##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926516_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 754775 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.01182471597496 27.0 7.0 31.0 2.0 31.0 2 16.344167467125963 16.0 2.0 30.0 2.0 31.0 3 20.020831373588155 23.0 15.0 31.0 2.0 31.0 4 22.26758305455268 28.0 2.0 35.0 2.0 37.0 5 22.89858037163393 30.0 2.0 35.0 2.0 37.0 6 23.35696333344374 30.0 2.0 35.0 2.0 37.0 7 23.367824848464775 30.0 7.0 35.0 2.0 37.0 8 23.472595144248285 31.0 8.0 35.0 2.0 37.0 9 26.239387897055416 32.0 17.0 37.0 2.0 39.0 10 26.951454406942467 32.0 23.0 37.0 2.0 39.0 11 24.08680732668676 30.0 7.0 37.0 2.0 39.0 12 25.20680865158491 31.0 11.0 37.0 2.0 39.0 13 21.850882713391407 27.0 2.0 37.0 2.0 39.0 14 25.222307310125533 31.0 17.0 38.0 2.0 40.0 15 26.333229108012322 31.0 17.0 38.0 2.0 40.0 16 26.699657513828626 31.0 17.0 39.0 2.0 40.0 17 26.033330462720677 31.0 11.0 38.0 2.0 40.0 18 26.122799509787686 31.0 16.0 38.0 2.0 40.0 19 26.50999436918287 31.0 18.0 39.0 2.0 40.0 20 26.7485104832566 31.0 18.0 39.0 2.0 40.0 21 24.640091417972243 30.0 2.0 38.0 2.0 40.0 22 25.558142492795866 31.0 2.0 38.0 2.0 40.0 23 25.55624656354543 31.0 2.0 38.0 2.0 40.0 24 24.584660329237188 31.0 2.0 39.0 2.0 40.0 25 24.395588089165646 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.595783697592399 1101 2 10.471435511417504 1101 3 7.22761175315139 1101 4 8.663057718459523 1101 5 8.36081496332649 1101 6 7.850853471864408 1101 7 7.531007329413178 1101 8 5.850174347181305 1101 9 5.918903154710161 1101 10 5.228330712919714 1101 11 8.0006058762214 1101 12 5.840225723489954 1101 13 10.391590735855246 1101 14 7.646005601947451 1101 15 6.87505901477013 1101 16 6.492366339648875 1101 17 2.3687010475537917 1101 18 4.9482716563634135 1101 19 5.664301632268266 1101 20 3.446660351638073 1101 21 0.8268137070827315 1101 22 4.560539109595869 1101 23 5.90653942465805 1101 24 7.189073789352857 1101 25 7.66694081999151 1102 1 5.617461109315354 1102 2 10.980347007641946 1102 3 7.1232516261822845 1102 4 8.478062844541423 1102 5 8.333898104431753 1102 6 7.0704986878498985 1102 7 7.209769577791562 1102 8 7.392841585603261 1102 9 6.207284692929047 1102 10 5.577281344807886 1102 11 8.268889723148707 1102 12 5.679145993200727 1102 13 10.14815852828836 1102 14 7.65893170750531 1102 15 5.653343650325418 1102 16 6.1038635498773814 1102 17 6.896096012164108 1102 18 6.830162293377665 1102 19 5.4026324025606485 1102 20 5.911512188155491 1102 21 5.1524406663303814 1102 22 5.935484891421936 1102 23 6.498005484081169 1102 24 7.894454696669353 1102 25 7.713706459470227 1103 1 4.809454298739961 1103 2 9.187928279046787 1103 3 6.339771168831199 1103 4 7.949405935802158 1103 5 7.334202582945313 1103 6 6.479856043320037 1103 7 6.7159833752963 1103 8 6.098607556110256 1103 9 5.446726595992832 1103 10 4.485693598971935 1103 11 5.406908006474488 1103 12 4.125353774210037 1103 13 8.486998660017942 1103 14 6.599214336640735 1103 15 5.605370624685893 1103 16 5.457825911461448 1103 17 3.417172014872751 1103 18 1.8372194963649697 1103 19 3.9905310867263033 1103 20 4.548640785264652 1103 21 6.651181952155323 1103 22 2.3684466391773213 1103 23 4.611192326712061 1103 24 6.184684224752015 1103 25 1.977303251971776 1104 1 5.7953989289576455 1104 2 10.784593494586577 1104 3 7.292386340659249 1104 4 8.35168796132222 1104 5 8.168844698284989 1104 6 7.66025978082186 1104 7 7.430401012774755 1104 8 7.250981809244511 1104 