##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926518_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 737089 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.874700341478437 26.0 2.0 30.0 2.0 31.0 2 15.625710056723136 16.0 2.0 30.0 2.0 31.0 3 19.031062734622278 19.0 10.0 30.0 2.0 31.0 4 21.058215493651378 27.0 2.0 35.0 2.0 35.0 5 21.45141631471912 28.0 2.0 35.0 2.0 35.0 6 21.974903980387715 29.0 2.0 35.0 2.0 36.0 7 21.789606139828432 29.0 2.0 35.0 2.0 36.0 8 22.159520763435623 29.0 2.0 35.0 2.0 37.0 9 24.697820751632435 31.0 11.0 37.0 2.0 39.0 10 25.301776311951475 31.0 10.0 37.0 2.0 39.0 11 22.579867560091117 29.0 2.0 35.0 2.0 39.0 12 23.569079174970728 30.0 2.0 37.0 2.0 39.0 13 20.44125607626759 25.0 2.0 35.0 2.0 39.0 14 23.565273664374317 29.0 2.0 37.0 2.0 40.0 15 24.602594801984562 30.0 2.0 37.0 2.0 40.0 16 24.978316051385924 31.0 2.0 38.0 2.0 40.0 17 24.28097692408922 30.0 2.0 37.0 2.0 40.0 18 24.339414914616825 30.0 2.0 37.0 2.0 40.0 19 24.69459590361544 30.0 2.0 38.0 2.0 40.0 20 24.90550259195294 31.0 2.0 38.0 2.0 40.0 21 22.85226614425124 30.0 2.0 36.0 2.0 40.0 22 23.72801113569732 30.0 2.0 37.0 2.0 40.0 23 23.693993534023708 30.0 2.0 37.0 2.0 40.0 24 22.831918533582783 30.0 2.0 38.0 2.0 40.0 25 22.612215078504768 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.9166670745232 1101 2 9.396337116731454 1101 3 6.308326727320473 1101 4 7.715227079404013 1101 5 7.38898201100649 1101 6 6.889448333811671 1101 7 6.695104543766888 1101 8 5.102497676761619 1101 9 5.042584665699195 1101 10 4.877136224294116 1101 11 7.357121518271079 1101 12 5.17470572511629 1101 13 9.340750573561603 1101 14 6.853715726672458 1101 15 5.877777683959739 1101 16 5.9306729054119955 1101 17 1.8926859416398436 1101 18 4.262472532855078 1101 19 4.927595794391969 1101 20 2.6543965676219337 1101 21 0.17109224284809343 1101 22 3.892331801154036 1101 23 5.089192420758145 1101 24 6.1736277412716625 1101 25 6.733927599821861 1102 1 4.560727127694268 1102 2 9.165396562372058 1102 3 5.930255660837197 1102 4 7.070981121911704 1102 5 6.810919206937495 1102 6 5.764401553966131 1102 7 5.323603311695113 1102 8 5.7126708958468235 1102 9 4.933430882728516 1102 10 4.362683074613191 1102 11 6.944437011269898 1102 12 4.671348511785613 1102 13 8.995566346024436 1102 14 6.647969460554673 1102 15 4.376043792022397 1102 16 4.652562470954813 1102 17 5.3920771961173415 1102 18 5.52775921432929 1102 19 4.262279465431234 1102 20 4.589679992732869 1102 21 3.392167717572395 1102 22 4.6361325603241 1102 23 4.855530129930884 1102 24 6.174327616363399 1102 25 5.939065120417144 1103 1 3.986072632146726 1103 2 8.069871240928316 1103 3 5.406224411374268 1103 4 6.9730804189952025 1103 5 6.22307060282704 1103 6 5.44184439136237 1103 7 5.559133739902123 1103 8 5.236057048170771 1103 9 4.5429083479080035 1103 10 3.836020491720358 1103 11 4.444726108084797 1103 12 3.3976499384876 1103 13 7.511590043370145 1103 14 5.787441794419145 1103 15 4.904877976892138 1103 16 4.678718674076322 1103 17 2.4273684903135546 1103 18 1.2357849347389092 1103 19 3.2896828972743606 1103 20 3.612277420200872 1103 21 5.88599295333054 1103 22 1.7489324905971415 1103 23 3.8047000353821083 1103 24 4.93797900120466 1103 25 0.8138690133420674 1104 1 4.9756045051313365 1104 2 9.334061619831258 1104 3 6.387455830662649 1104 4 