##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926519_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 465831 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.293795389315008 27.0 7.0 31.0 2.0 31.0 2 16.418458625553043 16.0 2.0 30.0 2.0 31.0 3 20.073488024626958 24.0 15.0 31.0 2.0 31.0 4 22.187149416848598 28.0 2.0 35.0 2.0 37.0 5 22.845074715937756 30.0 2.0 35.0 2.0 37.0 6 23.561111218446175 31.0 2.0 35.0 2.0 37.0 7 23.279642187832067 30.0 7.0 35.0 2.0 37.0 8 23.54346962739706 31.0 8.0 35.0 2.0 37.0 9 26.125775227496668 31.0 17.0 37.0 2.0 39.0 10 26.799611876410115 32.0 23.0 37.0 2.0 39.0 11 23.984024249137562 30.0 7.0 37.0 2.0 39.0 12 25.079127408867166 31.0 10.0 37.0 2.0 39.0 13 21.78685188405237 27.0 2.0 37.0 2.0 39.0 14 25.11001629346265 31.0 17.0 38.0 2.0 40.0 15 26.186771597424816 31.0 17.0 38.0 2.0 40.0 16 26.531207669734304 31.0 17.0 38.0 2.0 40.0 17 25.88877726042277 31.0 11.0 38.0 2.0 40.0 18 25.945993289411824 31.0 12.0 38.0 2.0 40.0 19 26.331517224057652 31.0 16.0 39.0 2.0 40.0 20 26.592440176802317 31.0 16.0 39.0 2.0 40.0 21 24.49663504575694 30.0 2.0 38.0 2.0 40.0 22 25.418012111688572 31.0 2.0 38.0 2.0 40.0 23 25.4075812902104 31.0 2.0 38.0 2.0 40.0 24 24.468287855466897 31.0 2.0 39.0 2.0 40.0 25 24.263198456092447 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.7341247068270995 1101 2 10.561214120043934 1101 3 7.177573970425076 1101 4 8.333378676916638 1101 5 8.440603293630446 1101 6 7.829745703383761 1101 7 7.424130447844799 1101 8 6.241410158946991 1101 9 5.869329041612371 1101 10 5.284540400692986 1101 11 8.099900903711802 1101 12 5.957796307581493 1101 13 10.450707400973187 1101 14 7.974387883571538 1101 15 6.8155660619760745 1101 16 6.47905816401153 1101 17 2.9781085953059083 1101 18 5.489391592210641 1101 19 5.990679194023375 1101 20 4.045354937957349 1101 21 0.8996557102071847 1101 22 4.890452865800782 1101 23 5.9441996575504845 1101 24 7.259195749129464 1101 25 8.010576701133644 1102 1 5.288698226047309 1102 2 9.899952153351167 1102 3 6.464230076701011 1102 4 7.415151202500084 1102 5 7.464791688601057 1102 6 6.506339737363017 1102 7 6.28575332789136 1102 8 6.270779745621699 1102 9 5.342212538301748 1102 10 4.732494474993736 1102 11 7.395201829846069 1102 12 4.83512926890106 1102 13 9.395935874426705 1102 14 6.6987001831266255 1102 15 4.486614390764242 1102 16 4.982256282341908 1102 17 5.992408624406988 1102 18 5.9492621074197025 1102 19 4.80613186577223 1102 20 4.726248131005491 1102 21 4.024683836247849 1102 22 4.793022760886899 1102 23 5.474601282410603 1102 24 6.736975426977544 1102 25 6.589154108497716 1103 1 4.931831090674958 1103 2 9.518689931925048 1103 3 6.456048624907538 1103 4 7.820592069456335 1103 5 7.156892355834948 1103 6 6.60293490402222 1103 7 6.709687171725793 1103 8 6.21972217244241 1103 9 5.070649837721092 1103 10 4.353907159901112 1103 11 5.254608036464674 1103 12 4.193230861604775 1103 13 7.899554162904604 1103 14 6.191514357741884 1103 15 5.1972065421830465 1103 16 4.7919990912920944 1103 17 2.644926051600873 1103 18 1.6795816930480534 1103 19 3.4440208989548005 1103 20 3.8909519060267606 1103 21 5.89263869720347 1103 22 1.7219810102562363 1103 23 3.921328552423887 