##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926515_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 513157 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.025676352461332 31.0 28.0 31.0 16.0 34.0 2 28.54240125341757 31.0 28.0 33.0 22.0 34.0 3 28.877546248029354 31.0 28.0 33.0 23.0 34.0 4 32.573169614757276 35.0 32.0 37.0 26.0 37.0 5 32.206527437022196 35.0 32.0 37.0 23.0 37.0 6 32.261434609680855 35.0 33.0 37.0 23.0 37.0 7 32.071558996564406 35.0 32.0 37.0 19.0 37.0 8 32.03302108321625 35.0 32.0 37.0 19.0 37.0 9 33.214536681756265 37.0 33.0 39.0 17.0 39.0 10 33.100509200887835 37.0 33.0 39.0 17.0 39.0 11 33.31924732586713 37.0 33.0 39.0 19.0 39.0 12 33.1176482051302 37.0 33.0 39.0 17.0 39.0 13 33.260668762191685 37.0 33.0 39.0 18.0 39.0 14 33.847027323022 38.0 33.0 40.0 17.0 41.0 15 33.991926447461495 38.0 33.0 40.0 17.0 41.0 16 33.96398762951689 38.0 33.0 40.0 17.0 41.0 17 33.900270287650756 38.0 33.0 40.0 17.0 41.0 18 33.88141445990213 38.0 33.0 40.0 17.0 41.0 19 33.91027697176498 39.0 33.0 40.0 17.0 41.0 20 33.901277776586895 39.0 33.0 40.0 16.0 41.0 21 33.78643378147429 39.0 33.0 40.0 11.0 41.0 22 33.72110679577595 39.0 33.0 40.0 10.0 41.0 23 33.37698599064224 39.0 33.0 40.0 2.0 41.0 24 33.44684765091385 39.0 33.0 40.0 2.0 41.0 25 33.22482398174438 38.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1797041673895272 1101 2 1.4281394176485485 1101 3 1.5299914275625 1101 4 1.2436729197908392 1101 5 1.2702111579293742 1101 6 0.7757153600601967 1101 7 1.154670612407223 1101 8 1.1633611974390021 1101 9 1.4379392908525617 1101 10 1.5339565251309324 1101 11 1.2739561385036566 1101 12 1.5084087595001563 1101 13 1.3114409814769559 1101 14 1.690462454126397 1101 15 1.5563304015884754 1101 16 1.6122710962939237 1101 17 1.5097353626929646 1101 18 1.5693730216562543 1101 19 1.8102921005674375 1101 20 1.8332877981797964 1101 21 1.755788520051908 1101 22 1.5782704448908405 1101 23 1.8366996226722776 1101 24 1.9396843234693506 1101 25 1.9420886726774498 1102 1 0.4246434013289857 1102 2 0.545185515585267 1102 3 0.4166676623051515 1102 4 -0.04690998670974267 1102 5 0.1801365006025506 1102 6 -0.057040377493628114 1102 7 0.28914992034565046 1102 8 0.3730706000487203 1102 9 0.8975018805532713 1102 10 0.784287677118499 1102 11 0.42668106509719905 1102 12 0.4327283507940791 1102 13 0.30188826708402416 1102 14 0.6734426903437623 1102 15 0.5374093118811771 1102 16 0.46203738067644906 1102 17 0.0863967186385608 1102 18 0.2450191959620227 1102 19 0.1915751005542745 1102 20 0.1928622382498446 1102 21 0.46959288362123885 1102 22 0.3232992149541758 1102 23 0.5134268140030755 1102 24 0.31651685141647334 1102 25 0.16852289654409702 1103 1 0.6445272640855215 1103 2 0.5825562532423731 1103 3 0.3663401977031455 1103 4 0.5830231946022337 1103 5 0.5566889744849277 1103 6 0.3953340723128136 1103 7 0.5320477832082489 1103 8 0.48229131384200485 1103 9 0.6861864539212519 1103 10 1.03106473603561 1103 11 0.6733600172104772 1103 12 0.7589573373773106 1103 13 0.663630590889511 1103 14 1.1341522411736946 1103 15 1.058694511011474 1103 16 0.8189830772347619 1103 17 0.8918694873716575 1103 18 0.8084876692080343 1103 19 1.133762351972841 