##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926517_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 559338 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.37778230694142 31.0 28.0 31.0 22.0 34.0 2 28.797916465536044 31.0 28.0 33.0 23.0 34.0 3 29.124123517443834 31.0 28.0 33.0 24.0 34.0 4 32.82716532758368 35.0 33.0 37.0 27.0 37.0 5 32.49301495696699 35.0 33.0 37.0 25.0 37.0 6 32.55295009457609 35.0 33.0 37.0 25.0 37.0 7 32.41783679993135 35.0 33.0 37.0 23.0 37.0 8 32.36256610493118 35.0 33.0 37.0 23.0 37.0 9 33.58830975188526 37.0 33.0 39.0 19.0 39.0 10 33.478313649349765 37.0 33.0 39.0 19.0 39.0 11 33.67016544558031 37.0 34.0 39.0 23.0 39.0 12 33.490771233136314 37.0 33.0 39.0 21.0 39.0 13 33.61708126392271 37.0 34.0 39.0 23.0 39.0 14 34.23245157668529 38.0 33.0 40.0 18.0 41.0 15 34.363728192970974 38.0 34.0 40.0 19.0 41.0 16 34.37681687995452 38.0 34.0 40.0 19.0 41.0 17 34.32048242744101 38.0 34.0 40.0 19.0 41.0 18 34.31609331030611 38.0 34.0 40.0 18.0 41.0 19 34.35025691084818 39.0 34.0 40.0 18.0 41.0 20 34.326557466147484 39.0 34.0 40.0 18.0 41.0 21 34.23892887663631 39.0 34.0 40.0 18.0 41.0 22 34.16277456564725 39.0 34.0 40.0 18.0 41.0 23 33.81860878395532 39.0 34.0 40.0 10.0 41.0 24 33.88601346591864 39.0 34.0 41.0 9.0 41.0 25 33.65175081971903 39.0 34.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7890102363630831 1101 2 0.8430503605417528 1101 3 0.8588672531050108 1101 4 0.5158063822766223 1101 5 0.625644343458184 1101 6 0.27861076835751675 1101 7 0.321858181644636 1101 8 0.5320743523109712 1101 9 0.779254680933434 1101 10 0.7903645698487267 1101 11 0.6675700285181065 1101 12 0.8472680675909601 1101 13 0.7569006588347236 1101 14 1.0385409203973524 1101 15 0.8486506191966328 1101 16 0.9579467596853846 1101 17 0.7660806006579293 1101 18 1.0820764442117863 1101 19 0.9065985080925287 1101 20 0.8225000045066437 1101 21 0.9629953418001378 1101 22 0.8507017064995992 1101 23 1.0617343634760488 1101 24 1.1119934671226233 1101 25 1.0211763654624448 1102 1 0.47274786230924093 1102 2 0.2847545227307471 1102 3 0.380238372145719 1102 4 -0.00600373465838544 1102 5 -0.05549756128246486 1102 6 -0.23477547857189052 1102 7 0.048907786280793175 1102 8 0.12950052785273414 1102 9 0.2881797313437815 1102 10 0.3501644803484325 1102 11 0.3466508753482316 1102 12 0.1022057309529174 1102 13 -0.07875468636324712 1102 14 0.33076470515445067 1102 15 0.41855492190747157 1102 16 -0.08644198623687771 1102 17 0.07372033326250715 1102 18 0.0365375875833962 1102 19 0.03725618646332407 1102 20 0.13061700116579544 1102 21 0.14800691199887694 1102 22 0.13562941003039697 1102 23 0.44574165955727096 1102 24 0.40984556628725244 1102 25 0.35008039074362074 1103 1 0.2464704900464696 1103 2 -0.14694516444339456 1103 3 -0.15469384891789062 1103 4 -0.35532594529446726 1103 5 -0.25987712332625534 1103 6 -0.3348797538586439 1103 7 -0.11584716679532647 1103 8 -0.06028049404508096 1103 9 0.4539774372874774 1103 10 0.513876680556983 1103 11 -0.05689448440151068 1103 12 0.0646666277203849 1103 13 -0.01827502004416459 1103 14 0.321379929346314 1103 15 0.38414407727764655 1103 16 0.23472589697480117 1103 17 0.2562020850873239 1103 18 0.1533259087512775 1103 19 0.26249080585852624 1103 20 