1103 24 5.450823142410499 1103 25 1.9540638751897603 1104 1 5.692591170086239 1104 2 10.453480133886446 1104 3 7.207539079134101 1104 4 7.823910326587676 1104 5 7.591312369866266 1104 6 7.360354335903164 1104 7 7.136118291395011 1104 8 7.18197195973362 1104 9 6.12810061616063 1104 10 5.227628294945074 1104 11 8.127561927169673 1104 12 5.231722942866998 1104 13 9.556519816032544 1104 14 7.18166855295631 1104 15 6.338195899280056 1104 16 6.029701537816361 1104 17 6.066617870149081 1104 18 4.170673299633815 1104 19 5.0421415981323285 1104 20 4.2651775713947835 1104 21 3.7850244688512547 1104 22 5.331952201276195 1104 23 5.666815394332744 1104 24 6.690746716515257 1104 25 5.6426820890329985 1105 1 3.720683910973964 1105 2 9.805891373345025 1105 3 6.566056665886752 1105 4 6.131020028238872 1105 5 2.6114414653709552 1105 6 4.926511352887548 1105 7 5.986167307528998 1105 8 3.131948928056204 1105 9 3.95276163711355 1105 10 3.6971438029411985 1105 11 6.799877260361271 1105 12 4.132683777204274 1105 13 5.193283688189016 1105 14 3.741819617137917 1105 15 4.391561476961769 1105 16 4.783247825582404 1105 17 5.036340072686109 1105 18 2.6459644436585705 1105 19 2.1005564281703606 1105 20 1.9158342694790207 1105 21 2.359502417496522 1105 22 4.180817152968729 1105 23 1.3448915996571955 1105 24 4.687985868004638 1105 25 5.509437929630167 1106 1 6.824492723520638 1106 2 11.341622329282293 1106 3 8.25813097507271 1106 4 9.326665099187341 1106 5 9.104451640875062 1106 6 8.074978025777767 1106 7 7.948861015520034 1106 8 7.639790600946423 1106 9 6.716566248764035 1106 10 6.136767589348967 1106 11 8.365469586724227 1106 12 6.173980920594865 1106 13 5.398427452192003 1106 14 6.46631084193254 1106 15 6.667361981069316 1106 16 6.717935558965525 1106 17 7.119809546173787 1106 18 6.865624637833328 1106 19 6.420349650724507 1106 20 5.913862182100381 1106 21 6.304073926085021 1106 22 6.868398837061335 1106 23 7.177240834160557 1106 24 8.025632899371331 1106 25 7.415559568913213 1107 1 4.131632951713499 1107 2 -13.853089209179247 1107 3 -5.5006792936059785 1107 4 2.253825110228931 1107 5 4.693112157989528 1107 6 5.105083610982035 1107 7 4.924494756778774 1107 8 5.144253243566958 1107 9 3.481742684170733 1107 10 2.9790223740379638 1107 11 5.8044935561758315 1107 12 3.07591204867731 1107 13 4.673570072729362 1107 14 4.1540983833385 1107 15 3.743474191227577 1107 16 3.4823988916854383 1107 17 4.046710769950007 1107 18 -0.7641600511618876 1107 19 1.9954152101650635 1107 20 2.316598426119519 1107 21 -0.8590080577206471 1107 22 2.122924738791184 1107 23 -0.9083717440507044 1107 24 2.7925115799013263 1107 25 3.3872367404259123 1108 1 5.074129021029279 1108 2 10.432962800277016 1108 3 0.43569735077738514 1108 4 5.797551851324091 1108 5 7.25802217642698 1108 6 7.202151716448796 1108 7 7.36576920337237 1108 8 7.10439780225575 1108 9 5.240687111648196 1108 10 4.397332010152748 1108 11 6.743228611092295 1108 12 2.3838514652393457 1108 13 3.588814340717189 1108 14 4.957996830294061 1108 15 4.972568041262075 1108 16 4.847746092698934 1108 17 0.2966570411728746 1108 18 3.198662462892429 1108 19 4.414016112808518 1108 20 5.014224179736313 1108 21 4.731168625121207 1108 22 