1103 20 1.1901324827213955 1103 21 1.2695186406345513 1103 22 0.9631239484748875 1103 23 1.2955223223023822 1103 24 1.1085940641226273 1103 25 1.1173880394618436 1104 1 0.3222808564052819 1104 2 0.5530436450571798 1104 3 0.3556840214067414 1104 4 0.056702893709740465 1104 5 0.4793385116133635 1104 6 -0.1158545881129669 1104 7 0.21481322005303127 1104 8 0.3355893387155078 1104 9 0.6339835281157775 1104 10 0.6484541092108813 1104 11 0.3875082504021563 1104 12 0.5685462076797663 1104 13 0.3855167276002618 1104 14 0.7443530786023942 1104 15 0.6044037279221328 1104 16 0.5389860383660121 1104 17 0.35814895582262096 1104 18 0.5024534551546083 1104 19 0.5720266615097671 1104 20 0.48620534733016285 1104 21 0.5457350421847309 1104 22 0.5427926654219064 1104 23 0.9274004587637776 1104 24 0.773310886465147 1104 25 0.8438808132625866 1105 1 1.0995295704767862 1105 2 1.1219801679571475 1105 3 1.0243984725224777 1105 4 0.8135931295107497 1105 5 1.109364388354031 1105 6 0.5266971495512962 1105 7 0.6350729997527651 1105 8 0.5922587680985814 1105 9 1.1353292451672203 1105 10 1.1084198177843732 1105 11 1.0065887087178282 1105 12 0.9353860186340412 1105 13 0.9161227747857694 1105 14 1.4555914293239667 1105 15 1.3678675416095984 1105 16 1.3617499779574302 1105 17 1.1820340099486657 1105 18 1.230707186403464 1105 19 1.007475898177546 1105 20 1.1753984865141263 1105 21 1.2983331555235864 1105 22 1.274905763861078 1105 23 1.433238226151495 1105 24 1.2598113261799284 1105 25 1.6823795826938692 1106 1 0.6559541514823728 1106 2 0.584550000359382 1106 3 0.4311023831370022 1106 4 0.31862106247164235 1106 5 0.58757667885682 1106 6 -0.7593363700017797 1106 7 -0.623027558906454 1106 8 -0.4479646955885741 1106 9 -0.3685813693579192 1106 10 -0.3128092324949634 1106 11 -0.6738582186564699 1106 12 -0.432211746729088 1106 13 -0.7308045995159063 1106 14 -0.4315594086648602 1106 15 -0.515931341071969 1106 16 -0.44830588796435933 1106 17 -0.5107034202189311 1106 18 -0.5268347130378785 1106 19 -0.39587605712971197 1106 20 -0.49834453024565306 1106 21 -0.4390970120741784 1106 22 -0.24017803502160717 1106 23 -0.06396847775912562 1106 24 -0.4418646514737006 1106 25 -0.45505058490390127 1107 1 0.8385710418385734 1107 2 1.0485192525444376 1107 3 1.0138966647332772 1107 4 0.8316020360292598 1107 5 0.8594336818071824 1107 6 0.7410005501660066 1107 7 0.9990083751384944 1107 8 1.2048707925145123 1107 9 1.4293626168942595 1107 10 0.8164128945984714 1107 11 0.806641679713131 1107 12 1.3079544343701244 1107 13 1.017032520832963 1107 14 1.3299282139845516 1107 15 1.017413900469613 1107 16 1.0800526887173163 1107 17 1.1208328002389578 1107 18 1.1392496833083499 1107 19 0.9815681539412253 1107 20 0.9599161732830765 1107 21 1.2206530387410197 1107 22 1.372431525627107 1107 23 1.2057351926381017 1107 24 1.2714760320985121 1107 25 1.1591099172118575 1108 1 1.1377867488818048 1108 2 1.0528198644758966 1108 3 0.9617139808047845 1108 4 0.6125392396324827 1108 5 -0.1924396661945309 1108 6 0.39804329870598565 1108 7 0.7345887471119426 1108 8 0.9329994184852453 1108 9 1.413936744374361 1108 10 1.068858694610988 1108 11 0.702149929473606 1108 12 0.7223502302902602 1108 13 0.7244404542763903 