0.1410445298415013 1103 21 0.28773579625330825 1103 22 0.27014452994698246 1103 23 0.5604444749900139 1103 24 0.5214201231172808 1103 25 0.39908977551942826 1104 1 0.48727731077694614 1104 2 0.5606854268006707 1104 3 0.4205390166116345 1104 4 0.2324220805436923 1104 5 0.3269695218475803 1104 6 -0.09345854866592873 1104 7 0.31472260570171784 1104 8 0.6594953512916035 1104 9 0.7721191049905158 1104 10 0.6945439786152932 1104 11 0.5635944933499104 1104 12 0.5516513499558897 1104 13 0.6101116680090328 1104 14 0.7826795544442433 1104 15 0.44192279045045524 1104 16 0.5033086353224903 1104 17 0.5051223947455981 1104 18 0.6152059039467517 1104 19 0.5722458067673486 1104 20 0.6746916456891086 1104 21 0.8202838229418248 1104 22 0.5968790765301861 1104 23 0.9699912442099929 1104 24 0.7073043743601346 1104 25 0.9304526141882334 1105 1 0.8775382689828604 1105 2 0.8070371087578572 1105 3 0.8904625640122461 1105 4 0.6399837523072023 1105 5 0.8539664637436104 1105 6 0.4432203504166381 1105 7 0.309559506823021 1105 8 0.06145151846795471 1105 9 0.6117490744094809 1105 10 0.7919048348844058 1105 11 0.548909478059386 1105 12 0.665394469221944 1105 13 0.44005866087346135 1105 14 0.7446041212128449 1105 15 0.7621857873923545 1105 16 0.848983456933091 1105 17 0.5757849839402951 1105 18 0.6053384217856888 1105 19 0.8314751645655178 1105 20 0.8669454683190807 1105 21 0.7526273499550769 1105 22 0.5248077207707524 1105 23 1.1044163206625939 1105 24 0.8246224171735932 1105 25 1.015041808887517 1106 1 0.20705172860399657 1106 2 0.41274697914668934 1106 3 0.36535289302520724 1106 4 0.32379830863821013 1106 5 0.44639717161997794 1106 6 -0.8516610055155631 1106 7 -0.8229479707442735 1106 8 -0.5625734067563712 1106 9 -0.5896975856104589 1106 10 -0.539493968704825 1106 11 -0.7763367831569639 1106 12 -0.49917781392860405 1106 13 -0.6295465235732038 1106 14 -0.21793226961467127 1106 15 -0.07312228838033263 1106 16 -0.27777726091336774 1106 17 -0.34760484656818136 1106 18 -0.14058381151441068 1106 19 -0.30407019934276036 1106 20 -0.3644234250009575 1106 21 -0.48309249052348235 1106 22 -0.16449836098398407 1106 23 -0.23364250286682164 1106 24 -0.525733515029799 1106 25 -0.39399707446243326 1107 1 0.6813571824637457 1107 2 0.8633208109711354 1107 3 0.8646778557627499 1107 4 0.5969938126693748 1107 5 0.6875519768220784 1107 6 0.37836320755949515 1107 7 0.5890464283521979 1107 8 0.9656708343633298 1107 9 0.9398194969446863 1107 10 0.5975245531942619 1107 11 0.46183100723443715 1107 12 0.7329316925619835 1107 13 0.5976321015173198 1107 14 0.9557188093416187 1107 15 0.7597994694849106 1107 16 0.8643577024059326 1107 17 0.8738936358517648 1107 18 0.8702478785261292 1107 19 0.9118614824729576 1107 20 0.8278368164076113 1107 21 1.0663637684661467 1107 22 0.9219385054790052 1107 23 1.290679902151524 1107 24 1.0548175881997466 1107 25 1.2580317485253474 1108 1 1.3888609463055381 1108 2 1.2820710747918227 1108 3 1.026431594981279 1108 4 0.9672784576019069 1108 5 -0.09534822157107925 1108 6 0.702039531235819 1108 7 1.0781559104194258 1108 8 1.4297841858006208 1108 9 1.5428010224614255 1108 10 1.7428229168024885 1108 11 1.4069774101273183 1108 12 1.355682181509657 1108 13 1.143417302232102 1108 14 1.519021703630429 1108 15 