FastQCFastQC Report
Thu 26 May 2016
SRR1926516_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926516_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754775
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT40670.5388360769765824No Hit
CTGTAGGACGTGGAATATGGCAAGA40220.5328740353085356No Hit
GTCCTACAGTGTGCATTTCTCATTT27370.36262462323208905No Hit
CTTTAGGACGTGAAATATGGCGAGG26640.35295286674836873No Hit
GTCCTAAAGTGTGTATTTCTCATTT26080.3455334371170216No Hit
CTGTAGGACCTGGAATATGGCGAGA22100.29280249080851906No Hit
GTATCAACGCAGAGTACTTTTTTTT16160.21410354079030172No Hit
CTGAAGGACCTGGAATATGGCGAGA15860.21012884634493723No Hit
GATATACACTGTTCTACAAATCCCG13880.18389586300553146No Hit
GTGTATATCAATGAGTTACAATGAA11330.15011096021993312No Hit
GTGTATATCAATGAGTTACAATGAG9700.12851512040011925No Hit
GGTATCAACGCAGAGTACTTTTTTT9590.12705773243681892No Hit
ACCTGGAATATGGCGAGAAAACTGA9080.12030075187969924No Hit
GAATATGGCAAGAAAACTGAAAATC9010.11937332317578087No Hit
GTCCTTCAGTGTGCATTTCTCATTT8960.11871087410155344No Hit
CTGTAGGACATGGAATATGGCAAGA8910.11804842502732603No Hit
GTTCTACAGTGTGGTTTTTATCATT8440.11182140372958829No Hit
GATATACACTGTTCTACAATGCCGG8430.1116889139147428No Hit
ACAGTGGACATTTCTAAATTTTCCA8040.10652181113576893No Hit
CTGTAGAACATATTAGATGAGTGAG8000.10599185187638699No Hit
GACCTGGAATATGGCGAGAAAACTG8000.10599185187638699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGAG250.00589456319.074456
AAGGCGT402.6776397E-416.6901446
GTCGAGG653.4731256E-614.57155719
TATGTCG851.7762432E-814.48872116
TAGGACC6650.014.3426484
GTGCCGT400.005140603514.3058386
ATACGGC552.005181E-413.77948415
CCTCTAA706.968292E-613.6236981
GGCGAGG6200.013.59620219
TCTAGGA1550.013.5457422
TAGGACT1850.013.4045614
TGTAGGA19700.013.3707172
CGGTTTC802.0425487E-613.03541913
CTGTAGG19500.013.0088851
GATATAC5800.012.9894921
GTAGGAC19350.012.9670663
TAGGCTG603.9527487E-412.7171485
CTAGGCT751.4170144E-512.7171484
GAACCAT603.9549748E-412.7162996
TGTCGAG905.5836244E-712.63035618
Produced by FastQC (version 0.11.5)