FastQCFastQC Report
Thu 26 May 2016
SRR1926518_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926518_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737089
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18730.2541077129084819No Hit
CTGTAGGACGTGGAATATGGCAAGA14000.18993635775326997No Hit
GTCCTACAGTGTGCATTTCTCATTT10970.14882870318238367No Hit
GTCCTAAAGTGTGTATTTCTCATTT10450.14177392418011936No Hit
CTTTAGGACGTGAAATATGGCGAGG9190.12467965198232507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGGAC250.00270468621.819593
GTCTAGA601.0308813E-619.9716911
TAGGACC3850.017.2416784
AGGACGG504.051523E-516.6206725
GTACTAT654.5700483E-516.3870281
GTCTTAA901.6580998E-716.273231
GGTATCA1650.016.138741
TGTAGGA11000.015.9884862
CTGTAGG10850.015.8300961
GTCCTAA4750.015.6970481
GTGTAGC604.6207957E-415.5335381
CGCAGAC400.0033604215.24966057
GTCCTAC11100.015.2336621
ACTGTCG400.004480232514.6043768
ATTTAGA2100.014.5825041
CTTATAC753.5306475E-614.5463923
GGACGGG507.649937E-414.536356
AGGACCT7300.014.3723295
TTTAGAA2100.014.2828112
TCCTACA12100.014.1969632
Produced by FastQC (version 0.11.5)