##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926517_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 559338 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.84191848220575 27.0 7.0 30.0 2.0 31.0 2 16.283588813919312 16.0 2.0 30.0 2.0 31.0 3 19.82233461699366 23.0 15.0 31.0 2.0 31.0 4 22.05607342966149 28.0 2.0 35.0 2.0 37.0 5 22.357915964944272 28.0 2.0 35.0 2.0 37.0 6 23.09515892000901 30.0 2.0 35.0 2.0 37.0 7 23.1494284314672 30.0 2.0 35.0 2.0 37.0 8 23.232038588474232 30.0 7.0 35.0 2.0 37.0 9 25.94330798193579 31.0 17.0 37.0 2.0 39.0 10 26.638567377864547 31.0 22.0 37.0 2.0 39.0 11 23.736177409723638 30.0 7.0 37.0 2.0 39.0 12 24.85698450668469 31.0 10.0 37.0 2.0 39.0 13 21.533317958014653 27.0 2.0 35.0 2.0 39.0 14 24.836896474046103 30.0 13.0 38.0 2.0 40.0 15 25.920843926212772 31.0 17.0 38.0 2.0 40.0 16 26.294639377263838 31.0 16.0 38.0 2.0 40.0 17 25.644971376877667 31.0 10.0 38.0 2.0 40.0 18 25.73173823341164 31.0 11.0 38.0 2.0 40.0 19 26.134827242204178 31.0 11.0 39.0 2.0 40.0 20 26.33861457651724 31.0 10.0 39.0 2.0 40.0 21 24.23902005585174 30.0 2.0 37.0 2.0 40.0 22 25.147476123560352 31.0 2.0 38.0 2.0 40.0 23 25.124078821749997 31.0 2.0 38.0 2.0 40.0 24 24.176342390468733 31.0 2.0 38.0 2.0 40.0 25 23.947475408429252 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.279685960572472 1101 2 10.146228248390896 1101 3 6.703626441153187 1101 4 8.175905687916444 1101 5 7.767502949039439 1101 6 7.1630705559356365 1101 7 7.084435941402731 1101 8 5.609940954766838 1101 9 5.498791334213621 1101 10 4.802791120576771 1101 11 7.433390101166221 1101 12 5.2014980911050195 1101 13 9.841608836600003 1101 14 7.171001630531254 1101 15 6.450095066536701 1101 16 6.022501478011023 1101 17 1.569989353851522 1101 18 4.095724184425148 1101 19 4.964234758869061 1101 20 3.4719464375058173 1101 21 0.6849308737402708 1101 22 4.316160134336741 1101 23 5.305697199917034 1101 24 6.810735923792581 1101 25 7.301071937872152 1102 1 5.186607777492277 1102 2 10.186155797794106 1102 3 6.618157909223797 1102 4 7.740369818174454 1102 5 7.525707585835118 1102 6 6.210581182767228 1102 7 6.362647933804773 1102 8 6.3691266933940796 1102 9 5.349321586270694 1102 10 4.839469235203055 1102 11 7.23463353878547 1102 12 4.94859278603337 1102 13 9.10923917273633 1102 14 6.6373056190344 1102 15 4.322644304986966 1102 16 5.188512836682932 1102 17 5.83006318628188 1102 18 5.818502860394744 1102 19 4.552625868789669 1102 20 5.05763340543346 1102 21 4.195194176811452 1102 22 4.850194163582717 1102 23 5.34057042731688 1102 24 6.863160563373182 1102 25 6.76899054574924 1103 1 4.393072845271213 1103 2 8.892099489139254 1103 3 6.034216303384383 1103 4 7.6037691925227335 1103 5 6.546562643186526 1103 6 6.203281912694237 1103 7 6.306339278955971 1103 8 5.565164232497313 1103 9 4.893638583142433 1103 10 4.155423354076881 1103 11 5.270087136282864 1103 12 4.127946279255479 1103 13 8.0821275981795 1103 14 6.264000092444199 1103 15 5.248608851389466 1103 16 5.144717216244974 1103 17 2.845704479295435 1103 18 1.6746029646700293 1103 19 3.5484548573193884 1103 20 3.8605531134626574 1103 21 5.86881252926192 1103 22 1.5884631938225517 1103 23 3.768099103020738 1103 24 5.156174119160461 1103 25 1.5208153632674914 1104 1 5.08396730613822 