6.6267671132200725 1104 5 6.455127729694581 1104 6 6.228626105192834 1104 7 6.368982723698338 1104 8 6.38482309952288 1104 9 5.2996193985208535 1104 10 4.12991129426047 1104 11 6.948607514115427 1104 12 4.0046106869825095 1104 13 8.22546755149892 1104 14 6.072437682682551 1104 15 5.222677336365443 1104 16 4.8407204849511025 1104 17 5.300968941932528 1104 18 3.3947276869020016 1104 19 3.7474841384933875 1104 20 3.1016345596541157 1104 21 2.393875512846858 1104 22 4.437776671054113 1104 23 4.6971619330860115 1104 24 5.52124380559475 1104 25 4.750145038557459 1105 1 3.092103005719501 1105 2 8.947433485800174 1105 3 6.1189983905266985 1105 4 5.393574845309381 1105 5 2.2315319342015734 1105 6 4.650171481631595 1105 7 5.361880931741432 1105 8 2.9242089915604907 1105 9 3.6207231171095344 1105 10 3.3973249449436658 1105 11 6.474151953273864 1105 12 3.5435634419002753 1105 13 4.74468534846412 1105 14 3.3409542945561874 1105 15 4.021883961706571 1105 16 4.067858300661907 1105 17 4.914275718989991 1105 18 2.384550148020253 1105 19 2.281987178737271 1105 20 1.5107207350060108 1105 21 2.1379648479695454 1105 22 3.828060435039351 1105 23 1.335609598050901 1105 24 4.009577505792855 1105 25 4.867357150302102 1106 1 6.373415913855212 1106 2 10.816893186015507 1106 3 7.656005032924412 1106 4 8.885925917421833 1106 5 8.326903298242843 1106 6 7.469423913787253 1106 7 7.0227223488798 1106 8 6.9853723580707445 1106 9 6.211573863064125 1106 10 5.51171517002766 1106 11 7.813931288192393 1106 12 5.111814604495226 1106 13 4.411986795482758 1106 14 5.550698348312615 1106 15 5.795940734216721 1106 16 5.71890446076684 1106 17 5.852035680833026 1106 18 5.819381703267187 1106 19 5.798999993584818 1106 20 5.457434256178214 1106 21 5.844573577777567 1106 22 6.1224021222224 1106 23 6.592927107467393 1106 24 7.245172200291659 1106 25 6.344848750860429 1107 1 3.387087820625517 1107 2 -13.013396007287385 1107 3 -5.188570704582412 1107 4 1.31848543500697 1107 5 4.044925304354361 1107 6 4.306149952803366 1107 7 4.188265472260422 1107 8 5.024396606621291 1107 9 3.270024837305126 1107 10 2.533359935152504 1107 11 5.060650704461114 1107 12 2.14441943561517 1107 13 3.661394814816532 1107 14 3.292152099459976 1107 15 3.1575636558940197 1107 16 2.9515604148491263 1107 17 3.628126400853475 1107 18 -1.8386719061301449 1107 19 1.0870023638312531 1107 20 1.3847571557796883 1107 21 -1.6638245306728265 1107 22 1.1541494818375853 1107 23 -1.96636739565227 1107 24 1.5872219987045249 1107 25 2.4843668007517934 1108 1 4.93093771439667 1108 2 10.016611494588085 1108 3 0.4235388113461873 1108 4 5.687237546699013 1108 5 7.0351614129998445 1108 6 7.056987471343309 1108 7 7.3062241604057405 1108 8 6.796266291599789 1108 9 5.4040260857088285 1108 10 4.665927809945632 1108 11 6.461184425677679 1108 12 2.2300564937346614 1108 13 3.1678447097330107 1108 14 4.697025455203491 1108 15 4.626624432538563 1108 16 4.737882949681307 1108 17 -0.14775764649423806 1108 18 3.6828390059490204 1108 19 4.525673100217642 1108 20 4.810009842997292 1108 21 4.651968348844765 1108 22 1.7311433486248937 1108 23 4.521816864153138 1108 24 5.360022259838509 1108 25 5.808913968077974 1109 1 3.606061410024072 1109 2 -13.013396007287385 1109 3 -5.154254905970225 1109 4 2.06732241356967 1109 5 1.489887198426704 1109 6 2.3277143732149987 1109 7 3.5327759242605943 