1.407602370808938 1108 16 1.302482957432595 1108 17 1.6170237451790541 1108 18 1.502721039168307 1108 19 1.4528697699601238 1108 20 1.5582331806067913 1108 21 1.7128581191858459 1108 22 1.6981763521393844 1108 23 1.9328968401431084 1108 24 2.092885154436331 1108 25 2.0845503003960104 1109 1 0.7700544654300892 1109 2 -0.18866508559951 1109 3 0.7436339328732693 1109 4 0.7395177125997847 1109 5 -0.6618621673953697 1109 6 0.3049194099777708 1109 7 0.655750729393553 1109 8 0.7446876731179017 1109 9 1.2039444767083296 1109 10 -1.0203908808940696 1109 11 0.568953365206724 1109 12 0.8210329227224662 1109 13 0.7726960694418992 1109 14 1.1048381859986307 1109 15 0.829410447789428 1109 16 0.9973857557836681 1109 17 1.151500762414699 1109 18 1.2604690382956178 1109 19 1.201707139487148 1109 20 1.4655901914120761 1109 21 1.2330735359529896 1109 22 1.3071420990361702 1109 23 1.3914727469948787 1109 24 1.3921950399531084 1109 25 1.4268976082605604 1110 1 0.8043432741966257 1110 2 -0.017643808864665544 1110 3 0.489727423240609 1110 4 0.7917022090329553 1110 5 0.9976828349636264 1110 6 0.9675185777367687 1110 7 1.1187722284080053 1110 8 1.059636085725515 1110 9 1.525927385149835 1110 10 1.4648672957811755 1110 11 0.9796018659634527 1110 12 1.2502766381479091 1110 13 0.8910054804772116 1110 14 1.4444247677196245 1110 15 1.1818500209689944 1110 16 0.6685110898840847 1110 17 0.9830008337838834 1110 18 1.3086960860192676 1110 19 1.33548350658846 1110 20 1.443442861436914 1110 21 1.366985014188657 1110 22 1.5412602755152776 1110 23 1.629266164249664 1110 24 1.3994180376115466 1110 25 1.5304766368332992 1111 1 0.4881982287414104 1111 2 0.38444757153451903 1111 3 0.4642754412758272 1111 4 0.38673224325287947 1111 5 0.6855399043874684 1111 6 0.523232422851251 1111 7 0.7842174545091396 1111 8 0.6226124842627243 1111 9 1.178250080445693 1111 10 1.1518505089286677 1111 11 0.650965816087556 1111 12 0.9110001030247616 1111 13 0.7435073530263736 1111 14 1.037207742943231 1111 15 0.9288135539919509 1111 16 0.8029894931503989 1111 17 0.7028674789101146 1111 18 0.747511460015069 1111 19 0.5537125891127985 1111 20 0.7674746433693826 1111 21 0.91244022721866 1111 22 1.0979948435071734 1111 23 1.1709615922923717 1111 24 1.2932481717007533 1111 25 1.3103456318251432 1112 1 0.24028570224774626 1112 2 -0.02174523210456414 1112 3 0.42462067489520194 1112 4 0.5278975100074064 1112 5 0.5201512686717393 1112 6 0.5284072087328653 1112 7 0.4086457491228188 1112 8 0.6135808819176489 1112 9 0.16764435161687175 1112 10 0.6639849170199312 1112 11 0.12994027888074555 1112 12 0.11978050618293423 1112 13 0.05133997504183441 1112 14 0.6563166468801995 1112 15 0.36422685515415765 1112 16 0.3071169271589653 1112 17 0.48309504554193694 1112 18 0.6019708653175186 1112 19 0.4634915916702411 1112 20 0.16422062232318524 1112 21 0.13869921853824252 1112 22 0.19617117046274757 1112 23 0.5715272036194676 1112 24 0.0814951071119907 1112 25 0.27784673701894036 1113 1 -0.1619622762734103 1113 2 0.14155510221478096 1113 3 -1.2520837827707432 1113 4 -0.8961405661224582 1113 5 -1.1243192505421078 1113 6 -0.5878309036727813 1113 7 -0.2752769162958444 1113 8 -0.3062507716083793 1113 9 0.4169955302437174 1113 10 0.31412184688004174 1113 11 -0.11723883164617632 