1108 14 1.0961930247961433 1108 15 0.8735907491429558 1108 16 0.9544093422081161 1108 17 1.0642483984729765 1108 18 1.1142596735347823 1108 19 1.3433142529383488 1108 20 1.2498806899246304 1108 21 1.2396019706293444 1108 22 1.2310168614190786 1108 23 1.3324148746840976 1108 24 1.3736375319324097 1108 25 1.2221614860385017 1109 1 0.9796319684562818 1109 2 0.018167009954044744 1109 3 0.775067628219567 1109 4 1.0255034146053887 1109 5 -0.5074804910606829 1109 6 0.371882660099935 1109 7 0.7743809211835924 1109 8 0.8437158117343913 1109 9 1.0890813212656596 1109 10 -1.1587922393718202 1109 11 0.24937212189230706 1109 12 0.5650457443831485 1109 13 0.42835288198794785 1109 14 1.2689717980115987 1109 15 1.1025908886234461 1109 16 1.3010880971838148 1109 17 1.214514484328582 1109 18 1.4019918280791615 1109 19 1.0294214597879119 1109 20 0.9579372975445537 1109 21 1.0404426088062237 1109 22 1.206014983797111 1109 23 1.5709291928927414 1109 24 1.534793825184778 1109 25 1.8120942135258176 1110 1 0.7415636766887062 1110 2 -0.26914395038794225 1110 3 0.43369968183968766 1110 4 0.764861779262084 1110 5 1.1135576657769946 1110 6 0.4929589327275181 1110 7 0.9185758863809426 1110 8 1.0313569134566407 1110 9 1.195867104662426 1110 10 1.1559005180416975 1110 11 1.106734040113409 1110 12 1.1196128956090163 1110 13 0.9626688208384877 1110 14 1.2576660683287813 1110 15 1.073526132828384 1110 16 0.9126119664642474 1110 17 1.2028217327790287 1110 18 1.2193966705436736 1110 19 1.1206823887576718 1110 20 1.1468802000912746 1110 21 1.1398192690420714 1110 22 1.384493636022782 1110 23 1.4647298062637546 1110 24 1.3862976396957478 1110 25 1.496671392136406 1111 1 0.010796174039089834 1111 2 0.42974757269739783 1111 3 0.34663904862819805 1111 4 0.1689523603799188 1111 5 0.397893623615758 1111 6 0.1929010508857658 1111 7 0.4885904493466029 1111 8 0.4871565947100578 1111 9 0.6987757332856575 1111 10 0.5466994196082098 1111 11 0.32858800343376515 1111 12 0.39158354766514236 1111 13 0.11215462392922149 1111 14 0.3924858285023731 1111 15 0.5909303304512576 1111 16 0.44665256832968225 1111 17 0.4175382571197943 1111 18 0.4127286295136372 1111 19 0.4404782429377221 1111 20 0.2049721608584889 1111 21 0.4054163109892315 1111 22 0.2553087656490334 1111 23 0.6400708201739036 1111 24 0.4329969210622906 1111 25 0.551626104220496 1112 1 0.3115538622338434 1112 2 0.18496595510350744 1112 3 0.4359632942698717 1112 4 0.5587869324249937 1112 5 0.6806814290600016 1112 6 0.3258370831265225 1112 7 0.5967027744332754 1112 8 0.8322300992177531 1112 9 0.35419695236067383 1112 10 0.863737319240208 1112 11 0.4912911730897065 1112 12 0.6835503783517041 1112 13 0.2628626494874027 1112 14 0.7596658660252515 1112 15 0.7116010234067787 1112 16 0.7367965328922494 1112 17 0.5359103881987082 1112 18 0.43660094516251036 1112 19 0.7030622983772048 1112 20 0.2782749735523353 1112 21 0.3748918659358935 1112 22 0.39880524359768543 1112 23 0.6349941951020668 1112 24 0.5214801366133557 1112 25 0.5752130024177973 1113 1 0.07767948640671207 1113 2 0.18161432752757634 1113 3 -1.2543955286707735 1113 4 -0.6820298026365634 1113 5 -1.1517816766240045 1113 6 -0.15903263295128056 1113 7 0.08507842362866569 