2.0182130078196536 1108 23 4.769436574295128 1108 24 5.7010312672564005 1108 25 5.938759809866113 1109 1 3.9628301925032687 1109 2 -13.853089209179247 1109 3 -5.392148181254832 1109 4 2.356829491246895 1109 5 2.110306169411043 1109 6 2.937144589053201 1109 7 4.456809216512589 1109 8 4.735013926008456 1109 9 3.871071521716889 1109 10 3.1407223039891647 1109 11 1.4130424775430868 1109 12 1.8692740347387868 1109 13 3.1612996606287282 1109 14 4.226934291064051 1109 15 4.15732010762018 1109 16 4.077620732662798 1109 17 4.506557374957904 1109 18 4.699061362399085 1109 19 3.020962243776612 1109 20 1.7885907801152676 1109 21 1.3396663578468058 1109 22 3.1796969139844116 1109 23 3.6756511939620466 1109 24 5.06692477282046 1109 25 5.144351442362346 1110 1 3.5020945334753897 1110 2 9.467311291446537 1110 3 6.6708524057071585 1110 4 7.772381499495417 1110 5 8.405150531315034 1110 6 4.115793769014505 1110 7 6.721769759623239 1110 8 7.7420698923683915 1110 9 5.887815466536249 1110 10 5.417704698235074 1110 11 3.461278951795954 1110 12 6.244858279312492 1110 13 10.445162553761275 1110 14 8.203581589020246 1110 15 7.152098009277648 1110 16 6.759232673146716 1110 17 7.199174076355742 1110 18 7.2165320220636175 1110 19 6.22044081034711 1110 20 6.590430548808769 1110 21 4.4567978839169236 1110 22 6.433523812992785 1110 23 6.6775161181694145 1110 24 7.719109420503514 1110 25 7.99327239504203 1111 1 5.611585691901951 1111 2 9.572677661986399 1111 3 7.629054759876862 1111 4 8.131021517309097 1111 5 8.428058000006729 1111 6 7.623793799727743 1111 7 7.373490852707388 1111 8 7.468801927043639 1111 9 5.79457621808919 1111 10 5.3796740310667595 1111 11 7.140497607016819 1111 12 6.361329021113978 1111 13 8.241885451248162 1111 14 6.311759756514888 1111 15 6.019292431753598 1111 16 5.499974551414795 1111 17 6.9136377080425895 1111 18 6.5495203433548 1111 19 5.498008629016152 1111 20 5.309356814002221 1111 21 7.555215384320036 1111 22 6.974741746452555 1111 23 6.97654327635027 1111 24 6.434872222165101 1111 25 7.09884915126338 1112 1 -2.384677416683985 1112 2 7.9338566036286355 1112 3 5.924173983788133 1112 4 -0.28851820888390023 1112 5 4.1837608015419825 1112 6 3.715567500957757 1112 7 6.231928702143755 1112 8 6.439477982530356 1112 9 4.9475545492566475 1112 10 3.6384609391026608 1112 11 -0.287387792965049 1112 12 4.354926591303467 1112 13 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5.308333140617158 2204 8 5.38940210055792 2204 9 3.6977846831074714 2204 10 3.4457953671267454 2204 11 4.9851155657452075 2204 12 5.072788710027655 2204 13 3.461315952580506 2204 14 4.342694874566572 2204 15 4.4144603687926995 2204 16 4.290014521669491 2204 17 -0.7840973108580727 2204 18 2.7343115065201964 2204 19 3.5332939980438525 2204 20 4.021343242706443 2204 21 5.414383755144847 2204 22 4.790322399609895 2204 23 3.654161482956205 2204 24 5.142923594787025 2204 25 5.4468459908246345 2205 1 6.157709506737422 2205 2 10.351759275669236 2205 3 7.364663609105346 2205 4 8.1446225583948 2205 5 8.0251057784042 2205 6 7.38371200013674 2205 7 7.584191726475744 2205 8 6.980361696517516 2205 9 5.7108654911882795 2205 10 5.0922600135913925 2205 11 7.463903444533088 2205 12 