1109 8 3.9407148732821717 1109 9 3.3826490923947006 1109 10 2.5028677516735485 1109 11 0.46173838220092733 1109 12 1.1538786352976729 1109 13 2.364085483980187 1109 14 3.195553417819653 1109 15 3.2608595550884942 1109 16 2.982243593050921 1109 17 3.591748107279706 1109 18 3.7058218028261436 1109 19 2.1498321862929757 1109 20 1.1777467812936067 1109 21 0.3179435347614721 1109 22 2.7105195899232335 1109 23 3.0031441415451923 1109 24 3.9301995320200795 1109 25 4.4250269727710645 1110 1 3.772581539396505 1110 2 9.36190665615087 1110 3 6.458554223794941 1110 4 7.802788831614674 1110 5 8.657343592927393 1110 6 4.359119905178158 1110 7 6.730674704034385 1110 8 7.631686405708219 1110 9 6.120736134891825 1110 10 5.683950475418296 1110 11 3.3740972785882413 1110 12 6.1306654232067395 1110 13 10.429407679167863 1110 14 7.979437462563684 1110 15 7.533521859074373 1110 16 7.118914042805677 1110 17 7.634302275265863 1110 18 7.643682122297115 1110 19 6.503042139814102 1110 20 6.679483264621464 1110 21 4.662750705450865 1110 22 6.662187853092412 1110 23 7.263087532909918 1110 24 7.788431420239991 1110 25 8.27234035736295 1111 1 5.262189863818467 1111 2 9.44732100262871 1111 3 7.438960286852364 1111 4 7.941791459776827 1111 5 8.544642902365322 1111 6 7.6542700871917795 1111 7 7.213909319681829 1111 8 7.499934771648864 1111 9 6.323075069922844 1111 10 5.57656336357088 1111 11 7.1788449620932475 1111 12 6.626333480601517 1111 13 8.121783423244338 1111 14 6.0455330626545525 1111 15 5.804937317395932 1111 16 5.8009673706721365 1111 17 7.081638375280082 1111 18 6.768437636727995 1111 19 5.7122054312409 1111 20 5.446636963164064 1111 21 7.930575758134157 1111 22 7.184333305152926 1111 23 7.372860860850253 1111 24 7.083893731138829 1111 25 7.67919389503912 1112 1 -1.6022075133586497 1112 2 7.710024639862075 1112 3 5.659412687365268 1112 4 0.06282258317384759 1112 5 3.8557440625143045 1112 6 3.0814766444840522 1112 7 6.029229256826227 1112 8 5.934129725100689 1112 9 4.4483096912789435 1112 10 3.3579544375578507 1112 11 0.0926753107255962 1112 12 3.9422069889855926 1112 13 7.790952913594452 1112 14 5.61172889993987 1112 15 3.5154354257337026 1112 16 3.8206896683101164 1112 17 4.814836430376289 1112 18 4.962524297071493 1112 19 4.772137451282294 1112 20 4.797320229916057 1112 21 -1.5606699416985954 1112 22 2.7744484738276114 1112 23 4.15016655558216 1112 24 5.713847811758079 1112 25 5.786354591716165 1113 1 -17.354133553420283 1113 2 -13.013396007287385 1113 3 -6.963437608569265 1113 4 -5.411771619557637 1113 5 -0.7297093558145527 1113 6 -0.18615935027481356 1113 7 -19.19265055827393 1113 8 -8.71048882610732 1113 9 -4.483697091968384 1113 10 -3.482805757486126 1113 11 -4.953427331999897 1113 12 -2.6015959330877543 1113 13 -1.5513506751481252 1113 14 -1.933135825765131 1113 15 -2.231160462563018 1113 16 -2.5939587389203957 1113 17 -2.225821426778463 1113 18 -2.7966979605868794 1113 19 -2.3931395206433095 1113 20 -3.1258943325478405 1113 21 -0.6486036769547034 1113 22 -1.7386985507526944 1113 23 -2.147516304550507 1113 24 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5.812653280418047 2204 5 1.1195661282674827 2204 6 3.760558846576089 2204 7 4.699386195171705 2204 8 4.8921438214723025 2204 9 3.3265367669779202 2204 10 2.9150147754082845 2204 11 4.44670683997191 2204 12 