1113 12 0.046884109352809844 1113 13 -0.5142990272399572 1113 14 -0.015413326987811615 1113 15 0.061917521307293555 1113 16 0.06697800437039092 1113 17 -0.07551272598336567 1113 18 0.01838095177478749 1113 19 -2.4491442465577116 1113 20 -1.0210120016918225 1113 21 -0.43150896411253115 1113 22 -0.6540210687603221 1113 23 -0.3815673216492925 1113 24 -0.7719675501110288 1113 25 -0.550905846510517 1114 1 0.6210305427135445 1114 2 -0.6166339729198462 1114 3 -0.5939414094976883 1114 4 0.28405880749483714 1114 5 0.6572953820659038 1114 6 0.2574234409243701 1114 7 0.22202087547644567 1114 8 0.004173372772591222 1114 9 0.2029168448289056 1114 10 0.1951211704252458 1114 11 0.3446666524523252 1114 12 0.4005688621579253 1114 13 -0.5078751139609778 1114 14 -0.24110007304764025 1114 15 -0.30274577993656493 1114 16 -0.1858752627137079 1114 17 -3.536047245640276 1114 18 -1.0164275826293192 1114 19 -0.4328780363174829 1114 20 -0.18275210342484627 1114 21 0.26702690845397825 1114 22 -0.06233135207693152 1114 23 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2202 16 -0.10978124894926111 2202 17 0.09633120242769166 2202 18 -0.21655233451683387 2202 19 -0.08319710434204808 2202 20 -0.33052511991621714 2202 21 -0.10154071727180991 2202 22 -0.17583933805277496 2202 23 -0.016205528076902453 2202 24 -0.23471371728018653 2202 25 -0.1212439053981953 2203 1 -0.12569577357033168 2203 2 -0.07757991251873975 2203 3 -0.11198424449839095 2203 4 -0.5688673115225882 2203 5 -0.3270548128521398 2203 6 -0.4149498623493173 2203 7 -0.0190575144535714 2203 8 -0.034356992170344824 2203 9 0.43411077653713903 2203 10 0.4406920689269569 2203 11 0.0834201163572601 2203 12 0.14126680964747607 2203 13 0.23716504385217974 2203 14 0.5887317807873131 2203 15 0.652909191647673 2203 16 0.4743238824650078 2203 17 0.368550941387106 2203 18 0.15342352816866622 2203 19 0.17300707945913274 2203 20 0.35339227682971597 2203 21 0.2744784137848697 2203 22 0.5348928271537332 2203 23 0.754905682364722 2203 24 0.7088139065352976 2203 25 0.2696375535683728 2204 1 0.1639674187804303 2204 2 0.36462010470927453 2204 3 0.03999697696771065 2204 4 0.05854245676064096 2204 5 0.15474379167883257 2204 6 0.11433775932352575 2204 7 0.4377174015598655 2204 8 0.5185243929618792 2204 9 1.0228867262313415 2204 10 0.8107024057346095 2204 11 0.8585127169257873 2204 12 0.6444066149709329 2204 13 0.602261090023255 2204 14 0.8940939997553556 2204 15 0.6521210100644197 2204 16 0.7817405419533401 2204 17 0.6887809353573005 2204 18 0.7102372072107883 2204 19 0.8231836665898058 2204 20 0.855054780059973 2204 21 0.569133422829573 2204 22 0.6771316074104305 2204 23 0.8334568064925492 2204 24 0.696715534582502 2204 25 0.5009365457296227 2205 1 0.263089820534411 2205 2 0.21793901065975874 2205 3 0.37484194339162613 2205 4 0.08780957813302592 2205 5 0.07645295023009169 2205 6 0.11381294300923628 2205 7 0.16504999731351688 2205 8 0.19688595290239164 2205 9 0.5296840093444146 2205 10 0.5798237538033248 2205 11 0.2867212898712026 2205 12 0.6463974722249475 2205 13 0.5591157179305171 2205 14 0.7394069240156469 2205 15 0.727088690295254 2205 16 0.340521995471633 2205 17 0.5074366355919508 2205 18 0.7472002836475511 2205 19 0.7343620514524005 2205 20 