FastQCFastQC Report
Thu 26 May 2016
SRR1926519_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926519_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465831
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC23030.4943853028244149No Hit
CCCATGTACTCTGCGTTGATACCAC21340.45810605133621424No Hit
GAGTACATGGGAAGCAGTGGTATCA10740.2305557165581509No Hit
GTCCTACAGTGGACATTTCTAAATT8100.1738828029907842No Hit
CATGTACTCTGCGTTGATACCACTG7870.16894539006635453No Hit
GCGTTGATACCACTGCTTCCCATGT7360.1579972135817496No Hit
ACGCAGAGTACATGGGAAGCAGTGG6810.1461903565885482No Hit
GTATCAACGCAGAGTACATGGGAAG6060.13009009705236446No Hit
CTGTAGGACGTGGAATATGGCAAGA5880.12622603476368038No Hit
GCTTCCCATGTACTCTGCGTTGATA5650.12128862183925072No Hit
GNACATGGGAAGCAGTGGTATCAAC5250.11270181675328607No Hit
GTATCAACGCAGAGTACTTTTTTTT5230.11227247649898783No Hit
ACTCTGCGTTGATACCACTGCTTCC4910.10540303243021612No Hit
GTCCTAAAGTGTGTATTTCTCATTT4660.1000362792514882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGAC250.004048480720.3508857
CAGACTA503.0720366E-718.3760729
TAGGACC1550.017.2953054
TACACCA453.382027E-416.1823315
GTACATA708.505034E-515.2343981
GTCCTAA2650.015.0906781
CTGTAGG3700.014.4109171
TCCTAAA2850.014.3813222
GGACCTG2750.014.3622796
TTAAACA450.005152530514.2974534
TTGTAGA1406.2755134E-1014.28224851
GAGTGTC508.902916E-414.245627
TGTAGGA4200.013.9875492
AGGACCT2950.013.7530225
TGTAGAA2250.013.3586512
GTATACA802.645354E-413.3300981
GTACTGA600.00818870313.3300971
GTCCACA600.00818870313.3300971
TCTACAC755.5660246E-513.11833
GTAAATG500.00910824913.11833
Produced by FastQC (version 0.11.5)