4.592997343801695 2204 13 3.0478589322779257 2204 14 3.94243558262049 2204 15 4.261064819051732 2204 16 4.152716558542938 2204 17 -0.3796965653740365 2204 18 2.6324747686416075 2204 19 3.418882237111358 2204 20 3.7228140394522278 2204 21 5.0934227017132265 2204 22 4.629429750240611 2204 23 3.433392958789355 2204 24 4.871642699404756 2204 25 4.8850763854051245 2205 1 5.661608575515249 2205 2 9.976109240088926 2205 3 6.835893399243538 2205 4 7.524408046220447 2205 5 7.607979655041614 2205 6 7.27560415699898 2205 7 7.476764734079893 2205 8 7.190313266611906 2205 9 6.130723877098205 2205 10 5.447417410231761 2205 11 7.316920661085856 2205 12 6.7013486046184525 2205 13 9.801325231287684 2205 14 7.5105768565470825 2205 15 6.3921700168356885 2205 16 5.835173951592203 2205 17 6.5623848441509125 2205 18 6.402778361102651 2205 19 4.8256750122398735 2205 20 5.3697543518381465 2205 21 -0.2821073739839939 2205 22 4.072218310018915 2205 23 5.249133250970985 2205 24 6.976486908234492 2205 25 7.402687411438816 2206 1 3.9435650360377714 2206 2 8.349632945942014 2206 3 5.849770647701714 2206 4 1.8795139008655077 2206 5 5.60454039471632 2206 6 5.667889640092621 2206 7 6.064216553827038 2206 8 5.924864142621562 2206 9 4.4454573789441625 2206 10 3.6845616443507794 2206 11 3.2590537148262477 2206 12 4.307154832907429 2206 13 7.251647397598738 2206 14 0.17132832936301057 2206 15 2.2682943154881414 2206 16 2.9821344357153983 2206 17 4.111797776882764 2206 18 -1.1541413972809629 2206 19 0.9888028024650275 2206 20 2.218167561714278 2206 21 2.9282874286147056 2206 22 -2.464910119085019 2206 23 -3.494247842446672 2206 24 0.47293523195681786 2206 25 1.8581439815947185 2207 1 0.8676563298481987 2207 2 -13.013396007287385 2207 3 -6.053417587286544 2207 4 -4.3218686208934685 2207 5 0.41438501267408157 2207 6 0.9111013889277615 2207 7 2.1871159786910503 2207 8 2.2860593624355907 2207 9 -0.054271951835577426 2207 10 -0.25963023326166734 2207 11 -1.7376503024596097 2207 12 -2.563543851877032 2207 13 -0.037622026600878655 2207 14 -5.430074413148809 2207 15 -1.8616361540088526 2207 16 -0.8813679719985288 2207 17 0.15975678075070476 2207 18 0.08660804159314495 2207 19 -0.523138129827224 2207 20 -0.821554662915176 2207 21 -3.953684037014348 2207 22 -4.776803677042032 2207 23 -1.9540764282361067 2207 24 -0.3274986476257098 2207 25 0.10424383167227802 2208 1 4.5371872012967 2208 2 5.965471917240917 2208 3 5.853238311692973 2208 4 7.800231078100733 2208 5 3.1905453533245485 2208 6 5.625985758650984 2208 7 6.005840007763808 2208 8 6.101723410596517 2208 9 4.459634516118317 2208 10 4.080979119942942 2208 11 5.8925677432051735 2208 12 5.472600337242707 2208 13 7.805200086313004 2208 14 2.3557269230232762 2208 15 4.034245205656372 2208 16 4.446343649761985 2208 17 5.312136194890634 2208 18 0.022316139383320888 2208 19 2.599167134103471 2208 20 3.5650336932994904 2208 21 6.003255982073828 2208 22 5.238349395419611 2208 23 1.2577259357606643 2208 24 3.0823537861163075 2208 25 4.33363080766523 2209 1 3.498555456712218 2209 2 5.217313267849013 2209 3 5.3931015449758135 2209 4 1.227945015070393 2209 5 5.487245010672449 2209 6 6.040827741621321 2209 7 6.459726682567847 2209 8 4.823598144048482 2209 9 4.479051272247084 2209 10 3.663311801305902 2209 11 -0.18153334651673347 2209 12 3.472313862441606 2209 13 