTTACAA2100.012.17905815
TATTACA550.002960443512.1450872
ATACTGG550.002971963312.1387956
CACTGTT2750.012.0933727
TGTAGGA7250.011.8459472
CTGTAGG7000.011.7146191
GATATAC2950.011.6361221
Produced by FastQC (version 0.11.5)