##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926520_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 736847 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.37688963923311 31.0 28.0 31.0 22.0 34.0 2 28.832393970525768 31.0 28.0 33.0 23.0 34.0 3 29.167000747780747 31.0 28.0 33.0 25.0 34.0 4 32.92790226464924 35.0 33.0 37.0 27.0 37.0 5 32.59792467092897 35.0 33.0 37.0 26.0 37.0 6 32.675966652507235 35.0 33.0 37.0 26.0 37.0 7 32.51167610100876 35.0 33.0 37.0 25.0 37.0 8 32.48734404835739 35.0 33.0 37.0 25.0 37.0 9 33.70699887493605 37.0 33.0 39.0 23.0 39.0 10 33.61130329634239 37.0 33.0 39.0 22.0 39.0 11 33.83521409464923 37.0 34.0 39.0 25.0 39.0 12 33.63672512746879 37.0 33.0 39.0 23.0 39.0 13 33.7774056215198 37.0 34.0 39.0 25.0 39.0 14 34.42093134667034 38.0 33.0 40.0 19.0 41.0 15 34.55392503464084 38.0 34.0 40.0 23.0 41.0 16 34.53082390238408 38.0 34.0 40.0 23.0 41.0 17 34.462846425377315 38.0 34.0 40.0 22.0 41.0 18 34.44032207500336 38.0 34.0 40.0 22.0 41.0 19 34.46112015113042 39.0 34.0 40.0 19.0 41.0 20 34.43651124317531 39.0 34.0 40.0 19.0 41.0 21 34.343145863388195 39.0 34.0 40.0 18.0 41.0 22 34.28884829550775 39.0 34.0 40.0 18.0 41.0 23 33.9292064702713 39.0 34.0 40.0 16.0 41.0 24 34.0062971010264 39.0 34.0 40.0 16.0 41.0 25 33.794604578698156 39.0 34.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.78065136437543 1101 2 0.7742535602612399 1101 3 0.7619295631687137 1101 4 0.5739072165499977 1101 5 0.6339677630905598 1101 6 0.1364158674732323 1101 7 0.5252914080538318 1101 8 0.6177962173433471 1101 9 1.1146643446477142 1101 10 0.9931519836827647 1101 11 0.7819662403276837 1101 12 0.8112895907514854 1101 13 0.7948928403573561 1101 14 1.2142042124018317 1101 15 0.9600062889626102 1101 16 0.7983782394492991 1101 17 0.6879464958736818 1101 18 0.8357044893088741 1101 19 0.7814566309842661 1101 20 0.9536807620373224 1101 21 1.0765869364899459 1101 22 0.9051898759639059 1101 23 1.0818712663392063 1101 24 0.8732062437904275 1101 25 0.9662916587717874 1102 1 0.6381920492899198 1102 2 0.7240304356890377 1102 3 0.6892715795230657 1102 4 0.568812620013297 1102 5 0.603928156043132 1102 6 0.11094592961196525 1102 7 0.4227283985028336 1102 8 0.5760005517086739 1102 9 0.745972302838652 1102 10 0.9995771675291323 1102 11 0.7196277681464025 1102 12 0.7924555384341332 1102 13 0.4869249059891061 1102 14 0.8706325628633067 1102 15 0.7409964895067844 1102 16 0.6581118053258521 1102 17 0.6722869752017786 1102 18 0.8776712739522523 1102 19 0.6807089444401839 1102 20 0.48640042160183583 1102 21 0.6337991459834527 1102 22 0.49191737411874215 1102 23 0.9542786002198014 1102 24 1.0513910669349684 1102 25 1.0336626131165048 1103 1 0.515637734683672 1103 2 0.46244013285630814 1103 3 0.456830454957597 1103 4 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1.0503401418880074 2202 20 0.9331208701784277 2202 21 0.9825424494092232 2202 22 0.9926863027530501 2202 23 1.0976927172323911 2202 24 0.9785650910604886 2202 25 1.0529523175991926 2203 1 0.12452665227102244 2203 2 0.10718756129490714 2203 3 0.04616969443405239 2203 4 -0.40234359256832875 2203 5 -0.24078950141210242 2203 6 -0.31421286137051396 2203 7 0.05690598171747041 2203 8 -0.009898962067666162 2203 9 0.29660306741993736 2203 10 0.45574562815241393 2203 11 0.2823726811604388 2203 12 0.20858801366069457 2203 13 0.08173469473111084 2203 14 0.557824847553448 2203 15 0.4289047841279725 2203 16 0.43111880475329656 2203 17 0.4997706625445346 2203 18 