1113 8 0.08443347114137367 1113 9 0.5061962517301097 1113 10 0.6702492911260194 1113 11 0.29279308036180396 1113 12 0.7260122051068905 1113 13 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0.5835716237714195 2202 19 0.5659121451209401 2202 20 0.4009305062488977 2202 21 0.45108302771566144 2202 22 0.33825291482225595 2202 23 0.570674553167315 2202 24 0.4985984135759125 2202 25 0.36748918953821175 2203 1 -0.40073732994580524 2203 2 -0.2620175817059547 2203 3 -0.45339907565697146 2203 4 -0.6841329835555996 2203 5 -0.593682724803898 2203 6 -0.6783477917698626 2203 7 -0.28878187968413016 2203 8 -0.3419921444653653 2203 9 0.3499839100371034 2203 10 -0.031718604101463654 2203 11 -0.2727409002207182 2203 12 0.007136440572210745 2203 13 0.020384023199412127 2203 14 0.09162167803852839 2203 15 0.16551777122246136 2203 16 -0.02354694876270713 2203 17 -0.12389013276456495 2203 18 0.02725579760542729 2203 19 -0.00907061968776901 2203 20 0.19473306712720984 2203 21 0.3071827362020869 2203 22 0.24497889981020649 2203 23 -0.009467149798688013 2203 24 0.16352176538812557 2203 25 0.2938968592628868 2204 1 0.41017601900033185 2204 2 0.4302259780130129 2204 3 0.19758921474115354 2204 4 0.07048059958257369 2204 5 0.32781610423591445 2204 6 0.039891084108354846 2204 7 0.2844265512292168 2204 8 0.2645652325838128 2204 9 0.7256373058216496 2204 10 0.6490870165074369 2204 11 0.2484019569221374 2204 12 0.2349678223052294 2204 13 0.3040748897099519 2204 14 0.5035467365865358 2204 15 0.6186512606838193 2204 16 0.580987009082726 2204 17 0.3875759093900015 2204 18 0.42223472032205933 2204 19 0.4091869915534474 2204 20 0.48595554956281006 2204 21 0.47748718585080496 2204 22 0.5826088763910064 2204 23 0.8830387050022495 2204 24 0.5787067770977288 2204 25 0.510580700012305 2205 1 0.549706653745961 2205 2 0.33076842564256026 2205 3 0.3600929161114479 2205 4 0.05431427417700263 2205 5 0.10640170826804507 2205 6 -0.38468853472925346 2205 7 -0.06052855981188898 2205 8 -0.012328766471156882 2205 9 0.32131410531763294 2205 10 0.029202310254483166 2205 11 0.03376406119052433 2205 12 -0.10329143112050332 2205 13 0.1173632815826906 2205 14 0.4611299780418818 2205 15 0.35368732935604896 2205 16 0.21015191694223034 2205 17 0.2961359975928701 2205 18 0.36637206478575024 2205 19 0.3597379444594182 2205 20 0.3722645746061204 2205 21 0.5194402493253634 2205 22 0.5907336204451852 2205 23 0.9345113778641192 2205 24 1.0101750398162679 2205 25 1.0146777231655548 2206 1 0.5648743622325902 2206 2 0.6251756497854437 2206 3 0.5302356667574095 2206 4 0.37443808629717523 2206 5 0.7506794990467895 2206 6 0.7375916155244227 2206 7 1.026183350228905 2206 8 0.7633148418367028 2206 9 0.9939248956934819 2206 10 0.7813633528625488 2206 11 0.6528608503263271 2206 12 0.6923582568160143 2206 13 0.5317297818637527 2206 14 0.9602819663458391 2206 15 0.7988150618716929 2206 16 0.9804143301205528 2206 17 0.9196634367423044 2206 18 0.903018715320492 2206 19 0.8809744251264391 2206 20 0.9175204135828139 2206 21 1.0305850289267653 2206 22 1.1050171289699335 2206 23 1.3349924405446103 2206 24 1.2638246264256452 2206 25 1.388296236117334 2207 1 -0.8098843655539341 2207 2 0.2237120736748608 