6.286980629219574 2205 13 9.56747756874212 2205 14 7.2384524503241465 2205 15 6.0472381465704785 2205 16 5.519256945911913 2205 17 6.329135012374252 2205 18 6.135473122681812 2205 19 5.146627331377186 2205 20 5.19573718210038 2205 21 -0.8009697802086855 2205 22 3.7079486622361593 2205 23 4.887545321340042 2205 24 6.727216524079957 2205 25 6.894573263551909 2206 1 3.7924727874664725 2206 2 8.565003700356206 2206 3 5.618496312669098 2206 4 1.4435719545569086 2206 5 5.827042505084204 2206 6 5.883549000951735 2206 7 6.016459637863463 2206 8 5.803534253382004 2206 9 4.614602617957189 2206 10 3.4968684450946874 2206 11 3.5310146544315835 2206 12 4.536388645815855 2206 13 8.070716807091383 2206 14 0.38811237475179894 2206 15 2.613322461603449 2206 16 3.370967696449071 2206 17 3.9124587139375606 2206 18 -1.63773722035738 2206 19 0.6127190554640194 2206 20 2.160920556183509 2206 21 3.3978121713361027 2206 22 -2.4381326891752657 2206 23 -3.201729332286689 2206 24 1.020269795177235 2206 25 2.5419319358494548 2207 1 1.899504818344056 2207 2 -13.853089209179247 2207 3 -6.077554801414953 2207 4 -3.8632447943782147 2207 5 1.6756946863515978 2207 6 2.354781182526679 2207 7 3.633042498345386 2207 8 3.7102187576953334 2207 9 1.1733469091413937 2207 10 0.9422314258269537 2207 11 -1.154621426821972 2207 12 -2.153545385646062 2207 13 1.0290213080217079 2207 14 -5.270684199189862 2207 15 -1.3519156556021912 2207 16 -0.238158720182998 2207 17 0.930530095278769 2207 18 1.0239579711666629 2207 19 0.4977308190844454 2207 20 0.34139755945887273 2207 21 -3.1684254691738083 2207 22 -3.935687701400205 2207 23 -0.7896873830624713 2207 24 0.8985601490084854 2207 25 1.5630695471425327 2208 1 4.751047790390757 2208 2 6.316105656631722 2208 3 6.308266734878831 2208 4 8.087063066156194 2208 5 3.5787820981948713 2208 6 6.125213361474742 2208 7 6.516172915259734 2208 8 6.45988080828867 2208 9 4.942700291937896 2208 10 4.410141276630181 2208 11 6.440615724862635 2208 12 6.001682372439404 2208 13 8.344308656751739 2208 14 3.2993795745418204 2208 15 4.734382165523133 2208 16 5.137796601511081 2208 17 5.813868932674097 2208 18 0.6329816973432472 2208 19 3.116052740662571 2208 20 3.97225993589268 2208 21 6.5323663818789335 2208 22 5.50888639428241 2208 23 1.3189261070265452 2208 24 2.998434656394938 2208 25 4.1340697452887625 2209 1 3.5750618215003414 2209 2 5.109919422140111 2209 3 5.494466695455902 2209 4 0.4492849654493156 2209 5 5.334561370079275 2209 6 5.781656924098929 2209 7 6.171356095193751 2209 8 4.963974893842181 2209 9 4.722596612512493 2209 10 3.6408905856498315 2209 11 -0.24535187064084596 2209 12 3.3277099943879023 2209 13 2.7786975366082025 2209 14 4.37142857407709 2209 15 4.693494545329958 2209 16 4.142436709017694 2209 17 1.4996868937029504 2209 18 3.7323015798801826 2209 19 4.171936900558517 2209 20 4.3079936555899 2209 21 6.524809840910024 2209 22 5.252968278871066 2209 23 1.4152230854173133 2209 24 -2.809854805741068 2209 25 0.8897382301102184 2210 1 -1.7715593725314562 2210 2 -13.853089209179247 2210 3 -7.624220469778734 2210 4 -1.8360460322737922 2210 5 -0.284812451514032 2210 6 -1.1699917035669642 2210 7 -0.762734680450663 2210 8 -20.92751709136127 2210 