##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926513_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 629339 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.85370682573303 27.0 2.0 31.0 2.0 31.0 2 16.03432172485735 16.0 2.0 30.0 2.0 31.0 3 19.613121068295467 22.0 15.0 30.0 2.0 31.0 4 21.454279807861898 27.0 2.0 35.0 2.0 35.0 5 22.4748394744327 29.0 2.0 35.0 2.0 37.0 6 23.079356276982676 30.0 2.0 35.0 2.0 37.0 7 22.841625896376993 30.0 2.0 35.0 2.0 37.0 8 23.051067866444 30.0 7.0 35.0 2.0 37.0 9 25.743340234754243 31.0 17.0 37.0 2.0 39.0 10 26.479399814726243 31.0 20.0 37.0 2.0 39.0 11 23.557222736871545 30.0 7.0 37.0 2.0 39.0 12 24.732673487579827 31.0 10.0 37.0 2.0 39.0 13 21.404454514975235 27.0 2.0 35.0 2.0 39.0 14 24.72609197904468 30.0 12.0 38.0 2.0 40.0 15 25.838033238048176 31.0 16.0 38.0 2.0 40.0 16 26.186595777474462 31.0 12.0 38.0 2.0 40.0 17 25.521564689300998 31.0 10.0 38.0 2.0 40.0 18 25.579439697841703 31.0 10.0 38.0 2.0 40.0 19 25.969100913815925 31.0 10.0 38.0 2.0 40.0 20 26.22669658165154 31.0 10.0 39.0 2.0 40.0 21 24.087297942762167 30.0 2.0 37.0 2.0 40.0 22 25.02805642110214 31.0 2.0 38.0 2.0 40.0 23 24.998827341067376 31.0 2.0 38.0 2.0 40.0 24 24.09791702087428 31.0 2.0 38.0 2.0 40.0 25 23.88939824164719 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.047651339790484 1101 2 10.503261000820633 1101 3 7.247402596108632 1101 4 8.632556794716994 1101 5 8.65172203972402 1101 6 7.9698560753551675 1101 7 7.464707316542601 1101 8 6.120237224266418 1101 9 6.142116817434339 1101 10 5.874323255647127 1101 11 8.764439080076382 1101 12 6.5611142827828 1101 13 10.848005548615124 1101 14 8.291267621349654 1101 15 7.550388247779264 1101 16 6.855305836711086 1101 17 3.186174289940439 1101 18 5.3524651724640435 1101 19 6.058691585299346 1101 20 3.9927542827700684 1101 21 1.1928305630079272 1101 22 4.8658601613607395 1101 23 6.33345097702313 1101 24 7.415713401240637 1101 25 7.91944769886776 1102 1 5.533301465762662 1102 2 10.188275788858753 1102 3 6.813192412966476 1102 4 7.571177308099255 1102 5 7.814009673585758 1102 6 6.500086789614798 1102 7 6.514603252570641 1102 8 6.56073558375919 1102 9 5.6055170751165555 1102 10 5.2097117872236325 1102 11 7.760286953676474 1102 12 5.234689858264389 1102 13 9.736098961026642 1102 14 7.201253589666219 1102 15 5.176287397010654 1102 16 5.631127524790411 1102 17 6.278488683109252 1102 18 6.301565110390797 1102 19 5.051287133760816 1102 20 5.690572329914438 1102 21 4.452820965127806 1102 22 5.522722906458778 1102 23 6.0579448714151525 1102 24 7.594421756090956 1102 25 7.084304330533463 1103 1 5.220590308999181 1103 2 9.249499458680326 1103 3 6.365823072491807 1103 4 7.9657675023896815 1103 5 7.706917601375821 1103 6 7.101707565169352 1103 7 6.829641282624344 1103 8 6.444978878276363 1103 9 5.54284871744888 1103 10 4.93966521481979 1103 11 5.657590086570288 1103 12 4.779969554722719 1103 13 8.735103226961009 1103 14 6.882066667672376 1103 15 5.708587612578345 1103 16 5.5531912447830045 1103 17 3.3488608780215756 1103 18 2.1834011741317525 1103 19 4.1724382722167626 1103 20 4.785661439378803 1103 21 6.928869193594622 1103 22 2.730352675064154 1103 23 4.9327217047013185 1103 24 6.179420760356891 1103 25 2.4537566069212886 1104 1 5.665084883080759 1104 2 10.371646467530002 1104 3 7.010683347005482 1104 4 7.339583828155899 1104 5 7.749182560532464 1104 6 7.285797212850696 1104 7 7.243362880401538 1104 8 7.334681380634347 1104 9 6.170685534697409 1104 10 5.0568476870772905 1104 11 8.125225257826369 1104 12 5.19572565909376 1104 13 9.352900307486607 1104 14 7.344526190354994 1104 15 6.409942474793727 1104 16 6.069222135232913 1104 17 6.383244890715535 1104 18 4.463878763913932 1104 19 4.8706928528360045 1104 20 4.149485698869476 1104 21 3.7630424348104903 1104 22 5.562952791516249 1104 23 5.829004431314708 1104 24 6.690945091012736 1104 25 5.941449118915514 1105 1 3.870699468132731 1105 2 9.652494512407085 