9 6.046652102177124 1104 10 5.197328394226634 1104 11 8.121641175083177 1104 12 5.396275208144818 1104 13 9.684349740593444 1104 14 7.218122717550866 1104 15 6.236976761173274 1104 16 5.986866044411556 1104 17 6.350440739450846 1104 18 4.106972516152091 1104 19 4.867459711449474 1104 20 4.035721208930436 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2204 7 5.183098340545147 2204 8 5.1220757992492985 2204 9 3.6412802842239955 2204 10 3.080538478006961 2204 11 5.049514392852906 2204 12 4.8555935815057545 2204 13 2.903714044168744 2204 14 4.287775041362355 2204 15 4.2395532352120355 2204 16 4.080727370405949 2204 17 -0.9229506886866865 2204 18 2.5394109329160415 2204 19 3.467849457488544 2204 20 3.6903925389474885 2204 21 5.02043839532357 2204 22 4.643071934780551 2204 23 3.383542217735396 2204 24 4.928664414864546 2204 25 5.041682454404501 2205 1 5.7436580609840036 2205 2 10.142084216548966 2205 3 7.03800600320081 2205 4 7.495932128920138 2205 5 7.710165881642926 2205 6 7.2258970107010505 2205 7 7.2867144471892615 2205 8 6.950911447918408 2205 9 5.70756799509914 2205 10 4.995513255964326 2205 11 7.2696758866707185 2205 12 6.384901276786199 2205 13 9.499729438821536 2205 14 7.098567097612396 2205 15 6.029193556835704 2205 16 5.44541264614346 2205 17 6.158603802973786 2205 18 5.955247284028697 2205 19 4.824816378989908 2205 20 5.106509071831621 2205 21 -0.9838205949308794 2205 22 3.561263972389696 2205 23 4.85411631189011 2205 24 6.633951268713098 2205 25 7.012434432726504 2206 1 4.669921158289863 2206 2 9.237354655780397 2206 3 6.575422451907581 2206 4 2.3667089447457172 2206 5 6.407060262895897 2206 6 6.775805321111989 2206 7 6.74500244261154 2206 8 6.8054358898397 2206 9 5.354609259919851 2206 10 4.503777709465091 2206 11 4.025989965190348 2206 12 5.211554449088283 2206 13 8.809034242499933 2206 14 0.8128858800107004 2206 15 3.0510250282170617 2206 16 4.068324004613707 2206 17 5.206802226453529 2206 18 -0.03852480764286881 2206 19 1.6965720431311695 2206 20 3.2269271267958146 2206 21 3.989743742099325 2206 22 -1.3732697736594268 2206 23 -2.316951792973942 2206 24 1.8456871981909622 2206 25 3.511333932376189 2207 1 1.5457103045052598 2207 2 -13.862420287955537 2207 3 -6.374697250933201 2207 4 -4.5030253212614895 2207 5 1.0093202387544373 2207 6 1.7364597923043839 2207 7 2.773747769723734 2207 8 2.892742337721753 2207 9 0.5815923637677329 2207 10 0.20445517416408876 2207 11 -1.6485847611232103 2207 12 -2.770582248054634 2207 13 -0.1296374950595549 2207 14 -5.3556589340811165 2207 15 -1.6039615952559458 2207 16 -0.7745728328320745 2207 17 0.3749676419466539 2207 18 0.5355494683859696 2207 19 0.09011285373550137 2207 20 -0.442023201834548 2207 21 -4.067241812629067 2207 22 -4.515754323641605 2207 23 -1.4795909607995625 2207 24 0.3538442680628684 2207 25 0.7585508628521147 2208 1 4.124617315941801 2208 2 5.921993404688308 2208 3 5.649383403908146 2208 4 7.316445832718621 2208 5 2.919465676703826 2208 6 5.472902756432216 2208 7 5.976309358925469 2208 8 5.917644386246394 2208 9 4.277667227616437 2208 10 3.7312577404342306 2208 11 5.692715459382544 2208 12 5.152618557067207 2208 13 7.761517811316388 2208 14 1.9133263215121872 2208 15 3.6090591106633845 2208 16 4.086326320196246 2208 17 4.774910021792753 2208 18 -0.46108638394371937 2208 19 2.0921596508852573 2208 20 3.305495202900783 2208 21 5.856100535230599 2208 22 4.985100226706653 2208 23 0.9084607145593502 2208 24 2.6099190427507892 