2207 3 0.45876067509392726 2207 4 0.6516851125334711 2207 5 0.5151162790333075 2207 6 0.5242400432693302 2207 7 0.5487138545821537 2207 8 0.5294627377482826 2207 9 0.7703638273535063 2207 10 0.5026169704244836 2207 11 0.3757574312587053 2207 12 0.13557865974365058 2207 13 0.08576502160253341 2207 14 -4.727937134381204 2207 15 -1.4922961411912823 2207 16 -0.5505597889468277 2207 17 -0.43773221074347646 2207 18 0.029019401714080573 2207 19 0.29724292671950536 2207 20 0.35320995922433696 2207 21 0.49393720968681265 2207 22 0.13936845052099045 2207 23 0.5398955300459463 2207 24 0.06647795141839907 2207 25 0.11559960991218787 2208 1 0.7307344182557998 2208 2 1.189809039902265 2208 3 1.2537095808028873 2208 4 1.045830374671766 2208 5 1.1799519813880792 2208 6 1.2478067037764191 2208 7 1.3906216807468255 2208 8 1.4316196765112252 2208 9 1.709514457985442 2208 10 1.6512982775590856 2208 11 1.569821531918258 2208 12 1.7538721188772755 2208 13 1.7573871279602997 2208 14 1.8014663891381133 2208 15 1.5042774434044617 2208 16 1.8486314948900784 2208 17 1.5358842698677435 2208 18 1.662778228930712 2208 19 1.620129135186147 2208 20 1.698787564453454 2208 21 1.853178204929442 2208 22 1.6874874245529412 2208 23 1.9704281884829413 2208 24 1.9153557611213046 2208 25 1.7812082787310857 2209 1 -0.5043761966798677 2209 2 -0.3575918094056405 2209 3 0.2828783377656343 2209 4 0.17097021842360505 2209 5 0.12097383587520483 2209 6 0.16155578816785265 2209 7 0.5441848406621261 2209 8 0.500542920839159 2209 9 0.6370088583472153 2209 10 0.12776566540733825 2209 11 0.4962395770027115 2209 12 0.4948426286268912 2209 13 0.515560480041529 2209 14 0.6381324198837177 2209 15 0.6881622616134777 2209 16 0.45190860809109523 2209 17 0.7393589896503059 2209 18 0.6231780113043897 2209 19 0.5002945217672661 2209 20 0.3532775204335863 2209 21 0.6432076692719377 2209 22 0.6003132457743305 2209 23 0.7466581658017546 2209 24 0.5548815524308566 2209 25 0.5371924136782482 2210 1 -0.7451303432625522 2210 2 -0.6430337164640711 2210 3 0.07209786003240737 2210 4 0.33898488026076734 2210 5 0.3250160370880195 2210 6 0.2779290226680331 2210 7 0.3354320909304107 2210 8 0.4300847894077151 2210 9 0.7359673377169003 2210 10 0.8637480875163348 2210 11 0.5592214969590685 2210 12 0.36228962858356084 2210 13 0.26442808762836734 2210 14 0.7527876296535183 2210 15 0.36805398017349233 2210 16 0.5237138850269929 2210 17 0.5543820198482905 2210 18 0.749497436454341 2210 19 0.6625803103380719 2210 20 0.6238383764891111 2210 21 0.6974771136668139 2210 22 0.4562553427331224 2210 23 0.6415413069831146 2210 24 0.29726578330890874 2210 25 0.4014009115281638 2211 1 -1.2081136959521608 2211 2 -0.03652781428570151 2211 3 0.34800361714155414 2211 4 0.45510470359991473 2211 5 0.4217050857328317 2211 6 0.28455586157127044 2211 7 0.4274303852015464 2211 8 0.17254989669449827 2211 9 0.6376479742663861 2211 10 0.4699824990878625 2211 11 0.0668156925447434 2211 12 0.13521653042325 2211 13 0.2062865916602803 2211 14 0.4432526875783651 2211 15 0.37746839687318356 2211 16 -0.003382385816422584 2211 17 0.2049201334273434 2211 18 0.051027519121859655 2211 19 0.14311731327373423 2211 20 0.24121075876433906 