FastQCFastQC Report
Thu 26 May 2016
SRR1926514_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926514_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373561
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC24350.651834640125709No Hit
CCCATGTACTCTGCGTTGATACCAC23330.6245298625927225No Hit
GTATCAACGCAGAGTACTTTTTTTT18030.48265209698014516No Hit
GTCCTACAGTGGACATTTCTAAATT12200.32658655480631005No Hit
GAGTACATGGGAAGCAGTGGTATCA12090.32364192193510566No Hit
CTGTAGGACGTGGAATATGGCAAGA12010.32150037075604787No Hit
GGTATCAACGCAGAGTACTTTTTTT11800.3158787989110212No Hit
TATCAACGCAGAGTACTTTTTTTTT9460.2532384269235814No Hit
CATGTACTCTGCGTTGATACCACTG8960.23985373205447036No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7820.20933662775289713No Hit
GATATACACTGTTCTACAAATCCCG7810.2090689338555149No Hit
GTATCAACGCAGAGTACATGGGAAG7730.20692738267645713No Hit
ACGCAGAGTACATGGGAAGCAGTGG7720.2066596887790749No Hit
GTCCTACAGTGTGCATTTCTCATTT7660.20505352539478158No Hit
CTTTAGGACGTGAAATATGGCGAGG7350.19675501457593272No Hit
GTCCTAAAGTGTGTATTTCTCATTT7130.19086574883352384No Hit
GCGTTGATACCACTGCTTCCCATGT6960.18631495257802608No Hit
GTGTATATCAATGAGTTACAATGAA6680.17881952345132388No Hit
CTGTAGGACCTGGAATATGGCGAGA6170.1651671346848306No Hit
GATATACACTGTTCTACAATGCCGG5120.13705927545969734No Hit
ACTCTGCGTTGATACCACTGCTTCC5060.13545311207540403No Hit
GGTATCAACGCAGAGTACATGGGAA4980.13331156089634624No Hit
TATCAACGCAGAGTACATGGGAAGC4870.13036692802514183No Hit
GCTTCCCATGTACTCTGCGTTGATA4870.13036692802514183No Hit
GTGTATATCAATGAGTTACAATGAG4850.12983154023037735No Hit
CTGAAGGACCTGGAATATGGCGAGA4600.12313919279582183No Hit
TTGTAGAACAGTGTATATCAATGAG4560.12206841720629295No Hit
GTTCTACAGTGTGGTTTTTATCATT4100.10975449792671077No Hit
CTGTAGAACATATTAGATGAGTGAG3960.10600678336335967No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG250.005890434619.073694
GTCTTAG359.673422E-519.060851
TCCAACG1050.016.24383718
CCAACGT350.002203623316.24383719
TAGGACC2650.015.1154
GTATTAC456.583634E-414.8251071
CAGATTA456.7467213E-414.777369
GTAGGAC6750.014.4112323
TGTAGGA6850.014.3420062
TAAGCCC400.005135488714.3052684
ACAGTCC602.5586198E-514.2495988
GATATAC3350.014.2245151
CTGTAGG6650.014.0448371
AGGACCT4200.013.6240635
GGACCTG4100.013.4893286
CCAACGA852.7658461E-713.37727819
TATACAC4000.013.35158353
AGACTGT655.2436233E-513.2030846
GCCGGTT655.528816E-513.12880911
GTGTAAC957.027484E-813.0416351
Produced by FastQC (version 0.11.5)