FastQCFastQC Report
Thu 26 May 2016
SRR1926515_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926515_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513157
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12150.23676964359835295No Hit
CTGTAGGACGTGGAATATGGCAAGA8890.1732413277028278No Hit
GTCCTACAGTGTGCATTTCTCATTT7800.15200026502610312No Hit
GTCCTAAAGTGTGTATTTCTCATTT6600.1286156088682411No Hit
GTACATGGGAAGCAGTGGTATCAAC6010.11711815292395895No Hit
CCCATGTACTCTGCGTTGATACCAC6000.11692328078931011No Hit
CTGTAGGACCTGGAATATGGCGAGA5910.11516943157747044No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG405.351993E-420.055581
TAAGACT401.0715792E-418.8080734
TCTAAGA459.885222E-418.3071212
GGACTGA350.001148174917.9054166
ATCCTAC450.001181552417.8271831
GATATAC2200.017.6246011
TCTAGGA653.504541E-516.898882
TGTAGGA6050.016.7941362
CTGTAGG5850.016.6844141
TAGGACC3350.016.682644
TCCCGTT1000.016.56049211
GTCCTAA2750.016.5306591
GCAGACC400.00201603516.4612483
GTCCTAC7750.016.2169841
TCCTACA8200.016.0745452
CTAGGAC505.0202804E-415.3638313
CCAACGA1001.8189894E-1215.29526419
TCCAACG1400.015.18219418
CTACACC505.882949E-415.0464574
TCCTAGG550.003780397414.9785532
Produced by FastQC (version 0.11.5)