2211 21 0.2720129401431848 2211 22 0.338565535834249 2211 23 0.5716201352406216 2211 24 0.4437829564609572 2211 25 0.3586740489037865 2212 1 1.0155919951355656 2212 2 0.3551354907740354 2212 3 0.5997396953510297 2212 4 0.42824659229056294 2212 5 0.4146308208418148 2212 6 0.4530007957424331 2212 7 0.6707507143051217 2212 8 0.36748203735676555 2212 9 1.0090035636937031 2212 10 0.46351061610968713 2212 11 0.7086976148604904 2212 12 0.7975916207805369 2212 13 0.7955379324676599 2212 14 0.8815606577002271 2212 15 0.9370311082144767 2212 16 0.34739204720006 2212 17 0.6685782295756155 2212 18 0.7383845380217551 2212 19 0.78806799917691 2212 20 0.5132369549323812 2212 21 0.719741080448081 2212 22 0.6412008551778783 2212 23 0.9653721488206131 2212 24 1.0975785039787382 2212 25 0.47375471393341684 2213 1 0.0483578371821487 2213 2 -0.20857921679218094 2213 3 -0.898151044999107 2213 4 -1.5505583614563854 2213 5 -2.5965994801796697 2213 6 -1.0106024223851477 2213 7 -0.7488202020175336 2213 8 -0.5601100920915094 2213 9 -1.0302587980744562 2213 10 -0.8055103860899706 2213 11 -0.9662473937272082 2213 12 -1.2401490608116532 2213 13 -0.995093941458876 2213 14 -8.063881834842036 2213 15 -3.850024063991505 2213 16 -2.3948051987094807 2213 17 -1.965618895804802 2213 18 -5.257713331625997 2213 19 -3.540111709745254 2213 20 -6.060174320567061 2213 21 -3.125369957006342 2213 22 -2.7325599547778268 2213 23 -4.944234022270479 2213 24 -2.9392672488762983 2213 25 -2.5416790402474376 2214 1 0.17978606275152131 2214 2 -0.06903367578192743 2214 3 -1.9086219323008855 2214 4 -2.249001995053149 2214 5 -0.6253282746874262 2214 6 -0.9626213754693502 2214 7 -1.0707494046391481 2214 8 -1.1702835404806713 2214 9 -5.7291970103282495 2214 10 -1.9179405657638213 2214 11 -1.4032335766521413 2214 12 -1.938162099703522 2214 13 -1.7926677391658323 2214 14 -3.607366712148483 2214 15 -2.874026692521433 2214 16 -2.1549034227224837 2214 17 -2.341116500729836 2214 18 -1.7793814105510677 2214 19 -4.783496287883494 2214 20 -2.7749217568857354 2214 21 -4.698377375686196 2214 22 -6.647158671640192 2214 23 -6.705053362716981 2214 24 -6.099654265321902 2214 25 -4.089568434475048 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20369.0 3 16.0 4 390.0 5 2518.0 6 3157.0 7 2461.0 8 1933.0 9 1635.0 10 1352.0 11 1170.0 12 1160.0 13 1140.0 14 1132.0 15 1119.0 16 1115.0 17 1203.0 18 1363.0 19 1604.0 20 2093.0 21 2745.0 22 3393.0 23 4190.0 24 4992.0 25 5634.0 26 6593.0 27 7334.0 28 8273.0 29 9809.0 30 11984.0 31 14982.0 32 19341.0 33 24627.0 34 31827.0 35 41604.0 36 56340.0 37 81670.0 38 130889.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.95221377023601 19.623366491125417 9.451336063975035 29.97308367466355 2 11.969450649700002 20.838352767803887 43.1562203950831 24.035976187413013 3 19.911224955261588 24.08564043182802 28.255767258890174 27.74736735402022 4 11.354115291718731 17.51540517411112 38.068373435846055 33.062106098324094 5 12.019328229038345 39.57589390135508 35.71434140560433 12.690436464002245 6 32.032969505359596 38.425164192127774 17.298230031361335 12.24363627115129 7 28.241709095241163 31.00186119362468 20.80611362069538 19.950316090438776 8 23.273392044156164 