0.9080075303811412 2205 21 0.669353075680803 2205 22 0.5522932062562376 2205 23 0.8378407450870142 2205 24 0.7680348295860071 2205 25 0.7702189860646982 2206 1 0.5574627222631783 2206 2 0.5068919795332043 2206 3 0.5312478522826254 2206 4 0.45880283779428055 2206 5 0.6893700422724365 2206 6 0.35621041005878595 2206 7 0.5204839601033413 2206 8 0.36881334749379846 2206 9 0.6404747205327368 2206 10 0.7503680555603509 2206 11 0.4679512275623665 2206 12 0.4505515268760192 2206 13 0.4593151221060623 2206 14 0.7258685347317311 2206 15 0.74665099613987 2206 16 0.5147071149847804 2206 17 0.4402780256897998 2206 18 0.5891157577697825 2206 19 0.45781313673251134 2206 20 0.49902697925347184 2206 21 0.5042635328576637 2206 22 0.6383782973115117 2206 23 0.8577503166864489 2206 24 0.7880279837739863 2206 25 0.8614314709153561 2207 1 -1.0031825431998804 2207 2 -0.03218624149594973 2207 3 0.19140165030666267 2207 4 0.3117299452006037 2207 5 0.274230423134469 2207 6 0.24000207630496817 2207 7 0.44004174032556165 2207 8 0.4111824829349615 2207 9 0.8587084652724215 2207 10 0.6741028044275552 2207 11 0.6109500871964428 2207 12 0.5134452306432635 2207 13 0.3731246507211736 2207 14 -4.574055777264313 2207 15 -1.6956111669223688 2207 16 -0.32898101007198477 2207 17 -0.22137237646579422 2207 18 0.22340948777554104 2207 19 0.44544470771272415 2207 20 0.5261485140043618 2207 21 0.8167237966555874 2207 22 0.6901579745778577 2207 23 0.9108782495965997 2207 24 0.7079066811329824 2207 25 0.5727372403596007 2208 1 0.4309557447110137 2208 2 0.8927361378840715 2208 3 0.7634237290607899 2208 4 0.7221779270644859 2208 5 0.674432483161084 2208 6 0.5348125834263513 2208 7 0.7483459145900113 2208 8 0.9527427806038773 2208 9 1.0199917928560822 2208 10 1.016244640709651 2208 11 0.8185987984477379 2208 12 0.6637828187365074 2208 13 0.5088547773783105 2208 14 0.8938856746109067 2208 15 0.7406906417612831 2208 16 0.7223718064476543 2208 17 0.7616102266587461 2208 18 0.9270859946012209 2208 19 0.737766426701441 2208 20 0.6653629440472386 2208 21 0.812559388790028 2208 22 0.6551572353338599 2208 23 1.1202124371674458 2208 24 0.8808651356201906 2208 25 0.9357602554894626 2209 1 -0.6868019799797942 2209 2 -0.38105832692679087 2209 3 -0.05640465590476751 2209 4 0.022357196290606396 2209 5 0.15116978324568464 2209 6 0.23387595207638867 2209 7 0.4753893823416959 2209 8 0.3371403151484529 2209 9 0.5636287424592723 2209 10 0.007377863930045692 2209 11 -0.011605044762191596 2209 12 0.04588409577796426 2209 13 0.007245382865157524 2209 14 0.2685543286817236 2209 15 0.34597001975749464 2209 16 0.0021909258127621456 2209 17 0.2655360212846958 2209 18 0.29820149232510573 2209 19 0.43253740522941797 2209 20 0.09905750151410331 2209 21 0.0940422877144158 2209 22 0.22863344691183585 2209 23 0.4165283538576148 2209 24 0.3359128736051247 2209 25 0.4415895267298424 2210 1 -0.1854716977945472 2210 2 -0.2852917948966258 2210 3 0.3919188209336184 2210 4 0.7215629328166173 2210 5 0.9221923773648726 2210 6 0.8933133736724557 2210 7 0.92372894203897 2210 8 1.2696497465964143 2210 9 1.3821244896918685 2210 10 1.329786340975211 2210 11 0.8625063772841912 2210 12 0.8748451890447555 2210 13 0.8586478081602777 2210 14 1.2023848521098586 2210 15 0.9293906600390827 