FastQCFastQC Report
Thu 26 May 2016
SRR1926516_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926516_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754775
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT20450.27094167135901426No Hit
CTGTAGGACGTGGAATATGGCAAGA14600.1934351296744063No Hit
GTCCTACAGTGTGCATTTCTCATTT11820.15660296114736177No Hit
GTCCTAAAGTGTGTATTTCTCATTT11770.1559405120731344No Hit
CTTTAGGACGTGAAATATGGCGAGG8870.1175184657679441No Hit
CTGTAGGACCTGGAATATGGCGAGA8210.10877413798814216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA5150.017.6792371
CTGTAGG10150.016.8851831
TGTAGGA10600.016.841182
AGGACGG503.894497E-516.69765
TAGGACC4650.015.7101914
TACCCCC400.002822741215.6539995
GTTTTAG604.4278902E-415.6209941
GTCCTAC10750.015.4445431
GTAGGAC11100.015.3152733
TCCTAAA5950.015.0013882
GTGTAGA902.7930855E-614.8771361
TAGGACA3100.014.5550294
GGACGGG652.2526507E-514.44588856
TCCTACA12300.014.40189552
GTGTAGG751.4769773E-414.2820511
CTAACAC550.00103947313.9589073
GTGTAAC1252.9813236E-813.9249991
TGTAGAA3850.013.9103782
TTCTAGG802.0611948E-413.732042
TGTTATA600.00673625213.732042
Produced by FastQC (version 0.11.5)