2208 25 3.9325759872453325 2209 1 3.437874016069525 2209 2 5.49074523454637 2209 3 5.557534112545856 2209 4 0.6518013849038127 2209 5 5.27068120245573 2209 6 5.716680127914376 2209 7 6.071721422127588 2209 8 4.54915500039537 2209 9 4.558348982035735 2209 10 3.477704829689081 2209 11 -0.3424522597823696 2209 12 3.2052656915442057 2209 13 2.137891628471138 2209 14 3.907929663839951 2209 15 4.333443458911692 2209 16 3.849563937022438 2209 17 1.4139672900306373 2209 18 3.4858820377755784 2209 19 4.148953445117822 2209 20 4.0448289706778375 2209 21 6.171796120182403 2209 22 4.890328162437868 2209 23 0.6113317653298189 2209 24 -2.8253346762707885 2209 25 0.4512645672088098 2210 1 -2.022356769011868 2210 2 -13.862420287955537 2210 3 -7.6798647111154015 2210 4 -1.7664712697391813 2210 5 -0.3817566095686651 2210 6 -1.1618070440646164 2210 7 -1.050774700726425 2210 8 -20.96800747100051 2210 9 -13.122918889890114 2210 10 -7.021935472384875 2210 11 -10.435695361354556 2210 12 -4.939974546904789 2210 13 -5.32492934318322 2210 14 -4.073105760079418 2210 15 -5.589044349316893 2210 16 -5.1047417736792084 2210 17 -4.813276845447145 2210 18 -4.897769239603942 2210 19 -10.224409387060502 2210 20 -6.907029348104903 2210 21 -5.064581450573254 2210 22 -9.795442259908953 2210 23 -7.747597961625111 2210 24 -10.175767217545674 2210 25 -7.892921159832881 2211 1 -0.16893960666809704 2211 2 4.663240676274633 2211 3 0.4086888944902327 2211 4 1.2429828343689806 2211 5 0.8748862007178317 2211 6 -0.17711836859737673 2211 7 -20.81538765491284 2211 8 -13.310931265712798 2211 9 -9.118463323933732 2211 10 -6.969307708079032 2211 11 -9.42757657574504 2211 12 -4.75251646755223 2211 13 -6.914696651343281 2211 14 -4.741712139634181 2211 15 -5.244260690568776 2211 16 -11.694306388598903 2211 17 -7.513581990907184 2211 18 -5.870478130343685 2211 19 -8.498133709512281 2211 20 -6.585399399625839 2211 21 -4.321315165706945 2211 22 -5.9202754986521064 2211 23 -7.804084268161169 2211 24 -22.12205473729291 2211 25 -21.954532534240464 2212 1 -1.3111682621544212 2212 2 -13.862420287955537 2212 3 -8.0978105458323 2212 4 -19.758572375584365 2212 5 -20.329623908146864 2212 6 -20.74632521152117 2212 7 -10.29946408803386 2212 8 -9.838230400936817 2212 9 -5.579419629468102 2212 10 -6.0110593994936785 2212 11 -6.164772096344141 2212 12 -3.340878643261238 2212 13 -6.259069168703187 2212 14 -4.210515073730921 2212 15 -4.175187575889378 2212 16 -4.42075288244245 2212 17 -4.67888495967421 2212 18 -3.7270273472905195 2212 19 -4.328704976760747 2212 20 -4.472561240518946 2212 21 -2.3443746202938236 2212 22 -3.5750857531320044 2212 23 -3.670909510110235 2212 24 -2.658678941114566 2212 25 -3.244341451437915 2213 1 -18.547633230307287 2213 2 -13.862420287955537 2213 3 -8.496921411260283 2213 4 -5.5193668488140695 2213 5 -20.329623908146864 2213 6 -10.483803622574712 2213 7 -20.81538765491284 2213 8 -10.570770856147316 2213 9 -7.997134116338721 2213 10 -6.982788880368897 2213 11 -5.960030503657432 2213 12 -4.8211350704418585 2213 13 -19.196967257875162 2213 14 -12.236948469768455 2213 15 -6.795189115992564 2213 16 -6.069532350666822 2213 17 -4.64816540146381 2213 18 -4.578874921077965 2213 19 -5.691377275804783 2213 20 -5.615429187671406 2213 21 -3.9988978483501008 2213 22 -4.852703103494477 2213 23 -4.609719285365177 2213 24 -3.5469252036141548 2213 25 -4.417399546330273 2214 1 -18.547633230307287 2214 2 -13.862420287955537 2214 3 -12.5782415599009 2214 4 -19.758572375584365 2214 5 -20.329623908146864 2214 6 -20.74632521152117 2214 7 -16.848752764541057 2214 8 -15.334070388064383 2214 9 -14.433709125682268 2214 10 -14.337350589768917 2214 11 -14.94020654103821 2214 12 -12.823693638434074 2214 13 -19.196967257875162 2214 14 -17.053866794811963 2214 15 -23.869455091812945 2214 16 -18.564556808535244 2214 17 -23.592897698527715 2214 18 -18.144285518554632 2214 19 -15.42993575159635 2214 20 -14.139808026964028 2214 21 -11.769747131572384 2214 22 -12.785184239406465 2214 23 -12.75068749095945 2214 24 -11.658756357883949 2214 25 -11.434990112886794 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 135829.0 3 6.0 4 678.0 5 2581.0 6 3341.0 7 3458.0 8 2837.0 9 2783.0 10 2463.0 11 2308.0 12 2270.0 13 2354.0 14 3546.0 15 7688.0 16 11308.0 17 12135.0 18 12317.0 19 14282.0 20 16943.0 21 19608.0 22 20402.0 23 21910.0 24 23153.0 25 24061.0 26 20032.0 27 15648.0 28 16221.0 29 19275.0 30 18490.0 31 21228.0 32 27474.0 33 35348.0 34 42883.0 35 49541.0 36 49238.0 37 52370.0 38 38766.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.01571811407104 18.474137614566637 12.923544743701129 25.586599527661193 2 13.044795960170221 19.57858522351997 44.784046351027094 22.59257246528271 3 20.015409855851885 23.57146566254661 28.561985373267966 27.851139108333534 4 10.959089054105057 16.26312123545963 39.10565413635346 33.67213557408186 5 11.701675221128882 37.97045064077861 37.59705335497154 12.730820783120969 6 31.124570138069085 37.96534352403572 18.112604772770045 12.797481565125151 7 27.293801729647825 30.377360422276272 22.454561277008377 19.874276571067522 8 22.928336375001795 41.16282745836267 20.419031743522684 15.489804423112849 9 25.814513069615302 15.403522548502027 20.255449762961508 38.52651461892116 10 14.029621747693863 30.105143231790716 35.52930333540234 20.335931685113085 11 33.62595591290384 21.46761406385105 26.470786403296987 18.43564361994812 12 24.63113465059591 23.797642182357368 33.14592858025454 18.425294586792177 13 29.417743046994037 21.890983694655716 25.48239541250182 23.208877845848427 14 18.60054276530446 24.079593020205024 27.788937759592912 29.530926454897603 15 21.140021334817494 35.231504595716125 22.80260928637491 20.825864783091475 16 19.664694064724635 28.32003100842905 31.757162510372538 20.25811241647377 17 19.404108514975693 29.691213668687062 30.840179721962762 20.064498094374482 18 19.163853474712194 27.683691015397606 34.385742729730566 18.766712780159637 19 22.911087791618687 26.444170044783277 27.359318294500106 23.285423869097926 20 22.70711602324478 28.72840734977336 29.478161839064576 19.086314787917285 21 20.795432673334506 26.834844282464175 26.954760147499286 25.414962896702036 22 21.217181843872375 30.802438400775124 28.487303360202393 19.493076395150112 23 20.41165538949678 29.567983360813283 29.613083197260053 20.407278052429888 24 22.635607389041212 26.97338213493906 30.665278998546736 19.725731477472983 25 21.084036077051753 28.551492310610733 30.41371341852484 19.95075819381267 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 771.0 1 771.0 2 1664.5 3 2558.0 4 2558.0 5 2558.0 6 3596.0 7 4634.0 8 4634.0 9 4634.0 10 5545.0 11 6456.0 12 6456.0 13 6456.0 14 8083.5 15 9711.0 16 9711.0 17 9711.0 18 14001.0 19 18291.0 20 18291.0 21 18291.0 22 25898.5 23 33506.0 24 33506.0 25 33506.0 26 44373.5 27 55241.0 28 55241.0 29 55241.0 30 67478.0 31 79715.0 32 79715.0 33 79715.0 34 