9 -12.878754518431732 2210 10 -7.011876590545214 2210 11 -10.346774766145124 2210 12 -4.658392146153201 2210 13 -5.065018823754272 2210 14 -3.9474686655969684 2210 15 -5.7611649318325995 2210 16 -5.015841659186691 2210 17 -4.52291404967481 2210 18 -4.8215446743359855 2210 19 -10.2189907542409 2210 20 -7.030664934301736 2210 21 -5.438790848029754 2210 22 -10.278280293992799 2210 23 -8.083512879718157 2210 24 -10.739050912187482 2210 25 -8.228779309800665 2211 1 0.2706465696744864 2211 2 4.930244124154088 2211 3 0.6924258468675824 2211 4 1.6954150991873398 2211 5 0.9434740767724996 2211 6 -0.10987774345300139 2211 7 -20.643687061403043 2211 8 -13.242901706745887 2211 9 -8.815404905082119 2211 10 -6.788392667061288 2211 11 -9.874674644045001 2211 12 -4.718567156328213 2211 13 -6.6326622913977396 2211 14 -4.882246850375154 2211 15 -5.308519429187097 2211 16 -11.592561235906269 2211 17 -7.637321864082626 2211 18 -5.879888182679494 2211 19 -8.601204836454983 2211 20 -6.5129807666175665 2211 21 -4.421086330325238 2211 22 -5.908764624477129 2211 23 -7.932454678659955 2211 24 -21.94672286985944 2211 25 -21.75391158493294 2212 1 -1.8727344678925988 2212 2 -13.853089209179247 2212 3 -8.380752798472486 2212 4 -19.646892593120352 2212 5 -20.11550028220186 2212 6 -20.882954666529926 2212 7 -10.346833436232044 2212 8 -9.723686722004226 2212 9 -5.664910324423378 2212 10 -6.229383231444466 2212 11 -6.456496174088148 2212 12 -4.034793611131956 2212 13 -6.827271011451057 2212 14 -4.545668927613921 2212 15 -4.569850938535023 2212 16 -4.682278518168008 2212 17 -5.057629486237033 2212 18 -4.02510723631168 2212 19 -4.513383446718407 2212 20 -4.569187578501534 2212 21 -2.6182959834172443 2212 22 -3.8960160187736648 2212 23 -3.972627957273726 2212 24 -3.090361720748632 2212 25 -3.400970408462353 2213 1 -18.73319958417167 2213 2 -13.853089209179247 2213 3 -8.14988184544011 2213 4 -4.822254911960933 2213 5 -20.11550028220186 2213 6 -10.261215536095143 2213 7 -20.643687061403043 2213 8 -10.208676511651126 2213 9 -7.384022519351905 2213 10 -6.427634584564078 2213 11 -5.764028044267967 2213 12 -4.241978527565671 2213 13 -19.096764855500304 2213 14 -11.78246981581551 2213 15 -6.06649044367985 2213 16 -5.333865583732354 2213 17 -3.9020375831461713 2213 18 -3.964447825934787 2213 19 -4.925731034894227 2213 20 -4.919335281667735 2213 21 -3.022814312488002 2213 22 -3.7835137883567285 2213 23 -3.4556430844570585 2213 24 -2.595998232178278 2213 25 -3.4162304255126514 2214 1 -18.73319958417167 2214 2 -13.853089209179247 2214 3 -12.293384670298867 2214 4 -19.646892593120352 2214 5 -20.11550028220186 2214 6 -20.882954666529926 2214 7 -16.30300909530135 2214 8 -14.898703532039237 2214 9 -13.679355361405456 2214 10 -13.462490885227304 2214 11 -14.371741136874206 2214 12 -12.123260767801485 2214 13 -19.096764855500304 2214 14 -16.568296394051814 2214 15 -23.675186095853764 2214 16 -17.971939766979716 2214 17 -23.407834684595446 2214 18 -17.546663497759894 2214 19 -14.633321307554505 2214 20 -13.435449258577584 2214 21 -10.947009841841968 2214 22 -11.908569057332409 2214 23 -12.043754113688205 2214 24 -10.775536429181473 2214 25 -10.591199720526161 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 84629.0 3 6.0 4 433.0 5 1591.0 6 2413.0 7 2341.0 8 1913.0 9 1688.0 10 1553.0 11 1382.0 12 1431.0 13 1465.0 14 2208.0 15 4729.0 16 6919.0 17 7230.0 18 7448.0 19 8597.0 20 10339.0 21 11931.0 22 12371.0 23 13535.0 24 14287.0 25 14834.0 26 12396.0 27 9769.0 28 10211.0 29 11743.0 30 11753.0 31 13439.0 32 17104.0 33 21883.0 34 26220.0 35 30329.0 36 30087.0 37 31101.0 38 24523.