FastQCFastQC Report
Thu 26 May 2016
SRR1926519_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926519_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465831
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC57701.2386466336504012No Hit
CCCATGTACTCTGCGTTGATACCAC57521.234782571361717No Hit
GAGTACATGGGAAGCAGTGGTATCA27880.5985003144917362No Hit
CATGTACTCTGCGTTGATACCACTG20400.43792705938419724No Hit
GTATCAACGCAGAGTACTTTTTTTT18660.400574457260251No Hit
GCGTTGATACCACTGCTTCCCATGT17010.3651538862806468No Hit
ACGCAGAGTACATGGGAAGCAGTGG16700.35849911233902426No Hit
GTATCAACGCAGAGTACATGGGAAG16470.3535616994145946No Hit
GTCCTACAGTGGACATTTCTAAATT16160.34690692547297197No Hit
CTGTAGGACGTGGAATATGGCAAGA15810.3393934710227529No Hit
GCTTCCCATGTACTCTGCGTTGATA12630.2711283705893339No Hit
GGTATCAACGCAGAGTACTTTTTTT12440.2670496381735007No Hit
ACTCTGCGTTGATACCACTGCTTCC12420.26662029791920244No Hit
TATCAACGCAGAGTACATGGGAAGC10500.22540363350657214No Hit
CTTTAGGACGTGAAATATGGCGAGG10430.22390094261652832No Hit
GGTATCAACGCAGAGTACATGGGAA10330.22175424134503716No Hit
GTCCTAAAGTGTGTATTTCTCATTT10120.2172461686749057No Hit
GTCCTACAGTGTGCATTTCTCATTT9460.20307794028306403No Hit
TATCAACGCAGAGTACTTTTTTTTT9210.19771118710433613No Hit
GATATACACTGTTCTACAAATCCCG8810.18912438201837148No Hit
CTGTAGGACCTGGAATATGGCGAGA8550.18354295871249446No Hit
CAGTGGTATCAACGCAGAGTACATG8450.1813962574410033No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8350.17924955616951213No Hit
ACATGGGAAGCAGTGGTATCAACGC7170.1539184811659164No Hit
CATGGGAAGCAGTGGTATCAACGCA6880.14769304747859202No Hit
GTGGTATCAACGCAGAGTACATGGG6800.14597568646139908No Hit
GCAGTGGTATCAACGCAGAGTACAT6550.14060893328267118No Hit
CTGAAGGACCTGGAATATGGCGAGA6360.13653020086683798No Hit
GTGTATATCAATGAGTTACAATGAG6130.1315927879424083No Hit
ATACCACTGCTTCCCATGTACTCTG5710.12257664260214542No Hit
GTGTATATCAATGAGTTACAATGAA5700.12236197247499629No Hit
GTTCTACAGTGTGGTTTTTATCATT5630.12085928158495247No Hit
GGGAAGCAGTGGTATCAACGCAGAG5450.1169952192962684No Hit
AAGCAGTGGTATCAACGCAGAGTAC4910.10540303243021612No Hit
CTGCTTCCCATGTACTCTGCGTTGA4750.10196831039583024No Hit
GATATACACTGTTCTACAATGCCGG4670.10025094937863731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT651.9708023E-716.02887519
TCCAACG1400.015.56216918
TAGGACC3000.014.3103314
GTCCTAG801.2220698E-714.3056911
TGTACTG500.001449865213.3563095
CCAACGA802.058876E-613.02346119
CTGTAGG8350.012.9065321
GTAGGAC8500.012.7951193
GTGTAGG751.4153575E-512.7161691
TGTAGGA8950.012.6833912
AGGACAG1251.3078534E-912.2114825
CTCTAGG1251.3151293E-912.2075221
CTAGGAC1103.571222E-812.1420983
GTCTAGA550.0029746612.1381621
AGGTGTG550.003057552512.0937197
AGGACGT8150.011.9399075
GTATTAA802.7444026E-511.9214091
GGACGTG7950.011.878996
TAGGACG8200.011.8671044
GACGTGG4500.011.8249697
Produced by FastQC (version 0.11.5)