1104 2 9.885270042478561 1104 3 6.534411354506908 1104 4 7.283958695052021 1104 5 7.350581440374814 1104 6 6.755323359197611 1104 7 6.7551800800367765 1104 8 6.805659609201086 1104 9 5.654754636965915 1104 10 4.661098969710309 1104 11 7.46499050891342 1104 12 4.664291159402676 1104 13 8.838550732624466 1104 14 6.878443016871724 1104 15 6.023999245945468 1104 16 5.462868450488088 1104 17 5.697410352832154 1104 18 3.5116263250979323 1104 19 4.176263301172831 1104 20 3.5218954691062265 1104 21 2.608478274351892 1104 22 4.877831897843368 1104 23 5.22414776056943 1104 24 5.977912274455168 1104 25 5.195057666720267 1105 1 2.897684941204478 1105 2 9.045295526678357 1105 3 6.058459264802526 1105 4 5.79940110076048 1105 5 2.0444917359915316 1105 6 4.595948231776614 1105 7 5.488376818059205 1105 8 2.7544353482428825 1105 9 3.7356771649985347 1105 10 3.2772314875492476 1105 11 6.380551161308933 1105 12 3.9413247985464075 1105 13 4.849813729566364 1105 14 3.6919323135078095 1105 15 3.963607808636599 1105 16 4.2143699795400735 1105 17 4.713407294728178 1105 18 1.713417354659601 1105 19 2.158640467329814 1105 20 1.5580463355690206 1105 21 2.13372598077391 1105 22 3.618767677659882 1105 23 0.7959051509873696 1105 24 3.9015891348017533 1105 25 4.632347167229952 1106 1 5.26898005586051 1106 2 10.308451060078157 1106 3 6.894019583845399 1106 4 8.103800901358685 1106 5 7.594902503688441 1106 6 6.734792698376815 1106 7 6.125507426234677 1106 8 6.15923395243032 1106 9 4.9358217312996295 1106 10 4.318070969104582 1106 11 6.588532218138443 1106 12 4.003627889274224 1106 13 3.5917010675524033 1106 14 4.571614267645398 1106 15 4.768365535456141 1106 16 4.5586880254024855 1106 17 5.141862927828875 1106 18 4.968959727540959 1106 19 4.946022321766506 1106 20 4.434385318619867 1106 21 4.803233458341207 1106 22 5.175443649743631 1106 23 5.4408483214759045 1106 24 6.234190331212524 1106 25 5.087977276900936 1107 1 4.306318744021379 1107 2 -13.639704473321641 1107 3 -5.286745966585805 1107 4 2.06738299210857 1107 5 4.854226142430164 1107 6 5.234256078859108 1107 7 5.102980439789587 1107 8 5.621612410617129 1107 9 3.973614790753061 1107 10 3.250144968434558 1107 11 5.685168867546359 1107 12 3.075147736172159 1107 13 4.410954574636786 1107 14 3.835149617330746 1107 15 3.7620544887170837 1107 16 3.671410220988765 1107 17 4.130427415452523 1107 18 -0.9843874743142393 1107 19 1.666197811394202 1107 20 1.9484527742008098 1107 21 -1.5091633552421868 1107 22 1.4963411183037465 1107 23 -1.722964064304378 1107 24 1.3873054325884766 1107 25 2.3486509901675774 1108 1 5.761624002143538 1108 2 10.938717105099938 1108 3 1.0821245994678605 1108 4 6.456514986874367 1108 5 7.882008219508013 1108 6 7.78085032967871 1108 7 8.004874320556706 1108 8 7.421439344246878 1108 9 6.042285556606924 1108 10 5.258903815221576 1108 11 7.701764947522719 1108 12 3.2828133100349177 1108 13 4.395638904391536 1108 14 5.653078167653664 1108 15 5.409885530914323 1108 16 5.5201811683512645 1108 17 0.7935741278950097 1108 18 4.420812959055208 1108 19 5.285761346450695 1108 20 5.6216387431614265 1108 21 5.5804512554991845 1108 22 3.1709974478896505 1108 23 5.330996060078281 1108 24 6.044978745191365 1108 25 6.463514999397784 1109 1 3.9023568325315416 1109 2 -13.639704473321641 