2.4085689593846666 2209 14 4.080596406398033 2209 15 4.531620167052861 2209 16 3.8911878386471166 2209 17 1.9944838764741881 2209 18 3.641564657743885 2209 19 4.153009396489683 2209 20 4.375123841684026 2209 21 6.624572177900234 2209 22 5.326077156121972 2209 23 1.132102911892666 2209 24 -2.8310861174116866 2209 25 0.3765767333406096 2210 1 -1.8754368115656455 2210 2 -13.013396007287385 2210 3 -6.921506096497314 2210 4 -1.5482016389119266 2210 5 -0.33407563310378663 2210 6 -0.712698057808403 2210 7 -0.6312470495019973 2210 8 -19.54506904223367 2210 9 -11.999833651155212 2210 10 -6.464372308943741 2210 11 -8.75192398927067 2210 12 -3.9509793667342166 2210 13 -4.537727673337695 2210 14 -3.374611501654769 2210 15 -4.878256173577878 2210 16 -4.523539548800457 2210 17 -4.114249896123063 2210 18 -4.251394552440576 2210 19 -9.36701435736104 2210 20 -6.672101913011886 2210 21 -4.907134459438762 2210 22 -9.536345202710937 2210 23 -7.521331140893235 2210 24 -10.322920405652424 2210 25 -8.23599262197806 2211 1 -0.285896347896319 2211 2 4.227871255100917 2211 3 0.0472073198624301 2211 4 1.0055839895241796 2211 5 0.48582546000265836 2211 6 -0.47001105326753745 2211 7 -19.19265055827393 2211 8 -12.801770910633346 2211 9 -9.119381776817322 2211 10 -7.343598535897531 2211 11 -9.198700285548869 2211 12 -4.889175791997211 2211 13 -6.54086983689715 2211 14 -4.7670785886791265 2211 15 -5.37439633443222 2211 16 -11.452362418321945 2211 17 -7.710445231469279 2211 18 -5.844949927474424 2211 19 -8.263652785274854 2211 20 -6.394145988811999 2211 21 -4.188961420559405 2211 22 -5.654224520556049 2211 23 -7.477765687861794 2211 24 -20.27613677992143 2211 25 -20.097312588561184 2212 1 -1.509050872828464 2212 2 -13.013396007287385 2212 3 -7.614675050213197 2212 4 -18.52731609171059 2212 5 -18.825303703279225 2212 6 -19.308353863990526 2212 7 -9.550352908143381 2212 8 -9.014172610553949 2212 9 -5.457012280932439 2212 10 -5.486649498872435 2212 11 -5.223405129021703 2212 12 -3.0401579267111813 2212 13 -5.769139536328506 2212 14 -3.879191053231711 2212 15 -3.998925558092253 2212 16 -4.100811710929015 2212 17 -4.129250408718736 2212 18 -3.317539025895755 2212 19 -3.9723854875363465 2212 20 -4.261050350249452 2212 21 -1.999475843796084 2212 22 -3.2974865242480256 2212 23 -3.268584391513258 2212 24 -2.3622986598169895 2212 25 -2.791829559840558 2213 1 -17.354133553420283 2213 2 -13.013396007287385 2213 3 -7.93630114864381 2213 4 -5.123194227911306 2213 5 -18.825303703279225 2213 6 -9.78416031560343 2213 7 -19.19265055827393 2213 8 -9.714423880943349 2213 9 -7.4194714553431 2213 10 -6.420135372516651 2213 11 -5.637215174222298 2213 12 -4.421106296963014 2213 13 -17.769139536328506 2213 14 -11.139204435602544 2213 15 -6.1015159358868765 2213 16 -5.4947377054824855 2213 17 -4.099037133032539 2213 18 -4.1752296778886056 2213 19 -5.011919631298571 2213 20 -4.937051922444066 2213 21 -3.070816514037624 2213 22 -3.816972847090703 2213 23 -3.5042521531011737 2213 24 -2.74656688744831 2213 25 -3.8553771046902163 2214 1 -17.354133553420283 2214 2 -13.013396007287385 2214 3 -11.844945884085096 2214 4 -18.52731609171059 2214 5 -18.825303703279225 2214 6 -19.308353863990526 2214 7 -15.57284857807591 2214 8 -14.255960131342581 2214 9 -13.508842973140043 2214 10 -13.54080679449756 2214 11 -13.600538925952309 2214 12 -11.856366597186584 2214 13 -17.769139536328506 2214 14 -15.808573469280105 2214 15 -22.135566115098346 2214 16 -17.19572248137943 2214 17 -21.81767512586408 2214 18 -16.719051318117145 2214 19 -14.175736658162549 2214 20 -13.065072789046857 2214 21 -10.733758776005756 2214 22 -11.736244290364063 2214 23 -11.547034011932219 2214 24 -10.46623578982242 2214 25 -10.264639321234451 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 159314.0 3 9.0 4 922.0 5 3317.0 6 3932.0 7 3601.0 8 3270.0 9 3112.0 10 2872.0 11 2732.0 12 2582.0 13 2806.0 14 3793.0 15 7513.0 16 10748.0 17 11375.0 18 12019.0 19 13889.0 20 16802.0 21 19477.0 22 20497.0 23 21892.0 24 22966.0 25 23285.0 26 19498.0 27 16334.0 28 17470.0 29 20059.0 30 19383.0 31 22187.0 32 27816.0 33 33896.0 34 39491.0 35 43861.0 36 41450.0 37 40373.0 38 22546.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.33391611125533 18.64180425542562 13.699711947155906 25.324567686163142 2 12.40618748486895 19.479449378405814 45.91323589998429 22.201127236740952 3 19.722011363374968 23.43178524601477 28.685477192740322 28.16072619786994 4 10.97214183254901 16.49250069205023 39.81978382867353 32.71557364672723 5 12.551545545385881 37.68513001909355 36.92654847744193 12.836775958078636 6 31.16553181274036 37.76814004251132 17.913734143708595 13.15259400103973 7 27.385279961735076 30.252458820364115 21.68410510896535 20.678156108935458 8 22.94435696923316 40.986925632387134 20.362323302516646 15.706394095863057 9 25.60605093421959 15.850019625605915 20.207311590787782 38.33661784938672 10 14.457448039177882 30.07282502713035 34.794745800992665 20.6749811326991 11 33.61859122285629 21.50217609786186 26.026044160336788 18.853188518945064 12 24.911471071902643 23.67847965167812 32.65762617083741 18.752423105581826 13 29.380770329391904 21.709752330724903 25.07403289239047 23.835444447492726 14 18.75689571472704 24.152102917163155 27.70339600158877 29.38760536652103 15 21.380938800161054 34.96650337762269 22.812793584753724 20.839764237462532 16 19.855216406009568 28.45228436337185 31.446996614721762 20.245502615896818 17 19.525123854909825 29.466777536317963 31.024170049821464 19.983928558950744 18 19.260853747271405 27.634472912486498 34.30795647476462 18.796716865477478 19 22.904629352887785 26.23295378635642 27.30796194705258 23.55445491370322 20 22.741744561002488 28.754370926704492 29.161734492564324 19.342150019728695 21 20.746927994053173 26.383643962242203 26.907253934522508 25.96217410918211 22 21.244821978125056 30.54201801806773 28.646410249689975 19.566749754117243 23 20.657278908238464 29.349647823240677 29.580131417752533 20.412941850768323 24 22.710227044164778 26.917822199642128 30.595910851009624 19.776039905183474 25 21.226337571528887 28.358513041246002 30.43378458955695 19.981364797668157 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 717.0 1 717.0 2 1659.0 3 2601.0 4 2601.0 5 2601.0 6 3706.5 7 4812.0 8 4812.0 9 4812.0 10 5624.0 11 6436.0 12 6436.0 13 6436.0 14 7839.5 15 9243.0 16 9243.0 17 9243.0 18 13207.0 19 17171.0 20 17171.0 21 17171.0 22 24535.5 23 31900.0 24 31900.0 25 31900.0 26 42254.5 27 52609.0 28 52609.0 29 52609.0 30 64774.5 31 76940.0 32 76940.0 33 76940.0 34 83464.0 35 89988.0 36 89988.0 37 89988.0 38 91302.5 39 92617.0 40 92617.0 41 92617.0 42 91125.5 43 89634.0 44 89634.0 45 89634.0 46 