0.2878812057687199 2203 19 0.2967797012684201 2203 20 0.39903955897245424 2203 21 0.423371287012543 2203 22 0.5701346050284357 2203 23 0.8207430668720974 2203 24 0.7810332404937341 2203 25 0.6692110078643196 2204 1 0.0195699657286994 2204 2 -0.1408520103491604 2204 3 -0.1456023082160378 2204 4 -0.3392374505790414 2204 5 -0.2215158549342604 2204 6 -0.3720849559751471 2204 7 -0.13358527012442778 2204 8 -0.22384548370317958 2204 9 0.16537196208145843 2204 10 0.049562964265589926 2204 11 -0.09711197415975903 2204 12 -0.06529802010420838 2204 13 -0.15495903426875657 2204 14 0.1764006626362402 2204 15 0.08455374985775421 2204 16 0.16873457452009433 2204 17 -0.12475474847479262 2204 18 -0.17387718461510104 2204 19 0.09068175533569445 2204 20 0.20744348020347303 2204 21 0.234088420127037 2204 22 0.13774552129240902 2204 23 0.4755510914964489 2204 24 0.34062402407866443 2204 25 0.3512984632439071 2205 1 0.40359986465839626 2205 2 0.5512755744998721 2205 3 0.37836153493874747 2205 4 0.32541596945693385 2205 5 0.30000548548002115 2205 6 0.2585986964622151 2205 7 0.07611343465733711 2205 8 0.25034770992679967 2205 9 0.7488197045845553 2205 10 0.7333076384743933 2205 11 0.40515847137969985 2205 12 0.44428574337212723 2205 13 0.3113975746789279 2205 14 0.7949516217220989 2205 15 0.8428098348291755 2205 16 0.7425222807670124 2205 17 0.753999912146817 2205 18 0.7719052854167927 2205 19 0.8022855058279177 2205 20 0.696864623018044 2205 21 0.7736402909386797 2205 22 0.7713186704358534 2205 23 0.901039680733831 2205 24 1.0119770322416315 2205 25 0.9978078306055451 2206 1 0.162189512070249 2206 2 0.29833376761958164 2206 3 0.485713372898239 2206 4 0.26143211635079666 2206 5 0.5381257298268238 2206 6 0.34151942664475854 2206 7 0.6361177214433198 2206 8 0.6155354354296065 2206 9 0.8026224409829439 2206 10 0.7691701755372335 2206 11 0.7200443357029229 2206 12 0.7630475766875975 2206 13 0.689545683134611 2206 14 0.8383696190785841 2206 15 0.7444388135024127 2206 16 0.8530642019615939 2206 17 0.7656206744802532 2206 18 0.7329241115518954 2206 19 0.7305854379538061 2206 20 0.6937388415726815 2206 21 0.7848716702120697 2206 22 0.7053914743511172 2206 23 1.0205445569528848 2206 24 0.9644187140906624 2206 25 0.8999297896667358 2207 1 -1.253042683829193 2207 2 -0.06978548181894695 2207 3 0.20564020039950393 2207 4 0.3796926394254925 2207 5 0.2947583459247767 2207 6 0.36954232021862055 2207 7 0.4659952842919779 2207 8 0.4412653492164651 2207 9 0.7539663517269801 2207 10 0.6117297640324892 2207 11 0.5471398946393862 2207 12 0.4297251105570439 2207 13 0.45954133625350124 2207 14 -4.231517362012486 2207 15 -1.5682866809553104 2207 16 -0.5059990451585961 2207 17 -0.5668831290407184 2207 18 0.13761512075279114 2207 19 0.39555682098648504 2207 20 0.4464375302635517 2207 21 0.6309087635975672 2207 22 0.6007040875511436 2207 23 0.7832374498022645 2207 24 0.4305709998256475 2207 25 0.4932174807483847 2208 1 0.05754979125443782 2208 2 0.2181655705887735 2208 3 0.3265383392874348 2208 4 0.05761621772669656 2208 5 0.13695006690740286 2208 6 -0.11294437380719557 2208 7 -0.009710248464280369 2208 8 0.15459833768653652 2208 9 0.4540169316720366 2208 10 0.3219348427187896 2208 11 0.27546926204521327 2208 12 0.11963958446813905 2208 13 0.14417825271612372 2208 14 0.5116497472287023 2208 15 0.19254087591838953 2208 16 0.3591427511191796 2208 17 0.20064742009655134 2208 18 0.17886364071463134 2208 19 0.02437244559132523 2208 20 0.1675109746428518 2208 