FastQCFastQC Report
Thu 26 May 2016
SRR1926517_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926517_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559338
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGA14650.26191676589110696No Hit
GCTTACTCTGCGTTGATACCACTGC13110.23438421848685412No Hit
GTCCTACAGTGGACATTTCTAAATT8760.15661371120860731No Hit
CTGCTTACTCTGCGTTGATACCACT7330.13104777433322964No Hit
CTGTAGGACGTGGAATATGGCAAGA6880.12300254944237653No Hit
GTCCTAAAGTGTGTATTTCTCATTT5770.10315766137827216No Hit
GTCCTACAGTGTGCATTTCTCATTT5620.1004759197479878No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGGA351.8910754E-423.393712
ATCTAGG552.3698017E-416.954341
GTGTATA2400.016.6515831
GTCCTAG901.6448212E-716.2815481
GCCTTAT500.00245148915.9855191
GATATAC1251.1459633E-1015.9855191
GCTTATT708.561926E-515.2243041
CCTCAAG658.439605E-414.3459791
CTGTAGG4650.014.3239431
ATCCCGT458.7027356E-414.29016410
TAGAACC450.005206543514.2760214
TAGGAGT601.4818739E-414.2760214
TAGGACC2100.014.2760214
GTCCTAC6850.014.1963861
TGTAGGA5200.014.171192
GGACCTG3800.013.6747836
AATAGTA600.00701606913.6463322
GTGTAAG904.818677E-513.3212661
TCCTACA7600.013.1076612
TCAGCAC500.00917723613.1042653
Produced by FastQC (version 0.11.5)