2210 16 1.1829358378854735 2210 17 1.1271028133976628 2210 18 1.2950837152519128 2210 19 1.1342968699918714 2210 20 1.1272300751850821 2210 21 1.1595741938089006 2210 22 1.114426768389805 2210 23 1.1775359219914918 2210 24 1.2515249642389321 2210 25 1.3161868808698003 2211 1 -1.1308987058070556 2211 2 -0.30686205090600893 2211 3 0.2234037404828335 2211 4 0.26828627331560284 2211 5 0.4113950351721769 2211 6 0.2932791739460541 2211 7 0.38254270010033053 2211 8 0.30341790502257737 2211 9 0.3951692424767046 2211 10 0.5001905491701208 2211 11 0.3183968730173703 2211 12 0.24843648602866608 2211 13 0.036722526419680435 2211 14 0.5059318523052809 2211 15 0.30632864453520625 2211 16 0.3828658098742679 2211 17 0.6036841436041627 2211 18 0.492481278928544 2211 19 0.36428188725459165 2211 20 0.40056731705857374 2211 21 0.47934163566936405 2211 22 0.5951690653085677 2211 23 0.6928280853684754 2211 24 0.4774711566693881 2211 25 0.5552266828371017 2212 1 1.0650851797380092 2212 2 0.24853138793405805 2212 3 0.5824190857513756 2212 4 0.5491691069982778 2212 5 0.806213603331706 2212 6 0.6230510140321996 2212 7 0.9159782711250841 2212 8 0.7349915642345479 2212 9 1.0192342002676043 2212 10 0.9046119287059184 2212 11 0.5412710341234614 2212 12 0.8115866889015777 2212 13 0.6588092329558393 2212 14 0.6940411921510616 2212 15 0.5370004327012765 2212 16 0.39237247295140065 2212 17 0.9450298351988806 2212 18 0.7652011220145241 2212 19 0.8097177274945793 2212 20 1.0381651479529452 2212 21 1.0488905078498192 2212 22 0.9867161875899768 2212 23 1.385956366429184 2212 24 1.2778192135122666 2212 25 0.9156962711300807 2213 1 0.42321710109964883 2213 2 0.25399331313742124 2213 3 -0.1870191878125702 2213 4 -0.9728094350407304 2213 5 -1.8163595703196513 2213 6 -0.25128573230841766 2213 7 0.20740622215149074 2213 8 0.314762953991071 2213 9 -0.5743506822815192 2213 10 -0.16269613835160612 2213 11 -0.08226813751238637 2213 12 -0.4222989614349899 2213 13 -0.23273452647447357 2213 14 -7.264125805794453 2213 15 -3.0468604413181097 2213 16 -1.7454617985413563 2213 17 -0.9288865489064193 2213 18 -4.709552089163214 2213 19 -2.6160284850695206 2213 20 -4.329237013432746 2213 21 -1.9140368836215629 2213 22 -1.5378151502754775 2213 23 -4.221620175687775 2213 24 -1.9545965609285965 2213 25 -1.5784910378278099 2214 1 0.3035588610034523 2214 2 0.25297404569479554 2214 3 -1.7934692249195407 2214 4 -1.9117614214379728 2214 5 -0.4241905504134067 2214 6 -0.576591747395458 2214 7 -0.5879887557252381 2214 8 -0.7163129602965235 2214 9 -5.968957489297086 2214 10 -2.1048609308813617 2214 11 -1.108102104952195 2214 12 -1.5432207173932397 2214 13 -1.4155732567584565 2214 14 -3.024785840177117 2214 15 -2.3199583567517905 2214 16 -1.6626240076743741 2214 17 -1.7594016312463197 2214 18 -1.28197174552448 2214 19 -4.622280305189953 2214 20 -2.3574007350271238 2214 21 -3.9855875792598496 2214 22 -6.239498585535554 2214 23 -6.403829496583224 2214 24 -5.713875652324479 2214 25 -3.5575373416916314 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20156.0 3 15.0 4 387.0 5 2289.0 6 3083.0 7 2476.0 8 1938.0 9 1547.0 10 1281.0 11 1175.0 12 1121.0 13 1073.0 14 1043.0 15 1060.0 16 1164.0 17 1249.0 18 1407.0 19 1649.0 20 2075.0 21 2715.0 22 3471.0 23 4253.0 24 5058.0 25 5788.0 26 6734.0 27 7513.0 28 8665.0 29 10214.0 30 12643.0 31 15709.0 32 20294.0 33 26118.0 34 34347.0 35 45740.0 36 62406.0 37 92626.0 38 148856.