86610.0 47 83586.0 48 83586.0 49 83586.0 50 75292.5 51 66999.0 52 66999.0 53 66999.0 54 56980.0 55 46961.0 56 46961.0 57 46961.0 58 38934.0 59 30907.0 60 30907.0 61 30907.0 62 24553.0 63 18199.0 64 18199.0 65 18199.0 66 13814.0 67 9429.0 68 9429.0 69 9429.0 70 6741.0 71 4053.0 72 4053.0 73 4053.0 74 2786.0 75 1519.0 76 1519.0 77 1519.0 78 1031.5 79 544.0 80 544.0 81 544.0 82 352.0 83 160.0 84 160.0 85 160.0 86 104.0 87 48.0 88 48.0 89 48.0 90 30.0 91 12.0 92 12.0 93 12.0 94 8.0 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.244397894962482 2 36.11544874499552 3 5.633105364481087 4 13.74284516523785 5 13.242091524904048 6 14.66308681855244 7 9.234841382790952 8 7.460564463721478 9 0.10934907453509686 10 1.4876086876890036 11 5.6408384876181845 12 5.867405428652442 13 15.931861688344284 14 1.6281615924264234 15 2.95663074608358 16 3.015782354641027 17 2.921492519899225 18 1.5521870493251153 19 1.4988692003272333 20 0.28748224434227077 21 4.365144507651044 22 1.6483762476444501 23 0.11002741866992995 24 8.940304359446417 25 0.11504716526769496 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 737089.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.97651316419217 #Duplication Level Percentage of deduplicated Percentage of total 1 96.52763723602212 85.88692585239286 2 2.3128830171638732 4.115845324478356 3 0.48622540695990746 1.297879239693987 4 0.20683196327466333 0.7361274761233516 5 0.10768112711614734 0.4790545612192468 6 0.07008862277220734 0.3741744760050844 7 0.04589264523909788 0.2858357287279336 8 0.037443827289771255 0.2665296953412856 9 0.0292855776560068 0.23451557272475948 >10 0.15164967782698785 2.5421820568080147 >50 0.013067235297716261 0.8002526089344342 >100 0.010198231785324294 1.9116431299300365 >500 4.7804211265553315E-4 0.3012861856689441 >1k 6.37389483540711E-4 0.7677480919516858 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1873 0.2541077129084819 No Hit CTGTAGGACGTGGAATATGGCAAGA 1400 0.18993635775326997 No Hit GTCCTACAGTGTGCATTTCTCATTT 1097 0.14882870318238367 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1045 0.14177392418011936 No Hit CTTTAGGACGTGAAATATGGCGAGG 919 0.12467965198232507 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.356688269666214E-4 0.0 5 0.0 0.0 0.0 1.356688269666214E-4 0.0 6 0.0 0.0 0.0 1.356688269666214E-4 0.0 7 0.0 0.0 0.0 1.356688269666214E-4 0.0 8 0.0 0.0 0.0 1.356688269666214E-4 0.0 9 0.0 0.0 0.0 2.713376539332428E-4 0.0 10 0.0 0.0 0.0 2.713376539332428E-4 0.0 11 0.0 0.0 0.0 4.070064808998642E-4 0.0 12 0.0 0.0 0.0 4.070064808998642E-4 0.0 13 0.0 0.0 0.0 4.070064808998642E-4 1.356688269666214E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTGGAC 25 0.002704686 21.81959 3 GTCTAGA 60 1.0308813E-6 19.971691 1 TAGGACC 385 0.0 17.241678 4 AGGACGG 50 4.051523E-5 16.620672 5 GTACTAT 65 4.5700483E-5 16.387028 1 GTCTTAA 90 1.6580998E-7 16.27323 1 GGTATCA 165 0.0 16.13874 1 TGTAGGA 1100 0.0 15.988486 2 CTGTAGG 1085 0.0 15.830096 1 GTCCTAA 475 0.0 15.697048 1 GTGTAGC 60 4.6207957E-4 15.533538 1 CGCAGAC 40 0.00336042 15.2496605 7 GTCCTAC 1110 0.0 15.233662 1 ACTGTCG 40 0.0044802325 14.604376 8 ATTTAGA 210 0.0 14.582504 1 CTTATAC 75 3.5306475E-6 14.546392 3 GGACGGG 50 7.649937E-4 14.53635 6 AGGACCT 730 0.0 14.372329 5 TTTAGAA 210 0.0 14.282811 2 TCCTACA 1210 0.0 14.196963 2 >>END_MODULE