85825.5 35 91936.0 36 91936.0 37 91936.0 38 93490.5 39 95045.0 40 95045.0 41 95045.0 42 93751.0 43 92457.0 44 92457.0 45 92457.0 46 89114.0 47 85771.0 48 85771.0 49 85771.0 50 77192.5 51 68614.0 52 68614.0 53 68614.0 54 57671.0 55 46728.0 56 46728.0 57 46728.0 58 38530.0 59 30332.0 60 30332.0 61 30332.0 62 23970.5 63 17609.0 64 17609.0 65 17609.0 66 13353.5 67 9098.0 68 9098.0 69 9098.0 70 6567.0 71 4036.0 72 4036.0 73 4036.0 74 2776.0 75 1516.0 76 1516.0 77 1516.0 78 1024.5 79 533.0 80 533.0 81 533.0 82 350.5 83 168.0 84 168.0 85 168.0 86 104.5 87 41.0 88 41.0 89 41.0 90 23.5 91 6.0 92 6.0 93 6.0 94 3.0 95 0.0 96 0.0 97 0.0 98 1.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.382398728097778 2 36.612102944586134 3 5.596899738332615 4 13.642343744824617 5 13.707131264284058 6 14.817130933059522 7 9.41366632440131 8 7.8016627471763105 9 0.11778344539763506 10 1.3854459938392236 11 5.61319598555861 12 5.642873704083998 13 16.17687390281872 14 1.5294624225762645 15 2.875161471961843 16 2.924182703454672 17 2.837931833990262 18 1.451823391076811 19 1.394322811433871 20 0.29969196118048425 21 4.319432943592461 22 1.545493690172568 23 0.11818091484217151 24 9.10628995395979 25 0.12507038521413666 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 754775.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.733990020806 #Duplication Level Percentage of deduplicated Percentage of total 1 96.63538927707808 85.74843667768948 2 2.230919824138873 3.95916834824714 3 0.4649917811844755 1.2378172821414015 4 0.19106283093078427 0.6781506933263662 5 0.1172177108572772 0.5200597592735678 6 0.0718704439313125 0.3826410752755189 7 0.05176634081047003 0.32154037782229233 8 0.03171607628715432 0.22514351974107782 9 0.025731496319963017 0.20549325039084043 >10 0.15304607232720938 2.5579783162738203 >50 0.014552674417672872 0.9127998058693138 >100 0.010483688070141358 2.046015457987991 >500 6.258918233163027E-4 0.3905701326909083 >1k 6.258918233163027E-4 0.8141853032703471 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2045 0.27094167135901426 No Hit CTGTAGGACGTGGAATATGGCAAGA 1460 0.1934351296744063 No Hit GTCCTACAGTGTGCATTTCTCATTT 1182 0.15660296114736177 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1177 0.1559405120731344 No Hit CTTTAGGACGTGAAATATGGCGAGG 887 0.1175184657679441 No Hit CTGTAGGACCTGGAATATGGCGAGA 821 0.10877413798814216 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.3248981484548374E-4 0.0 9 0.0 0.0 0.0 1.3248981484548374E-4 1.3248981484548374E-4 10 0.0 0.0 0.0 1.3248981484548374E-4 1.3248981484548374E-4 11 0.0 0.0 0.0 1.3248981484548374E-4 1.3248981484548374E-4 12 0.0 0.0 0.0 1.3248981484548374E-4 1.3248981484548374E-4 13 0.0 0.0 0.0 1.3248981484548374E-4 1.3248981484548374E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAA 515 0.0 17.679237 1 CTGTAGG 1015 0.0 16.885183 1 TGTAGGA 1060 0.0 16.84118 2 AGGACGG 50 3.894497E-5 16.6976 5 TAGGACC 465 0.0 15.710191 4 TACCCCC 40 0.0028227412 15.653999 5 GTTTTAG 60 4.4278902E-4 15.620994 1 GTCCTAC 1075 0.0 15.444543 1 GTAGGAC 1110 0.0 15.315273 3 TCCTAAA 595 0.0 15.001388 2 GTGTAGA 90 2.7930855E-6 14.877136 1 TAGGACA 310 0.0 14.555029 4 GGACGGG 65 2.2526507E-5 14.4458885 6 TCCTACA 1230 0.0 14.4018955 2 GTGTAGG 75 1.4769773E-4 14.282051 1 CTAACAC 55 0.001039473 13.958907 3 GTGTAAC 125 2.9813236E-8 13.924999 1 TGTAGAA 385 0.0 13.910378 2 TTCTAGG 80 2.0611948E-4 13.73204 2 TGTTATA 60 0.006736252 13.73204 2 >>END_MODULE