42.62745580575038 18.84508038070238 15.254071769391075 9 25.7334213569174 16.054407950248446 19.588112128504015 38.624058564330134 10 14.9415094118977 31.15671423615307 33.62146590940098 20.28031044254825 11 34.09634351438098 22.525689720019564 25.05968121946514 18.318285546134312 12 24.58441978571067 24.86543580869084 32.129173828482784 18.420970577115707 13 30.535684010936226 22.77119867798744 25.134023310604746 21.55909400047159 14 19.23022918389316 25.304031389924646 26.674610356178917 28.791129070003272 15 22.12131900854148 34.9612096433158 22.105533934984983 20.811937413157736 16 19.59863040127216 29.24447960899788 31.742808534852855 19.4140814548771 17 19.853565944045588 31.142668942884093 29.22932111035757 19.77444400271275 18 19.49187059206411 28.64751518600296 33.23303523230638 18.627578989626553 19 23.461216917503496 27.235169512168977 27.174953083802173 22.128660486525355 20 23.048771219387664 29.161007188917104 28.921311660700262 18.86890993099497 21 20.802795245899404 27.830079293471588 27.30957582182451 24.0575496388045 22 21.68420191091615 31.467952303096325 26.80797494723837 20.039870838749156 23 20.349717532840828 30.437078710803906 28.374552037680477 20.838651718674793 24 23.083818565894195 27.81123089274996 29.80594595015941 19.29900459119644 25 21.331093602932434 29.400164082337376 29.15072774998685 20.118014564743344 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 251.0 1 251.0 2 273.0 3 295.0 4 295.0 5 295.0 6 348.5 7 402.0 8 402.0 9 402.0 10 693.0 11 984.0 12 984.0 13 984.0 14 1500.0 15 2016.0 16 2016.0 17 2016.0 18 3574.0 19 5132.0 20 5132.0 21 5132.0 22 8699.0 23 12266.0 24 12266.0 25 12266.0 26 18961.5 27 25657.0 28 25657.0 29 25657.0 30 36205.5 31 46754.0 32 46754.0 33 46754.0 34 52206.0 35 57658.0 36 57658.0 37 57658.0 38 60528.5 39 63399.0 40 63399.0 41 63399.0 42 65867.0 43 68335.0 44 68335.0 45 68335.0 46 71299.0 47 74263.0 48 74263.0 49 74263.0 50 68036.0 51 61809.0 52 61809.0 53 61809.0 54 50607.5 55 39406.0 56 39406.0 57 39406.0 58 32430.5 59 25455.0 60 25455.0 61 25455.0 62 20370.0 63 15285.0 64 15285.0 65 15285.0 66 11681.0 67 8077.0 68 8077.0 69 8077.0 70 5837.0 71 3597.0 72 3597.0 73 3597.0 74 2513.5 75 1430.0 76 1430.0 77 1430.0 78 955.0 79 480.0 80 480.0 81 480.0 82 316.0 83 152.0 84 152.0 85 152.0 86 98.0 87 44.0 88 44.0 89 44.0 90 27.0 91 10.0 92 10.0 93 10.0 94 5.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08905656553452453 2 0.029425692331976377 3 0.034102623563548776 4 0.03507698423679303 5 0.02435901683110627 6 0.3932519677213796 7 0.21825679080671218 8 0.22527218765407078 9 0.2285850139431012 10 0.016174387175854565 11 0.002728209885083902 12 0.0037025705583281533 13 0.0 14 0.07424628330121191 15 0.002728209885083902 16 0.002728209885083902 17 0.004871803366221254 18 0.003897442692977003 19 0.001558977077190801 20 0.0011692328078931008 21 0.0 22 0.0 23 0.0 24 1.9487213464885013E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 513157.