1109 3 -5.331955573233001 1109 4 2.6184679764135623 1109 5 1.03799016859341 1109 6 2.875215734388945 1109 7 4.176358009281774 1109 8 4.098371607386039 1109 9 3.734271731351722 1109 10 2.932276419628664 1109 11 1.4808594162723594 1109 12 2.0482317995913384 1109 13 3.1539380764838114 1109 14 3.898200861052217 1109 15 3.6332713405070294 1109 16 3.6375350788086216 1109 17 4.1370008161492855 1109 18 4.324254345255213 1109 19 2.979263246849147 1109 20 1.5296158235523016 1109 21 0.9891115607112155 1109 22 3.407037270136371 1109 23 3.621487178155604 1109 24 4.808674819232266 1109 25 5.004387919581049 1110 1 3.0510499047081296 1110 2 8.912954546490662 1110 3 5.99771995301942 1110 4 7.524519974587385 1110 5 8.169086105126045 1110 6 4.090664602996636 1110 7 6.182251687715095 1110 8 6.830023460860193 1110 9 5.1511985414323185 1110 10 4.948958286297945 1110 11 2.8133613802870343 1110 12 5.287487467993746 1110 13 9.661898192548637 1110 14 7.34533481611469 1110 15 6.47697172938738 1110 16 6.1332469347017025 1110 17 6.732044416730265 1110 18 6.5953537467346806 1110 19 5.552625868789669 1110 20 5.751064062367767 1110 21 3.8354444374996675 1110 22 5.856450680788139 1110 23 6.233166881957132 1110 24 7.236466089963383 1110 25 7.505174070253933 1111 1 5.584990776214539 1111 2 9.775788484424838 1111 3 7.84303270544639 1111 4 8.262554018265512 1111 5 8.798894150478453 1111 6 7.920372779060315 1111 7 7.921893920674897 1111 8 8.116582229530607 1111 9 6.584278774656482 1111 10 6.057992050929528 1111 11 7.9195753061781495 1111 12 7.158648742208378 1111 13 8.967292602805799 1111 14 6.783841770248252 1111 15 6.2248311486768415 1111 16 6.252899154590377 1111 17 7.508697033158761 1111 18 7.1333187631103065 1111 19 5.853320547812711 1111 20 5.607406496534814 1111 21 8.36407608137753 1111 22 7.768976933193084 1111 23 7.66838402422681 1111 24 7.522114285707186 1111 25 8.024707328195746 1112 1 -1.5323746070501407 1112 2 7.621075814152691 1112 3 5.489759059463715 1112 4 -0.2971553674120031 1112 5 3.6161508735685324 1112 6 2.6901350900928342 1112 7 5.289851150708074 1112 8 5.718043151117627 1112 9 4.157864023314755 1112 10 3.055461467015366 1112 11 -0.7230525347896304 1112 12 3.5577724371501027 1112 13 7.633544735726527 1112 14 5.141848124596105 1112 15 3.294383989334751 1112 16 3.5028278850842227 1112 17 4.545622900477664 1112 18 4.446807498396769 1112 19 4.258796524824682 1112 20 4.131910811955056 1112 21 -1.829797633189127 1112 22 2.01781079880978 1112 23 3.567695705402155 1112 24 5.432535746711405 1112 25 5.4763163664085965 1113 1 -18.29931505879552 1113 2 -13.639704473321641 1113 3 -7.6941983142596335 1113 4 -6.256207405237337 1113 5 -1.6004177514675746 1113 6 -1.0324279950499964 1113 7 -20.497623181940796 1113 8 -9.78394305960881 1113 9 -5.655756858992742 1113 10 -4.950993158034176 1113 11 -6.143336515899353 1113 12 -3.7433971207556667 1113 13 -2.6014327262678805 1113 14 -3.0560295185532595 1113 15 -3.4487062589751787 1113 16 -3.723155647504637 1113 17 -3.3580223672129357 1113 18 -3.9654801371615527 1113 19 -3.7045918118413965 1113 20 -4.355201210777764 1113 21 -1.636476854558694 1113 22 -2.7966150922419715 1113 23 -3.039381653810885 1113 24 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4.545296409914265 2204 7 