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.418379882882505 18.477027618273098 12.71162350358793 24.39296899525647 2 12.470467074574424 20.13219980748908 42.83906490849 24.5582682094465 3 20.455539657513647 25.355655399309196 28.376561830725343 25.81224311245181 4 11.35903797959788 17.098160623760332 37.45299523917367 34.089806157468125 5 11.601654130506251 39.626461144494186 35.3757016830506 13.396183041948968 6 31.693305687800066 36.37635501695973 18.338651072438676 13.591688222801526 7 27.40953665258999 31.618344630161943 21.368158321208462 19.603960396039604 8 24.171002319312983 40.08812925004237 20.032920778861982 15.707947651782666 9 26.484196862797347 16.69177488991423 19.767729235212833 37.05629901207559 10 15.168238109560736 31.108684564987122 33.28429385328259 20.43878347216955 11 32.18035345996513 23.738790578958763 23.802600243844076 20.278255717232028 12 24.28835709088089 26.44284616104036 31.235871501664054 18.032925246414695 13 28.93518222894259 22.162482471846403 24.45512664968979 24.447208649521215 14 19.93954868840295 25.17655274758849 27.062982846667538 27.820915717341016 15 24.148608179638202 31.76687699601074 22.909396306896664 21.17511851745439 16 19.588944011339475 29.19361268603827 30.95854004252303 20.25890326009922 17 21.149010821965632 29.719088462609207 29.449975474924766 19.6819252405004 18 20.884213832225758 27.82955187357282 32.418556669552835 18.86767762464859 19 22.734089254497817 27.816939783511852 27.8036435923257 21.64532736966463 20 22.76078995356127 28.97910149564139 28.626236051696186 19.63387249910115 21 20.614163246407042 27.609024989997703 27.733997455596565 24.042814307998686 22 21.35224402564601 30.19780258162822 27.067583822145767 21.382369570580003 23 20.212034334690156 29.666109322547506 28.67471018822184 21.44714615454049 24 21.838730536321975 29.336995973927287 28.642935021168743 20.181338468582002 25 21.673150078445698 27.95245975628103 28.057985342474584 22.316404822798685 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 881.0 1 881.0 2 1508.5 3 2136.0 4 2136.0 5 2136.0 6 2908.5 7 3681.0 8 3681.0 9 3681.0 10 4184.0 11 4687.0 12 4687.0 13 4687.0 14 5541.0 15 6395.0 16 6395.0 17 6395.0 18 8544.5 19 10694.0 20 10694.0 21 10694.0 22 14928.5 23 19163.0 24 19163.0 25 19163.0 26 24892.0 27 30621.0 28 30621.0 29 30621.0 30 37224.0 31 43827.0 32 43827.0 33 43827.0 34 47445.0 35 51063.0 36 51063.0 37 51063.0 38 52764.5 39 54466.0 40 54466.0 41 54466.0 42 55566.5 43 56667.0 44 56667.0 45 56667.0 46 60060.0 47 63453.0 48 63453.0 49 63453.0 50 56181.5 51 48910.0 52 48910.0 53 48910.0 54 39477.0 55 30044.0 56 30044.0 57 30044.0 58 24272.0 59 18500.0 60 18500.0 61 18500.0 62 14745.5 63 10991.0 64 10991.0 65 10991.0 66 8300.5 67 5610.0 68 5610.0 69 5610.0 70 4039.0 71 2468.0 72 2468.0 73 2468.0 74 1739.0 75 1010.0 76 1010.0 77 1010.0 78 693.0 79 376.0 80 376.0 81 376.0 82 252.0 83 128.0 84 128.0 85 128.0 86 86.0 87 44.0 88 44.0 89 44.0 90 28.5 91 13.0 92 13.0 93 13.0 94 8.