FastQCFastQC Report
Thu 26 May 2016
SRR1926518_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926518_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737089
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA42210.5726581186261089No Hit
GTCCTACAGTGGACATTTCTAAATT41860.5679097096822772No Hit
CTTTAGGACGTGAAATATGGCGAGG27190.3688835405222436No Hit
GTCCTAAAGTGTGTATTTCTCATTT25280.3429707945716189No Hit
GTCCTACAGTGTGCATTTCTCATTT24220.32858989891315704No Hit
CTGTAGGACCTGGAATATGGCGAGA20460.2775784199737074No Hit
GTATCAACGCAGAGTACTTTTTTTT17590.23864146663428706No Hit
CTGAAGGACCTGGAATATGGCGAGA15030.20391024693083198No Hit
GATATACACTGTTCTACAAATCCCG12300.1668726571689443No Hit
GTTCTACAGTGTGGTTTTTATCATT10390.14095991121831963No Hit
GGTATCAACGCAGAGTACTTTTTTT10160.13783952819808734No Hit
GTGTATATCAATGAGTTACAATGAA9830.13336245690818885No Hit
GAATATGGCAAGAAAACTGAAAATC9530.1292923920991902No Hit
CTGTAGGACATGGAATATGGCAAGA9420.12780003500255735No Hit
GTCCTTCAGTGTGCATTTCTCATTT9270.12576500259805803No Hit
GTGTATATCAATGAGTTACAATGAG9080.12318729488569223No Hit
CTGTAGAACATATTAGATGAGTGAG9030.12250895075085913No Hit
ACCTGGAATATGGCGAGAAAACTGA8970.1216949377890594No Hit
ACAGTGGACATTTCTAAATTTTCCA8810.11952423655759345No Hit
TATCAACGCAGAGTACTTTTTTTTT8730.11843888594186049No Hit
GATATACACTGTTCTACAATGCCGG8410.1140974834789286No Hit
GACCTGGAATATGGCGAGAAAACTG8130.11029875632386318No Hit
ATTCCAGGTCCTTCAGTGTGCATTT8100.10989174984296333No Hit
GCCATATTCCACGTCCTACAGTGGA7620.1033796461485655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6700.015.2296594
GTCCTAG1350.014.826191
AGAGACG400.00514365914.3044935
GTCTAAG400.005162336414.2966831
CCAACGT400.00537157914.21132919
GTCTTAG551.9043522E-413.863451
AGGACCT11600.013.6468155
TGTAGGA20400.013.41813852
CTGTAGG20450.013.0965551
GTAGGAC20600.012.9628863
GGACCTG11800.012.7689836
CTAAAAC751.4182542E-512.7159743
TATGACG604.1798662E-412.6331516
GGTATCA3500.012.5266181
CTAGGAC1404.9112714E-1112.2618323
ACTGTTC5350.012.2548378
TAAGACT550.002979379112.1371454
GATCTAC1209.502401E-911.9139021
CTATAGG1459.094947E-1111.83173751
TAGGACT2100.011.8068834
Produced by FastQC (version 0.11.5)