FastQCFastQC Report
Thu 26 May 2016
SRR1926513_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926513_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629339
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC20990.3335245392387886No Hit
CCCATGTACTCTGCGTTGATACCAC19930.31668147055879264No Hit
GTCCTACAGTGGACATTTCTAAATT11180.17764670551165587No Hit
GAGTACATGGGAAGCAGTGGTATCA9990.15873797746524526No Hit
CTGTAGGACGTGGAATATGGCAAGA7780.12362176823619703No Hit
GCGTTGATACCACTGCTTCCCATGT7590.12060272762374491No Hit
CATGTACTCTGCGTTGATACCACTG7170.11392905890148235No Hit
GTCCTACAGTGTGCATTTCTCATTT6880.1093210495456344No Hit
GTCCTAAAGTGTGTATTTCTCATTT6790.10789097767657813No Hit
GTATCAACGCAGAGTACATGGGAAG6540.1039185558180885No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAAG405.260537E-420.1087271
GACCTAC450.001161453217.8744241
TAACGCA300.00857281517.8734134
TGTAGGA5650.017.2655772
CCTGTAC1200.016.450273
GTCCAGT500.002350800716.086981
CTGTAGG6000.015.863551
GTATAGA604.3842473E-415.64012051
GTCCAGA604.3842473E-415.64012051
GATATAC2500.015.5507481
TAGGACC2600.015.2611464
GTCCTAA2650.015.1763961
GTAGGAC6200.014.8583093
TGTAGAA2700.014.7572492
TCCAACG1400.014.56544518
GTCTTAC751.4605824E-414.2995391
GTCCTAC7600.014.1113871
TGGATTG450.00620299313.90451055
CGTGTAA400.00644921813.823539
GCTTGTG450.00717846913.6012327
Produced by FastQC (version 0.11.5)