5.748328235063251 2204 8 5.857621583060695 2204 9 4.2731468006260656 2204 10 4.030421770950056 2204 11 5.132150351592333 2204 12 5.314847065752883 2204 13 3.077089033210086 2204 14 4.824235957232506 2204 15 4.761494448312714 2204 16 4.878940829742504 2204 17 -0.43736598462404785 2204 18 3.3275509578984703 2204 19 3.9562356090302195 2204 20 4.398256861884811 2204 21 5.791555940288081 2204 22 5.366354722194295 2204 23 4.4431602117161155 2204 24 5.781755126704589 2204 25 5.863128543748168 2205 1 5.884869125388661 2205 2 10.37230753869037 2205 3 7.2970088143520755 2205 4 7.845676376035758 2205 5 7.919012256512051 2205 6 7.193458742287124 2205 7 7.459333775016162 2205 8 7.11212203492957 2205 9 6.066092580413951 2205 10 5.181481737799498 2205 11 7.643278889036662 2205 12 6.508061535283144 2205 13 9.983576492329124 2205 14 7.685819200394697 2205 15 6.546466667495459 2205 16 5.900939549109644 2205 17 6.61305994738083 2205 18 6.42302396126621 2205 19 5.128362189051536 2205 20 5.2841164056390895 2205 21 -0.6952750202270899 2205 22 3.792159069051273 2205 23 5.1506338797161 2205 24 6.825980526193145 2205 25 7.121837657720441 2206 1 4.546609394484797 2206 2 8.977591749342375 2206 3 6.473105388062962 2206 4 2.187697323424498 2206 5 6.106245215116779 2206 6 6.490105289430691 2206 7 6.444722742512486 2206 8 6.365793201125587 2206 9 5.09004098209595 2206 10 4.3944521634935825 2206 11 3.950342413794022 2206 12 5.088422214053363 2206 13 8.237712337916264 2206 14 0.6687136256350463 2206 15 2.8148443891534995 2206 16 3.4983679914685055 2206 17 4.859015644628773 2206 18 -0.7422307566724058 2206 19 1.3092249603715658 2206 20 2.684225262010372 2206 21 3.3415112690442896 2206 22 -1.785117014189023 2206 23 -2.3872399782372824 2206 24 1.5528793932510574 2206 25 3.1691205270709055 2207 1 1.634695093488741 2207 2 -13.639704473321641 2207 3 -5.99873362859849 2207 4 -4.02147707182835 2207 5 1.5781871674636108 2207 6 1.837262952588798 2207 7 2.8729351936937206 2207 8 3.1127449929129334 2207 9 0.6039766573843224 2207 10 0.26930255353909516 2207 11 -0.973788940213911 2207 12 -2.441873170996743 2207 13 0.5609152524090035 2207 14 -5.296499158573411 2207 15 -1.1814277243583255 2207 16 -0.22497519812490197 2207 17 0.8416255688398522 2207 18 0.8458589790250848 2207 19 0.2259298074313385 2207 20 0.08308186856394428 2207 21 -3.5409745268403015 2207 22 -4.151516586299508 2207 23 -1.1750491637334406 2207 24 0.5655536018068297 2207 25 1.1328700098695457 2208 1 4.726898533437488 2208 2 6.364179021823988 2208 3 6.17658547839476 2208 4 8.009236052216792 2208 5 3.8664529010400734 2208 6 6.254792891970787 2208 7 6.707231186991244 2208 8 6.984658649213756 2208 9 5.092424737814067 2208 10 4.541911099199734 2208 11 6.694191938007961 2208 12 6.164528682041553 2208 13 8.438289457721702 2208 14 3.118349789235964 2208 15 4.599258294073493 2208 16 5.187680659151724 2208 17 5.853611178223325 2208 18 0.885349393494554 2208 19 3.4114948362618556 2208 20 4.535405558869989 2208 21 7.276599767181679 2208 22 6.145427871834638 2208 23 2.217050782055331 2208 24 3.5908998144134046 2208 25 4.641055905094028 2209 1 3.147780376889127 2209 2 5.1144449042717195 2209 3 5.0679986838896625 2209 4 0.1616874078144228 2209 5 5.121520781634683 2209 6 5.579213791942589 2209 7 