FastQCFastQC Report
Thu 26 May 2016
SRR1926520_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926520_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736847
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT13690.1857916229556475No Hit
CTGTAGGACGTGGAATATGGCAAGA9570.1298777086695067No Hit
GTCCTACAGTGTGCATTTCTCATTT8210.11142068841971264No Hit
GTCCTAAAGTGTGTATTTCTCATTT8120.11019926796200569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT752.0545485E-819.5342981
TAGGACC2900.016.9998444
CTATAGG552.3753272E-416.951251
TAGAGTG804.109461E-815.6370285
CTGTAGG7800.015.367891
TGTAGGA8250.014.8962192
TCCAACG1600.014.88828818
ACGTGTA400.004412453614.63798
GGACCTA507.4321864E-414.591836
ACTAGAC450.004511939414.5884213
GTATAAA658.4590196E-414.3433651
CTACACT1850.013.9035164
AGGACCG450.006219462513.8995795
GTCCTAC8400.013.6359551
CCCCTCA600.00824499413.3188391
GATATAC2300.013.3188391
GTCCCAG700.001480481613.3188391
GATCTAC802.670107E-413.3188391
GTAGGAC9200.013.2009343
TAGGACA2450.013.1232164
Produced by FastQC (version 0.11.5)