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.3821832355867 #Duplication Level Percentage of deduplicated Percentage of total 1 93.7856751808765 72.57340301719799 2 4.364943371492806 6.755376955716316 3 0.7487679352095512 1.738238926899522 4 0.32798054283475647 1.0151940185338524 5 0.17511695736711838 0.6775466241320388 6 0.10456172050394193 0.48547285292785414 7 0.07551912138373185 0.40906841420945156 8 0.05270725519318574 0.3262881983363141 9 0.041962441917322624 0.29224308325130305 >10 0.253305239930379 3.7368337472231534 >50 0.036251146519049385 1.9467645389591934 >100 0.025603952396497286 4.067921312215648 >500 0.004816585104291369 2.3086929605760202 >1k 0.0027885492709055293 3.666955349821357 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 2702 0.5265445078211931 No Hit GTCCTACAGTGGACATTTCTAAATT 2640 0.5144624354729644 No Hit GTCCTACAGTGTGCATTTCTCATTT 1804 0.3515493309065257 No Hit GTACATGGGAAGCAGTGGTATCAAC 1763 0.3435595733859228 No Hit CTGTAGGACCTGGAATATGGCGAGA 1676 0.32660569767147285 No Hit CCCATGTACTCTGCGTTGATACCAC 1632 0.31803132374692344 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1622 0.31608260240043495 No Hit CTTTAGGACGTGAAATATGGCGAGG 1593 0.3104313104956183 No Hit GTATCAACGCAGAGTACTTTTTTTT 1218 0.2373542600022995 No Hit GATATACACTGTTCTACAAATCCCG 1025 0.1997439380150714 No Hit CTGAAGGACCTGGAATATGGCGAGA 1018 0.19837983307252946 No Hit GGTATCAACGCAGAGTACTTTTTTT 838 0.16330284883573642 No Hit GAGTACATGGGAAGCAGTGGTATCA 771 0.15024641581426346 No Hit GTGTATATCAATGAGTTACAATGAA 751 0.14634897312128647 No Hit GTGTATATCAATGAGTTACAATGAG 685 0.13348741223446237 No Hit GTTCTACAGTGTGGTTTTTATCATT 672 0.13095407448402732 No Hit CTGTAGGACATGGAATATGGCAAGA 660 0.1286156088682411 No Hit ACCTGGAATATGGCGAGAAAACTGA 641 0.12491303830991295 No Hit GATATACACTGTTCTACAATGCCGG 635 0.12374380550201984 No Hit CATGTACTCTGCGTTGATACCACTG 635 0.12374380550201984 No Hit GTCCTTCAGTGTGCATTTCTCATTT 597 0.11633866438536355 No Hit CTGTAGAACATATTAGATGAGTGAG 589 0.11477968730817273 No Hit TATCAACGCAGAGTACTTTTTTTTT 578 0.11263609382703539 No Hit GAATATGGCAAGAAAACTGAAAATC 553 0.10776429046081414 No Hit GACCTGGAATATGGCGAGAAAACTG 543 0.10581556911432564 No Hit ACAGTGGACATTTCTAAATTTTCCA 534 0.104061719902486 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 532 0.10367197563318829 No Hit ACGCAGAGTACATGGGAAGCAGTGG 529 0.10308735922924173 No Hit TTGTAGAACAGTGTATATCAATGAG 523 0.10191812642134862 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.9487213464885016E-4 0.0 10 0.0 0.0 0.0 1.9487213464885016E-4 0.0 11 0.0 0.0 0.0 1.9487213464885016E-4 0.0 12 0.0 0.0 0.0 1.9487213464885016E-4 0.0 13 0.0 0.0 0.0 1.9487213464885016E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAATAC 35 0.0021103078 16.351048 3 TAGGACC 525 0.0 14.714499 4 CTAGGAC 85 1.61981E-8 14.587698 3 GTATAAC 40 0.0051545408 14.298751 1 ACTGGGC 40 0.005266228 14.252639 8 CCGGTTT 60 2.6118885E-5 14.220675 12 CCAACGA 115 2.5465852E-11 14.005019 19 GGACCTA 55 1.892342E-4 13.872255 6 TAGTACA 55 1.892342E-4 13.872255 4 CGGTTTC 70 7.395549E-6 13.543501 13 GTAGGAC 1385 0.0 13.497976 3 TAATACT 50 0.0014537146 13.352045 4 CTGTAGG 1430 0.0 13.332168 1 TGTAGGA 1455 0.0 13.310079 2 AAGTGTA 65 5.419169E-5 13.158854 7 TATGTCG 65 5.5803448E-5 13.117824 16 AGGACCT 835 0.0 13.020814 5 GTCCTAT 110 2.59206E-9 12.998864 1 TAGGACA 390 0.0 12.960777 4 GTAGGAA 75 1.4145271E-5 12.717481 3 >>END_MODULE