6.101961880299868 2209 8 4.5468046428486915 2209 9 4.132004965828408 2209 10 3.582683769706925 2209 11 -0.6788471789400283 2209 12 3.1395820599571955 2209 13 2.3857514889024607 2209 14 3.8196792014746137 2209 15 4.017483327308799 2209 16 3.3749384442703665 2209 17 1.435511029168012 2209 18 3.4896538691824226 2209 19 4.1742753221016 2209 20 3.9868679123325066 2209 21 5.904273698616233 2209 22 4.735829918323784 2209 23 -0.04904920585910233 2209 24 -2.7687303672729335 2209 25 0.3336293249062976 2210 1 -1.7710758561377133 2210 2 -13.639704473321641 2210 3 -7.364390126116632 2210 4 -1.6953796301365287 2210 5 -0.2327374998888665 2210 6 -0.7722012698845155 2210 7 -0.5086973790615055 2210 8 -20.574564651757118 2210 9 -12.586310653384633 2210 10 -6.6025448136689135 2210 11 -9.052333667041012 2210 12 -3.911953163801325 2210 13 -4.917613734442497 2210 14 -3.2630831903681603 2210 15 -5.035537263345681 2210 16 -4.324222490005884 2210 17 -4.1957388846738155 2210 18 -4.308721072804406 2210 19 -9.825917672205069 2210 20 -6.382852889237181 2210 21 -4.677662723545026 2210 22 -9.080959897090949 2210 23 -6.969014007373648 2210 24 -9.679475095541546 2210 25 -7.36976025248212 2211 1 0.15131519330531873 2211 2 4.779413173737181 2211 3 0.48275338244958377 2211 4 1.4359473192478767 2211 5 0.8270631645629578 2211 6 0.09786419816316894 2211 7 -20.497623181940796 2211 8 -12.96427053411006 2211 9 -8.740079137522475 2211 10 -6.966877756148232 2211 11 -9.538230351296109 2211 12 -4.585818058596452 2211 13 -6.80859803513952 2211 14 -4.483630711702276 2211 15 -4.936341771195334 2211 16 -11.386066995249841 2211 17 -7.690853209473502 2211 18 -5.642616258785775 2211 19 -8.521930961241612 2211 20 -6.383642740061234 2211 21 -4.132299229310124 2211 22 -5.854484423180455 2211 23 -7.458346949852967 2211 24 -21.565410865198245 2211 25 -21.36865283277005 2212 1 -0.48695807939048663 2212 2 -13.639704473321641 2212 3 -7.340641421696331 2212 4 -19.42959889923952 2212 5 -19.60150826400847 2212 6 -20.317051768223386 2212 7 -9.531948124732558 2212 8 -8.779370143747965 2212 9 -4.47289262905181 2212 10 -4.787303981558303 2212 11 -4.891084994297476 2212 12 -2.2023594119799093 2212 13 -5.143030664026314 2212 14 -3.0866512105196513 2212 15 -2.8375638160773597 2212 16 -3.1434583957459665 2212 17 -3.355382178956031 2212 18 -2.4078389382465772 2212 19 -2.8999956882765936 2212 20 -3.164672199900089 2212 21 -0.943701936846228 2212 22 -2.131268935612429 2212 23 -2.2291245972963836 2212 24 -1.0837175013538527 2212 25 -1.5585841828844664 2213 1 -18.29931505879552 2213 2 -13.639704473321641 2213 3 -8.650350954175577 2213 4 -5.839574568339275 2213 5 -19.60150826400847 2213 6 -10.555494590607815 2213 7 -20.497623181940796 2213 8 -10.70230187803449 2213 9 -8.902850815536745 2213 10 -7.759766079360727 2213 11 -6.674473169591309 2213 12 -5.339380797560651 2213 13 -18.779186270433637 2213 14 -12.243646761917981 2213 15 -7.202903140268511 2213 16 -6.2537881713115056 2213 17 -4.860760588483501 2213 18 -4.982852718333099 2213 19 -5.7709751044463395 2213 20 -5.722693323798378 2213 21 -3.91081903139154 2213 22 -4.927699475624788 2213 23 -4.843301661664697 2213 24 -3.6894984564391216 2213 25 -4.430696628390486 2214 1 -18.29931505879552 2214 2 -13.639704473321641 2214 3 -12.222939705596445 2214 4 -19.42959889923952 2214 5 -19.60150826400847 2214 6 -20.317051768223386 2214 7 -16.4010980854157 2214 8 -14.913045990238457 2214 9 -13.95292643860507 2214 10 -14.02501046869271 2214 11 -14.204139958382187 2214 12 -12.541416514194907 2214 13 -18.779186270433637 2214 14 -16.56271504813955 2214 15 -23.3123921913634 2214 16 -18.04958240141231 2214 17 -23.039592705271822 2214 18 -17.569683031440782 2214 19 -14.8082887475994 2214 20 -13.592903471380788 2214 21 -11.333913658865729 2214 22 -12.301935025042821 2214 23 -12.213873484791264 2214 24 -11.1201084198958 2214 25 -10.853852317969535 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 103431.0 3 3.0 4 591.0 5 2117.0 6 2829.0 7 2671.0 8 2363.0 9 2205.0 10 1969.0 11 1813.0 12 1753.0 13 1866.0 14 2760.0 15 5569.0 16 8566.0 17 8968.0 18 9179.0 19 10410.0 20 12522.0 21 14507.0 22 15352.0 23 16816.0 24 17544.0 25 17986.0 26 14761.0 27 11700.0 28 12865.0 29 14693.0 30 14097.0 31 16566.0 32 21114.0 33 26113.0 34 31712.0 35 35987.0 36 35478.0 37 36456.0 38 24006.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.82228409502784 18.74011499555175 13.45966918185113 23.977931727569278 2 12.139618205228684 21.110675698370933 42.924200862117225 23.825505234283156 3 20.910204908795794 24.73508071160304 29.321522525952147 25.033191853649022 4 10.532566661355967 17.27391418598348 41.694099536313814 30.499419616346753 5 11.353937856428344 39.09373098619146 36.13443471624952 13.417896441130669 6 28.32392919383825 38.59546718384203 19.144903249292337 13.935700373027379 7 25.703073904512753 32.7728178902661 22.983562766435263 18.540545438785884 8 21.94202265519154 38.34490702712941 20.4418554760354 19.27121484164365 9 24.225778159623403 18.08050976391201 22.15951601066781 35.53419606579678 10 16.2554900648195 29.877744578872793 33.34476246455609 20.522002891751615 11 30.97064539224711 22.92019440940378 26.179544584985926 19.929615613363183 12 25.79722452321964 25.51459674835892 31.291311328426364 17.396867399995074 13 28.11897092445117 22.468956900874563 25.501236624482615 23.910835550191656 14 19.065966904825487 24.312364094151757 30.264336307139494 26.35733269388326 15 22.22107607376202 33.773147337998566 23.77040272177512 20.23537386646429 16 18.474592205216307 30.2854793904415 31.374345380845025 19.865583023497162 17 19.78147228170705 29.68021815945453 30.31694620955313 20.221363349285294 18 18.660172425374014 30.327477502827467 32.342556099969684 18.669793971828838 19 21.41819085685464 28.834092026554565 27.28646671516981 22.461250401420983 20 21.413650585883218 29.015518957154768 28.1184497180409 21.452380738921114 21 21.23532433568393 27.745388315509267 26.991080184934386 24.028207163872413 22 20.539088847540413 29.25467350854751 27.91203919060356 22.294198453308525 23 19.833716673975495 30.64589169209841 29.808389342831827 19.712002291094265 24 23.942175192497086 27.337829682588673 29.09580919543393 19.624185929480312 25 20.318695147076987 29.239617436942073 29.64201391561488 20.799673500366058 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 955.0 1 955.0 2 1775.0 3 2595.0 4 2595.0 5 2595.0 6 3562.5 7 4530.0 8 4530.0 9 4530.0 10 5167.5 11 5805.0 12 5805.0 13 5805.0 14 6852.5 15 7900.0 16 7900.0 17 7900.0 18 11138.0 19 14376.0 20 14376.0 