FastQCFastQC Report
Thu 26 May 2016
SRR1926515_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926515_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513157
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA27020.5265445078211931No Hit
GTCCTACAGTGGACATTTCTAAATT26400.5144624354729644No Hit
GTCCTACAGTGTGCATTTCTCATTT18040.3515493309065257No Hit
GTACATGGGAAGCAGTGGTATCAAC17630.3435595733859228No Hit
CTGTAGGACCTGGAATATGGCGAGA16760.32660569767147285No Hit
CCCATGTACTCTGCGTTGATACCAC16320.31803132374692344No Hit
GTCCTAAAGTGTGTATTTCTCATTT16220.31608260240043495No Hit
CTTTAGGACGTGAAATATGGCGAGG15930.3104313104956183No Hit
GTATCAACGCAGAGTACTTTTTTTT12180.2373542600022995No Hit
GATATACACTGTTCTACAAATCCCG10250.1997439380150714No Hit
CTGAAGGACCTGGAATATGGCGAGA10180.19837983307252946No Hit
GGTATCAACGCAGAGTACTTTTTTT8380.16330284883573642No Hit
GAGTACATGGGAAGCAGTGGTATCA7710.15024641581426346No Hit
GTGTATATCAATGAGTTACAATGAA7510.14634897312128647No Hit
GTGTATATCAATGAGTTACAATGAG6850.13348741223446237No Hit
GTTCTACAGTGTGGTTTTTATCATT6720.13095407448402732No Hit
CTGTAGGACATGGAATATGGCAAGA6600.1286156088682411No Hit
ACCTGGAATATGGCGAGAAAACTGA6410.12491303830991295No Hit
GATATACACTGTTCTACAATGCCGG6350.12374380550201984No Hit
CATGTACTCTGCGTTGATACCACTG6350.12374380550201984No Hit
GTCCTTCAGTGTGCATTTCTCATTT5970.11633866438536355No Hit
CTGTAGAACATATTAGATGAGTGAG5890.11477968730817273No Hit
TATCAACGCAGAGTACTTTTTTTTT5780.11263609382703539No Hit
GAATATGGCAAGAAAACTGAAAATC5530.10776429046081414No Hit
GACCTGGAATATGGCGAGAAAACTG5430.10581556911432564No Hit
ACAGTGGACATTTCTAAATTTTCCA5340.104061719902486No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5320.10367197563318829No Hit
ACGCAGAGTACATGGGAAGCAGTGG5290.10308735922924173No Hit
TTGTAGAACAGTGTATATCAATGAG5230.10191812642134862No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAC350.002110307816.3510483
TAGGACC5250.014.7144994
CTAGGAC851.61981E-814.5876983
GTATAAC400.005154540814.2987511
ACTGGGC400.00526622814.2526398
CCGGTTT602.6118885E-514.22067512
CCAACGA1152.5465852E-1114.00501919
GGACCTA551.892342E-413.8722556
TAGTACA551.892342E-413.8722554
CGGTTTC707.395549E-613.54350113
GTAGGAC13850.013.4979763
TAATACT500.001453714613.3520454
CTGTAGG14300.013.3321681
TGTAGGA14550.013.3100792
AAGTGTA655.419169E-513.1588547
TATGTCG655.5803448E-513.11782416
AGGACCT8350.013.0208145
GTCCTAT1102.59206E-912.9988641
TAGGACA3900.012.9607774
GTAGGAA751.4145271E-512.7174813
Produced by FastQC (version 0.11.5)