FastQCFastQC Report
Thu 26 May 2016
SRR1926514_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926514_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373561
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC8700.23289369072253258No Hit
CCCATGTACTCTGCGTTGATACCAC8340.22325671041677264No Hit
GTCCTACAGTGGACATTTCTAAATT5150.137862357151844No Hit
GAGTACATGGGAAGCAGTGGTATCA4880.13063462192252404No Hit
CTGTAGGACGTGGAATATGGCAAGA4510.12072994771938184No Hit
GTATCAACGCAGAGTACTTTTTTTT4040.10814833454241743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAC350.005906020819.0604321
CCTATTC358.530901E-418.712283
GTCCTAG501.12682814E-418.6792241
GTGTAGA652.1644446E-618.4739571
GCAATAT300.00105586918.1291712
GATATAC2300.017.4030041
CTGTAGG3100.016.7854791
GTCCTAA1206.002665E-1116.6778771
AACAGGG555.920896E-616.6276767
GAACAGG504.0898038E-516.596826
TATAGGA500.00207964616.3803312
TGTAGAA1850.016.2327612
GTAGGAC3050.016.1048323
TAGGACT400.00238883716.0462134
GTACTGG500.002422241316.0107631
TGTAGGA3300.015.71852
TGAACAG400.002930376415.5623645
GTCCTAC3700.015.5059191
TATACAC2550.014.9820553
CTAGAGA506.0872955E-414.9764654
Produced by FastQC (version 0.11.5)