21 14376.0 22 19958.0 23 25540.0 24 25540.0 25 25540.0 26 32862.0 27 40184.0 28 40184.0 29 40184.0 30 48256.0 31 56328.0 32 56328.0 33 56328.0 34 60898.0 35 65468.0 36 65468.0 37 65468.0 38 67323.5 39 69179.0 40 69179.0 41 69179.0 42 70804.5 43 72430.0 44 72430.0 45 72430.0 46 72655.5 47 72881.0 48 72881.0 49 72881.0 50 63194.5 51 53508.0 52 53508.0 53 53508.0 54 42433.5 55 31359.0 56 31359.0 57 31359.0 58 24943.5 59 18528.0 60 18528.0 61 18528.0 62 14235.5 63 9943.0 64 9943.0 65 9943.0 66 7377.0 67 4811.0 68 4811.0 69 4811.0 70 3403.0 71 1995.0 72 1995.0 73 1995.0 74 1341.0 75 687.0 76 687.0 77 687.0 78 469.0 79 251.0 80 251.0 81 251.0 82 158.0 83 65.0 84 65.0 85 65.0 86 38.0 87 11.0 88 11.0 89 11.0 90 9.5 91 8.0 92 8.0 93 8.0 94 4.0 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.185944813332902 2 36.12806567764035 3 5.604303658968281 4 13.594105889462185 5 13.318065284318248 6 14.545230254336374 7 9.244857313466992 8 7.496182987746229 9 0.11585123842828486 10 1.4508222219838451 11 5.610203490554906 12 5.683325645673993 13 15.858389739298955 14 1.5849093034980637 15 2.9422281339726606 16 3.011953416360054 17 2.905041316699384 18 1.5180445455163067 19 1.4608340574035734 20 0.29195227215029196 21 4.293110784534575 22 1.6094025437213277 23 0.11674515230504633 24 9.051593133311165 25 0.12246620111631965 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 559338.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.5414857650715 #Duplication Level Percentage of deduplicated Percentage of total 1 96.64256740113412 85.56876505847478 2 2.2072788271935986 3.908714937150114 3 0.4527060356666195 1.2024979503821382 4 0.20744763286618487 0.7347088652967634 5 0.10932790663332047 0.4840027644449605 6 0.07124359309647092 0.3784808150402236 7 0.04821200721307105 0.29881339252531586 8 0.03620635113310523 0.25646112987656133 9 0.029474727916494234 0.2348762582022867 >10 0.1683894526433124 2.852649200693101 >50 0.01272472501331939 0.8378637567734284 >100 0.012936789836930638 2.0756922008366954 >500 0.0010603926095757547 0.6452034016682652 >1k 4.2415704383030184E-4 0.5212702686353621 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGA 1465 0.26191676589110696 No Hit GCTTACTCTGCGTTGATACCACTGC 1311 0.23438421848685412 No Hit GTCCTACAGTGGACATTTCTAAATT 876 0.15661371120860731 No Hit CTGCTTACTCTGCGTTGATACCACT 733 0.13104777433322964 No Hit CTGTAGGACGTGGAATATGGCAAGA 688 0.12300254944237653 No Hit GTCCTAAAGTGTGTATTTCTCATTT 577 0.10315766137827216 No Hit GTCCTACAGTGTGCATTTCTCATTT 562 0.1004759197479878 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTAGGA 35 1.8910754E-4 23.39371 2 ATCTAGG 55 2.3698017E-4 16.95434 1 GTGTATA 240 0.0 16.651583 1 GTCCTAG 90 1.6448212E-7 16.281548 1 GCCTTAT 50 0.002451489 15.985519 1 GATATAC 125 1.1459633E-10 15.985519 1 GCTTATT 70 8.561926E-5 15.224304 1 CCTCAAG 65 8.439605E-4 14.345979 1 CTGTAGG 465 0.0 14.323943 1 ATCCCGT 45 8.7027356E-4 14.290164 10 TAGAACC 45 0.0052065435 14.276021 4 TAGGAGT 60 1.4818739E-4 14.276021 4 TAGGACC 210 0.0 14.276021 4 GTCCTAC 685 0.0 14.196386 1 TGTAGGA 520 0.0 14.17119 2 GGACCTG 380 0.0 13.674783 6 AATAGTA 60 0.007016069 13.646332 2 GTGTAAG 90 4.818677E-5 13.321266 1 TCCTACA 760 0.0 13.107661 2 TCAGCAC 50 0.009177236 13.104265 3 >>END_MODULE