21 0.37278608423993376 2208 22 0.25412570587947414 2208 23 0.889628340813978 2208 24 0.599834486853041 2208 25 0.5243097776526682 2209 1 -0.42168387499182103 2209 2 -0.3562113516102663 2209 3 0.3432596785864739 2209 4 0.3495300329790467 2209 5 0.4425629612599806 2209 6 0.7070309869297589 2209 7 0.7722954811885714 2209 8 0.7837721615421387 2209 9 0.767893635863345 2209 10 0.6200225198683569 2209 11 0.5300800524037115 2209 12 0.7683878689375021 2209 13 0.8584008429991243 2209 14 0.8194280875021889 2209 15 0.637427643580672 2209 16 0.9652662810301322 2209 17 0.9655794865464742 2209 18 0.9890798634161087 2209 19 0.8201802593395655 2209 20 0.7772028082780835 2209 21 0.8368194906326849 2209 22 0.8742865926588479 2209 23 0.8425948554117184 2209 24 0.896039981818376 2209 25 0.8034006415192749 2210 1 -0.07761036136749766 2210 2 -0.42584753280756615 2210 3 0.4808960971507297 2210 4 0.6254488858492948 2210 5 1.1110928236410942 2210 6 0.9092460521786947 2210 7 1.135856686796906 2210 8 0.9824631367426875 2210 9 1.499039147200314 2210 10 1.2105678974166807 2210 11 1.054928056633159 2210 12 0.8227968252242235 2210 13 0.8832650313390076 2210 14 1.1921427562404219 2210 15 0.9942252397008033 2210 16 1.2699800380634798 2210 17 1.0583393898733888 2210 18 1.1494563058249554 2210 19 1.239634639029589 2210 20 1.1349269447209238 2210 21 1.0917772231309115 2210 22 1.1237983719938924 2210 23 1.1682110654167346 2210 24 0.9892921518378515 2210 25 1.2705475716632577 2211 1 -1.2376562205071728 2211 2 -0.401758792833963 2211 3 0.17510363091050252 2211 4 0.4603136775371155 2211 5 0.667667941250329 2211 6 0.6522487258849949 2211 7 0.4892512784644154 2211 8 0.466547815390264 2211 9 0.9040802681262932 2211 10 0.8544530634233851 2211 11 0.5938998642616795 2211 12 0.41826760059904444 2211 13 0.529878900767379 2211 14 0.6018120966133864 2211 15 0.45044752961179313 2211 16 0.7265957689329383 2211 17 0.7232588402406037 2211 18 0.8612206135380873 2211 19 0.657223565147298 2211 20 0.7281597602351866 2211 21 0.8855041666446368 2211 22 0.6623894303077691 2211 23 1.1284777591589048 2211 24 0.9217599150625446 2211 25 1.0085032016764544 2212 1 1.1054175180889523 2212 2 0.19419876581016027 2212 3 0.5978669641215966 2212 4 0.5052772299633546 2212 5 0.940203706323409 2212 6 0.7221795793979382 2212 7 0.8715576010692487 2212 8 0.8257958015039222 2212 9 1.081937908670504 2212 10 0.7406975168320145 2212 11 0.475484977190078 2212 12 0.6861929207631761 2212 13 0.5951606003774401 2212 14 0.6316119099952928 2212 15 0.6689231286844048 2212 16 0.3588562519396916 2212 17 0.8116885182225175 2212 18 0.8632823767938831 2212 19 0.7006652429173315 2212 20 0.6915675370757981 2212 21 1.086742039524303 2212 22 1.0814652225178847 2212 23 1.3382540211739737 2212 24 1.2295330885073597 2212 25 0.7086816705116448 2213 1 0.46642299684643973 2213 2 0.23984363215083704 2213 3 -0.2534716017575569 2213 4 -0.9296085519340238 2213 5 -1.8913641359491642 2213 6 -0.5911514306324506 2213 7 -0.1597324676873697 2213 8 -0.013651544633880519 2213 9 -0.7101900395680616 2213 10 -0.03189130749633051 2213 11 -0.2797433676903651 2213 12 -0.26217422831762605 2213 13 -0.2646653272450976 2213 14 -7.325203879113094 2213 15 -3.0859479535729406 2213 16 -1.6078766800028745 2213 17 -0.990846073785761 2213 18 -5.22643803050012 2213 19 -2.6349913933637445 2213 20 -4.909204267085837 2213 21 -1.9325435667372162 2213 22 -1.3640025467678996 2213 23 -4.017619734342954 2213 24 -1.8080788483017187 2213 25 -1.3261992894362251 2214 1 0.13678921612731898 2214 2 -0.02338711844238972 2214 3 -1.5829012166465866 2214 4 -2.0885800305605713 2214 5 -0.46909836964533724 2214 6 -0.8158736253219345 2214 7 -0.7292856664406848 2214 8 -0.8560982137234241 2214 9 -5.246987753588492 2214 10 -1.6829312401300776 2214 11 -1.2627357610306618 2214 12 -1.155707254470336 2214 13 -1.5425155372986978 2214 14 -2.791286512903131 2214 15 -2.133350006922065 2214 16 -1.7570685306517575 2214 17 -2.006390633189966 2214 18 -1.1487296352181318 2214 19 -4.573765364846608 2214 20 -2.5176836721754086 2214 21 -4.103723484274553 2214 22 -6.1754583677390364 2214 23 -6.872847523260901 2214 24 -5.871467679160073 2214 25 -3.452050156886852 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24348.0 3 14.0 4 464.0 5 2946.0 6 3556.0 7 2910.0 8 2268.0 9 1893.0 10 1582.0 11 1553.0 12 1419.0 13 1480.0 14 1335.0 15 1446.0 16 1551.0 17 1636.0 18 1782.0 19 2160.0 20 2796.0 21 3556.0 22 4702.0 23 5736.0 24 6629.0 25 7950.0 26 8862.0 27 9973.0 28 11695.0 29 14172.0 30 17106.0 31 21351.0 32 27562.0 33 35363.0 34 46186.0 35 61008.0 36 81826.0 37 121099.0 38 194932.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.525256819660505 19.04302671630783 9.594997127205701 29.83671933682596 2 13.077906774691023 20.200808010599708 41.264607180868396 25.456678033840873 3 19.531515143287674 23.179343768241857 28.34978211585192 28.939358972618546 4 12.11851763361131 16.728411569940526 36.64877897134665 34.504291825101504 5 13.253497782622329 38.3430548636384 34.81357602544385 13.589871328295422 6 33.37097677231804 37.08950374686172 16.62827399156224 12.911245489257997 7 29.28903583994908 30.7493733347297 20.13938286901846 19.822207956302762 8 24.87303500072085 39.95800022305581 18.49771911833111 16.671245657892232 9 25.9081177689941 15.992203503254887 19.10712974121398 38.99254898653703 10 15.614323932172654 29.820713319117804 32.72562174840144 21.839341000308107 11 35.13550560303352 22.1637738809201 23.955496021486553 18.745224494559825 12 24.589965921536745 23.98209082432966 31.111424767483363 20.316518486650224 13 30.30520582970413 21.88134035966761 24.905170272797474 22.908283537830783 14 20.408412698628258 23.33468683244541 26.15218309447922 30.104717374447116 15 22.630211796894297 32.7241537488566 22.300559960262635 22.345074493986466 16 21.607822646861237 28.30891583597413 28.84431385588361 21.238947661281017 17 21.121375845347686 29.59622815124853 27.805577157188864 21.47681884621492 18 20.999252203015345 27.904499132094063 30.83339327471293 20.262855390177666 19 24.141061831605235 26.463954182726233 26.450518430052654 22.944465555615874 20 23.738467676923953 28.057982579711798 27.69548967078424 20.50806007258001 21 22.28416792654096 26.795558366334348 26.535802596471992 24.3844711106527 22 22.91059066536201 29.065735491899947 26.52843806109002 21.495235781648024 23 22.01121806833712 28.56698880500294 27.459160449862726 21.96263267679722 24 23.84965630492166 26.480195970658688 28.561773507318367 21.10837421710129 25 22.651649528328132 27.802243885094192 28.09375623433359 21.45235035224409 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 220.0 1 220.0 2 225.5 3 231.0 4 231.0 5 231.0 6 330.5 7 430.0 8 430.0 9 430.0 10 728.5 11 1027.0 12 1027.0 13 1027.0 14 1667.5 15 2308.0 16 2308.0 17 2308.0 18 4132.0 19 5956.0 20 5956.0 21 5956.0 22 10208.0 23 14460.0 24 14460.0 25 14460.0 26 22584.5 27 30709.0 28 30709.0 29 30709.0 30 43217.5 31 55726.0 32 55726.0 33 55726.0 34 