FastQCFastQC Report
Thu 26 May 2016
SRR1926520_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926520_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736847
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA27990.3798617623468644No Hit
GTCCTACAGTGGACATTTCTAAATT27370.3714475325271054No Hit
GTCCTACAGTGTGCATTTCTCATTT18910.2566340095026512No Hit
GTCCTAAAGTGTGTATTTCTCATTT16780.2277270586702531No Hit
GTATCAACGCAGAGTACTTTTTTTT16670.22623421144416683No Hit
CTTTAGGACGTGAAATATGGCGAGG16110.21863426192954574No Hit
CTGTAGGACCTGGAATATGGCGAGA15850.21510571394061456No Hit
GATATACACTGTTCTACAAATCCCG11060.15009900291376638No Hit
GGTATCAACGCAGAGTACTTTTTTT10860.1473847352299731No Hit
CTGAAGGACCTGGAATATGGCGAGA10020.1359848109580415No Hit
TATCAACGCAGAGTACTTTTTTTTT8270.1122349687248506No Hit
GTGTATATCAATGAGTTACAATGAG7410.10056361768453967No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG400.005136619314.3074455
TAGGACC5350.013.9063014
GATATAC3950.013.2793581
TAGGACT1301.2732926E-1113.2068724
TGTAGGA14350.012.8337712
ACTGTTC4150.012.5912198
CTGTAGG14400.012.5173031
GTAGGAC14300.012.4748613
TCCAACG2400.012.23669218
GACGTGG7250.012.1886967
ATCCCGT1405.4569682E-1112.18857310
GTTACAA4000.012.078815
CACTGTT4200.011.9905427
GTCCTAA5300.011.8762551
CCAACGA1850.011.7779519
AGGACCT9400.011.7706635
TTACAAT4200.011.72917916
TAGGACG12950.011.7111124
GGACCTG8900.011.6809356
AGGACGT12400.011.6151845
Produced by FastQC (version 0.11.5)