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.15498539169785 2 36.21592380069167 3 5.779563833235659 4 13.741034838814937 5 13.152409350172059 6 14.687515429415388 7 9.262157305975773 8 7.5349214629339825 9 0.10991110510034756 10 1.506340282205349 11 5.801674856332017 12 5.8914069694803475 13 15.953854509468027 14 1.6338543377319241 15 2.8692809194750883 16 3.0721441896310036 17 2.840729792564256 18 1.5718146709858296 19 1.5140684067827175 20 0.2893753313970088 21 4.493689771612452 22 1.6632641451513532 23 0.11356049726188253 24 9.036109662087753 25 0.11613653878767191 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 465831.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.97679110240226 #Duplication Level Percentage of deduplicated Percentage of total 1 96.7899551160905 83.21689751827004 2 2.009653643772809 3.4556714303767255 3 0.4604285355503726 1.1875850405579813 4 0.20232804660675618 0.6958206478906473 5 0.11929681341313113 0.5128378603001517 6 0.07141610693749811 0.36840766245072576 7 0.05303066264659189 0.3191584343071599 8 0.04180384521910729 0.28753283741442753 9 0.029106432719067005 0.2252229917061015 >10 0.18473603911659733 3.074815900116091 >50 0.017288726643648406 1.0553647473672807 >100 0.017812626591287985 3.048531092256283 >500 0.002357553519435153 1.3109854822712574 >1k 7.858511731450509E-4 1.241168354715152 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2303 0.4943853028244149 No Hit CCCATGTACTCTGCGTTGATACCAC 2134 0.45810605133621424 No Hit GAGTACATGGGAAGCAGTGGTATCA 1074 0.2305557165581509 No Hit GTCCTACAGTGGACATTTCTAAATT 810 0.1738828029907842 No Hit CATGTACTCTGCGTTGATACCACTG 787 0.16894539006635453 No Hit GCGTTGATACCACTGCTTCCCATGT 736 0.1579972135817496 No Hit ACGCAGAGTACATGGGAAGCAGTGG 681 0.1461903565885482 No Hit GTATCAACGCAGAGTACATGGGAAG 606 0.13009009705236446 No Hit CTGTAGGACGTGGAATATGGCAAGA 588 0.12622603476368038 No Hit GCTTCCCATGTACTCTGCGTTGATA 565 0.12128862183925072 No Hit GNACATGGGAAGCAGTGGTATCAAC 525 0.11270181675328607 No Hit GTATCAACGCAGAGTACTTTTTTTT 523 0.11227247649898783 No Hit ACTCTGCGTTGATACCACTGCTTCC 491 0.10540303243021612 No Hit GTCCTAAAGTGTGTATTTCTCATTT 466 0.1000362792514882 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 2.146701271491163E-4 13 0.0 0.0 0.0 0.0 2.146701271491163E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAC 25 0.0040484807 20.350885 7 CAGACTA 50 3.0720366E-7 18.376072 9 TAGGACC 155 0.0 17.295305 4 TACACCA 45 3.382027E-4 16.182331 5 GTACATA 70 8.505034E-5 15.234398 1 GTCCTAA 265 0.0 15.090678 1 CTGTAGG 370 0.0 14.410917 1 TCCTAAA 285 0.0 14.381322 2 GGACCTG 275 0.0 14.362279 6 TTAAACA 45 0.0051525305 14.297453 4 TTGTAGA 140 6.2755134E-10 14.2822485 1 GAGTGTC 50 8.902916E-4 14.24562 7 TGTAGGA 420 0.0 13.987549 2 AGGACCT 295 0.0 13.753022 5 TGTAGAA 225 0.0 13.358651 2 GTATACA 80 2.645354E-4 13.330098 1 GTACTGA 60 0.008188703 13.330097 1 GTCCACA 60 0.008188703 13.330097 1 TCTACAC 75 5.5660246E-5 13.1183 3 GTAAATG 50 0.009108249 13.1183 3 >>END_MODULE