63238.0 35 70750.0 36 70750.0 37 70750.0 38 76044.5 39 81339.0 40 81339.0 41 81339.0 42 86630.0 43 91921.0 44 91921.0 45 91921.0 46 95776.0 47 99631.0 48 99631.0 49 99631.0 50 95857.0 51 92083.0 52 92083.0 53 92083.0 54 81438.0 55 70793.0 56 70793.0 57 70793.0 58 61358.0 59 51923.0 60 51923.0 61 51923.0 62 42892.0 63 33861.0 64 33861.0 65 33861.0 66 26421.0 67 18981.0 68 18981.0 69 18981.0 70 14007.0 71 9033.0 72 9033.0 73 9033.0 74 6289.5 75 3546.0 76 3546.0 77 3546.0 78 2448.5 79 1351.0 80 1351.0 81 1351.0 82 884.0 83 417.0 84 417.0 85 417.0 86 267.5 87 118.0 88 118.0 89 118.0 90 73.5 91 29.0 92 29.0 93 29.0 94 16.0 95 3.0 96 3.0 97 3.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08549943203948716 2 0.030264084674294665 3 0.029449804369156687 4 0.029042664216587705 5 0.02252842177548392 6 0.3753832206686056 7 0.21795569500859743 8 0.2178199816244078 9 0.22324851699199424 10 0.012757058113828244 11 0.0018999873786552705 12 0.002442840915413919 13 0.0 14 0.0656852779477965 15 0.0012214204577069595 16 0.0016285606102759461 17 0.002714267683793243 18 0.002171414147034595 19 9.499936893276352E-4 20 6.785669209483108E-4 21 1.357133841896622E-4 22 0.0 23 0.0 24 2.714267683793244E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 736847.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.57542890181462 #Duplication Level Percentage of deduplicated Percentage of total 1 91.1588834438683 71.6284836481248 2 6.370335341201111 10.011036633665297 3 1.194398499661884 2.8155112317184923 4 0.45662695801844966 1.435186362977223 5 0.22064746863090182 0.8668734741886399 6 0.12145332351259268 0.572594819193169 7 0.08662015131640248 0.4764350878858485 8 0.06073492764717324 0.38178183913578523 9 0.044537847323820476 0.31496224102494574 >10 0.23846682788643678 3.420056163120635 >50 0.02338813176210694 1.2741534540139394 >100 0.01888312776182805 2.84811911402885 >500 0.003291554371370754 1.6186485844697003 >1k 0.0017323970375635548 2.336157346452695 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 2799 0.3798617623468644 No Hit GTCCTACAGTGGACATTTCTAAATT 2737 0.3714475325271054 No Hit GTCCTACAGTGTGCATTTCTCATTT 1891 0.2566340095026512 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1678 0.2277270586702531 No Hit GTATCAACGCAGAGTACTTTTTTTT 1667 0.22623421144416683 No Hit CTTTAGGACGTGAAATATGGCGAGG 1611 0.21863426192954574 No Hit CTGTAGGACCTGGAATATGGCGAGA 1585 0.21510571394061456 No Hit GATATACACTGTTCTACAAATCCCG 1106 0.15009900291376638 No Hit GGTATCAACGCAGAGTACTTTTTTT 1086 0.1473847352299731 No Hit CTGAAGGACCTGGAATATGGCGAGA 1002 0.1359848109580415 No Hit TATCAACGCAGAGTACTTTTTTTTT 827 0.1122349687248506 No Hit GTGTATATCAATGAGTTACAATGAG 741 0.10056361768453967 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 2.714267683793243E-4 13 0.0 0.0 0.0 0.0 2.714267683793243E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACCG 40 0.0051366193 14.307445 5 TAGGACC 535 0.0 13.906301 4 GATATAC 395 0.0 13.279358 1 TAGGACT 130 1.2732926E-11 13.206872 4 TGTAGGA 1435 0.0 12.833771 2 ACTGTTC 415 0.0 12.591219 8 CTGTAGG 1440 0.0 12.517303 1 GTAGGAC 1430 0.0 12.474861 3 TCCAACG 240 0.0 12.236692 18 GACGTGG 725 0.0 12.188696 7 ATCCCGT 140 5.4569682E-11 12.188573 10 GTTACAA 400 0.0 12.0788 15 CACTGTT 420 0.0 11.990542 7 GTCCTAA 530 0.0 11.876255 1 CCAACGA 185 0.0 11.77795 19 AGGACCT 940 0.0 11.770663 5 TTACAAT 420 0.0 11.729179 16 TAGGACG 1295 0.0 11.711112 4 GGACCTG 890 0.0 11.680935 6 AGGACGT 1240 0.0 11.615184 5 >>END_MODULE