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.155961004380565 19.939981962378674 9.703273971426118 28.200783061814644 2 11.845357542718762 22.696298839966946 40.901806761315065 24.556536855999227 3 20.761570571633992 25.323088919650743 29.091416368884737 24.823924139830524 4 11.353215110913995 18.116965799432016 39.85893428140179 30.670884808252204 5 12.064673502891745 40.38749736219952 34.9427194918255 12.60510964308324 6 29.40210573918911 39.07748739768753 17.874377062624834 13.64602980049853 7 26.759965465234163 32.556002565030326 22.22423237168579 18.459799598049717 8 22.922847069951416 38.72085357639337 19.647591613282316 18.7087077403729 9 24.436661351869436 18.098317777100988 21.834321646751107 35.63069922427847 10 17.1337887132537 29.876439453544094 32.2124669193906 20.7773049138116 11 31.40963523694759 23.337362020446186 26.021504607363916 19.23149813524231 12 26.018876594131772 25.648603564704807 30.79327107883365 17.53924876232977 13 29.32502350993496 22.924063803996866 25.58327880458685 22.167633881481322 14 19.824020267368965 24.61390375862365 29.47606847393582 26.086007500071567 15 22.516841638537674 33.720285771497224 23.286157674924198 20.476714915040905 16 18.887331447717056 30.28094728975438 31.285556441548874 19.546164820979687 17 20.241724251986806 30.38913671187077 28.847787025200468 20.521352010941957 18 19.08130809540922 30.59159653649004 31.330175910977136 18.9969194571236 19 22.378202492267533 28.796595927270126 27.1676827633061 21.65751881715624 20 21.956526403112306 28.82959551461754 27.94031409119515 21.273563991075005 21 21.96425761974913 28.079901883662057 27.213875023241847 22.741965473346966 22 21.593741172600467 29.166621970972827 26.688513921814717 22.55112293461199 23 20.30829301781749 31.017023695869046 28.958340037687407 19.716343248626053 24 24.90935680878756 27.25141954031208 28.334131899251968 19.505091751648383 25 20.46419159792469 29.750705298048764 28.961379344868398 20.823723759158145 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 420.0 1 420.0 2 494.5 3 569.0 4 569.0 5 569.0 6 697.0 7 825.0 8 825.0 9 825.0 10 1231.0 11 1637.0 12 1637.0 13 1637.0 14 2224.0 15 2811.0 16 2811.0 17 2811.0 18 4665.0 19 6519.0 20 6519.0 21 6519.0 22 10529.0 23 14539.0 24 14539.0 25 14539.0 26 21429.5 27 28320.0 28 28320.0 29 28320.0 30 38279.0 31 48238.0 32 48238.0 33 48238.0 34 53452.5 35 58667.0 36 58667.0 37 58667.0 38 62182.5 39 65698.0 40 65698.0 41 65698.0 42 71281.5 43 76865.0 44 76865.0 45 76865.0 46 84024.0 47 91183.0 48 91183.0 49 91183.0 50 81290.0 51 71397.0 52 71397.0 53 71397.0 54 56167.5 55 40938.0 56 40938.0 57 40938.0 58 33040.0 59 25142.0 60 25142.0 61 25142.0 62 19617.5 63 14093.0 64 14093.0 65 14093.0 66 10531.5 67 6970.0 68 6970.0 69 6970.0 70 4961.5 71 2953.0 72 2953.0 73 2953.0 74 2010.5 75 1068.0 76 1068.0 77 1068.0 78 706.5 79 345.0 80 345.0 81 345.0 82 228.5 83 112.0 84 112.0 85 112.0 86 66.5 87 21.0 88 21.0 89 21.0 90 14.5 91 8.0 92 8.0 93 8.0 94 4.