FastQCFastQC Report
Thu 26 May 2016
SRR1926517_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926517_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559338
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGA42390.7578601847183635No Hit
GCTTACTCTGCGTTGATACCACTGC29190.5218669212533388No Hit
CTGTAGGACGTGGAATATGGCAAGA18900.33789944541583083No Hit
CTGCTTACTCTGCGTTGATACCACT17840.31894847122848796No Hit
GTCCTACAGTGGACATTTCTAAATT17500.31286985686651003No Hit
GTATCAACGCAGAGTACTTTTTTTT16490.2948127965559286No Hit
CTTTAGGACGTGAAATATGGCGAGG14020.2506534510439126No Hit
GTCCTAAAGTGTGTATTTCTCATTT13080.23384787016079725No Hit
GTCCTACAGTGTGCATTTCTCATTT12220.21847255148050018No Hit
GCGTTGATACCACTGCTTACTCTGC11540.20631532275654432No Hit
CTGTAGGACCTGGAATATGGCGAGA9970.17824642702623458No Hit
GGTATCAACGCAGAGTACTTTTTTT9480.1694860710339723No Hit
GTATCAACGCAGAGTAAGCAGTGGT9120.16304989112128981No Hit
CTGAAGGACCTGGAATATGGCGAGA7980.14266865473112858No Hit
TATCAACGCAGAGTACTTTTTTTTT7850.14034447865154878No Hit
CCCATGTACTCTGCGTTGATACCAC7090.12675698772477464No Hit
GATATACACTGTTCTACAAATCCCG6670.11924811115997841No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6640.11871176283392151No Hit
ACGCAGAGTAAGCAGTGGTATCAAC6520.11656636952969404No Hit
GTAAGCAGTGGTATCAACGCAGAGT5980.10691209966067029No Hit
GTACATGGGAAGCAGTGGTATCAAC5660.10119105084939696No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCCA402.6693195E-416.6956715
TCCAACG1300.015.30116918
CCAACGA902.3719622E-914.7344619
TAGGACC3350.014.5241664
TAAGACG400.005127105414.3105754
GGCGAGG3750.013.38730919
CCAACGT500.001530946513.26101319
CTCCTAC655.223492E-513.2109521
ATCCCGT957.543531E-812.97465210
TGTAGGA9150.012.7296812
GTCCTAG905.108486E-712.7216571
AGGACCT5550.012.3767145
CGTTTCC1001.4798388E-712.32150814
CCGTTTC1001.4798388E-712.32150813
CTGTAGG9300.012.2086871
GTAGGAC9300.012.11154653
GGACCTG5650.011.8167526
CTAGACA657.7291543E-411.742014
AATACTG657.7291543E-411.742015
AGGACTG907.0933056E-611.6604695
Produced by FastQC (version 0.11.5)