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09046408432825948 2 0.038617079476094954 3 0.036471686171867454 4 0.02967794070848038 5 0.028605244056366635 6 0.37526504546445977 7 0.18950974187342895 8 0.20059427394527102 9 0.2052426261044306 10 0.017520711984524564 11 0.0028605244056366633 12 0.0037544382823981206 13 0.0 14 0.0733009378944395 15 0.0017878277535229147 16 0.0028605244056366633 17 0.0041120038331027035 18 0.0037544382823981206 19 0.0014302622028183316 20 0.0017878277535229147 21 3.575655507045829E-4 22 0.0 23 0.0 24 3.575655507045829E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 559338.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.60762961515111 #Duplication Level Percentage of deduplicated Percentage of total 1 92.61284394205488 70.02237602376677 2 5.175174740048175 7.825653898784965 3 0.9469462285210497 2.147890791344513 4 0.3719276458116071 1.1248227075263637 5 0.20506912235960031 0.775239512443438 6 0.12290601760623149 0.5575579593987113 7 0.08625802825766452 0.4565235536287116 8 0.05832881414585938 0.3528082700664898 9 0.05181041699142574 0.3525536536284824 >10 0.3003449257571974 4.4634159126294985 >50 0.03169299189861044 1.6643900878047815 >100 0.0314546798980421 5.1864879013405645 >500 0.0028595163543655012 1.5899800510409352 >1k 0.0023829302953045843 3.4802996765957785 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGA 4239 0.7578601847183635 No Hit GCTTACTCTGCGTTGATACCACTGC 2919 0.5218669212533388 No Hit CTGTAGGACGTGGAATATGGCAAGA 1890 0.33789944541583083 No Hit CTGCTTACTCTGCGTTGATACCACT 1784 0.31894847122848796 No Hit GTCCTACAGTGGACATTTCTAAATT 1750 0.31286985686651003 No Hit GTATCAACGCAGAGTACTTTTTTTT 1649 0.2948127965559286 No Hit CTTTAGGACGTGAAATATGGCGAGG 1402 0.2506534510439126 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1308 0.23384787016079725 No Hit GTCCTACAGTGTGCATTTCTCATTT 1222 0.21847255148050018 No Hit GCGTTGATACCACTGCTTACTCTGC 1154 0.20631532275654432 No Hit CTGTAGGACCTGGAATATGGCGAGA 997 0.17824642702623458 No Hit GGTATCAACGCAGAGTACTTTTTTT 948 0.1694860710339723 No Hit GTATCAACGCAGAGTAAGCAGTGGT 912 0.16304989112128981 No Hit CTGAAGGACCTGGAATATGGCGAGA 798 0.14266865473112858 No Hit TATCAACGCAGAGTACTTTTTTTTT 785 0.14034447865154878 No Hit CCCATGTACTCTGCGTTGATACCAC 709 0.12675698772477464 No Hit GATATACACTGTTCTACAAATCCCG 667 0.11924811115997841 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 664 0.11871176283392151 No Hit ACGCAGAGTAAGCAGTGGTATCAAC 652 0.11656636952969404 No Hit GTAAGCAGTGGTATCAACGCAGAGT 598 0.10691209966067029 No Hit GTACATGGGAAGCAGTGGTATCAAC 566 0.10119105084939696 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTCCA 40 2.6693195E-4 16.695671 5 TCCAACG 130 0.0 15.301169 18 CCAACGA 90 2.3719622E-9 14.73446 19 TAGGACC 335 0.0 14.524166 4 TAAGACG 40 0.0051271054 14.310575 4 GGCGAGG 375 0.0 13.387309 19 CCAACGT 50 0.0015309465 13.261013 19 CTCCTAC 65 5.223492E-5 13.210952 1 ATCCCGT 95 7.543531E-8 12.974652 10 TGTAGGA 915 0.0 12.729681 2 GTCCTAG 90 5.108486E-7 12.721657 1 AGGACCT 555 0.0 12.376714 5 CGTTTCC 100 1.4798388E-7 12.321508 14 CCGTTTC 100 1.4798388E-7 12.321508 13 CTGTAGG 930 0.0 12.208687 1 GTAGGAC 930 0.0 12.1115465 3 GGACCTG 565 0.0 11.816752 6 CTAGACA 65 7.7291543E-4 11.74201 4 AATACTG 65 7.7291543E-4 11.74201 5 AGGACTG 90 7.0933056E-6 11.660469 5 >>END_MODULE