1105 3 6.848338849397827 1105 4 6.034761191627272 1105 5 2.3094828410142334 1105 6 5.010936628788659 1105 7 6.434422111551513 1105 8 3.230771134800328 1105 9 4.387893916152617 1105 10 4.029781196399185 1105 11 7.237371768303188 1105 12 4.40935212337358 1105 13 4.859689616181544 1105 14 4.084729127164103 1105 15 4.784101854434049 1105 16 5.166136835777376 1105 17 5.734120983769486 1105 18 3.1987277987266687 1105 19 2.9006252072329666 1105 20 2.2704030392423533 1105 21 2.945758158288463 1105 22 4.504238057973929 1105 23 2.058931836033402 1105 24 5.246031710382475 1105 25 5.943879410946533 1106 1 6.767840681349895 1106 2 11.338314009365824 1106 3 7.990030448937528 1106 4 9.237061043673958 1106 5 9.086740772955707 1106 6 8.142662750767947 1106 7 7.881400576124914 1106 8 7.743906101099849 1106 9 6.826328898891397 1106 10 6.1495658421332 1106 11 8.516073067004488 1106 12 5.778023831412376 1106 13 4.823120489106088 1106 14 6.234719263609936 1106 15 6.599679694315686 1106 16 6.6070048285441025 1106 17 6.8362942535376945 1106 18 6.8104720038885915 1106 19 6.707174607200091 1106 20 5.907154642069905 1106 21 6.205199223552313 1106 22 6.610124955886143 1106 23 7.0723676407350275 1106 24 7.872508777619036 1106 25 6.836253239649476 1107 1 4.076622575862007 1107 2 -13.594778214865642 1107 3 -5.390151338828042 1107 4 1.789390747093833 1107 5 4.6734556083350185 1107 6 4.850307874283253 1107 7 4.622019228091361 1107 8 5.444787954984548 1107 9 3.6902167034974305 1107 10 3.013021084264622 1107 11 5.8054374471177645 1107 12 3.2051350629010145 1107 13 4.998075679457475 1107 14 4.062426363848125 1107 15 3.853120677021156 1107 16 3.4969738401826653 1107 17 4.283772565667764 1107 18 -0.7925176958668452 1107 19 1.9955631327435803 1107 20 2.418076247091772 1107 21 -1.3887552578108568 1107 22 2.0459240812018002 1107 23 -1.0795263137463351 1107 24 2.319295627082518 1107 25 3.2100748454679575 1108 1 5.329941758172186 1108 2 10.27590380462063 1108 3 0.5450640285587909 1108 4 5.711254468058545 1108 5 7.316092068810352 1108 6 7.0915365160581665 1108 7 7.117827377676001 1108 8 6.8130402385929045 1108 9 5.3712661683468355 1108 10 4.477518534349102 1108 11 7.034989934017013 1108 12 2.7560861599304687 1108 13 3.6971684792139925 1108 14 4.976489333299863 1108 15 4.895977206716843 1108 16 4.892354727717148 1108 17 0.36729273672866114 1108 18 3.492215197067921 1108 19 4.670076853903392 1108 20 4.9040273246115476 1108 21 4.939899393492585 1108 22 1.219652342537902 1108 23 4.390189586254834 1108 24 4.913233578487798 1108 25 5.69867689866696 1109 1 2.7635820333975687 1109 2 -13.594778214865642 1109 3 -5.756629672385099 1109 4 1.2995131432767302 1109 5 1.1375832355412214 1109 6 1.883864522686082 1109 7 2.9100932271395266 1109 8 3.0882713446551087 1109 9 2.3515050972153198 1109 10 1.7947564335350883 1109 11 0.2964450495925348 1109 12 0.5026476828464261 1109 13 2.1455579952632178 1109 14 2.555625626786366 1109 15 2.2437056486084828 1109 16 2.207041729590859 1109 17 2.537589463581458 1109 18 2.8421543075825397 1109 19 1.5002340379221302 1109 20 0.31207684097155663 1109 21 -0.3834217292293758 1109 22 1.8011162582593307 1109 23 2.2793658157194585 1109 24 3.40346140903101 1109 25 3.7709934880052067 1110 1 2.7203189184498555 1110 2 8.590338064204126 1110 3 5.732250054471823 1110 4 7.308312988667442 1110 5 7.741702714164337 1110 6 3.935397516737922 1110 7 6.207762492101196 1110 8 6.710432868415548 1110 9 5.217703641311179 1110 10 4.741557094919269 1110 11 2.4534817048782394 1110 12 5.22861216565688 1110 13 9.548547967132919 1110 14 7.1877946662761545 1110 15 6.522748788894937 1110 16 6.024276370661099 1110 17 6.6604634784840755 1110 18 6.618304965745697 1110 19 5.306502585668486 1110 20 5.516111284485483 1110 21 3.3252084754131985 1110 22 5.735591123513039 1110 23 6.0247457895217735 1110 24 7.192374205097064 1110 25 7.508044315809112 1111 1 4.605270565824849 1111 2 9.046714050830445 1111 3 7.039465493454408 1111 4 7.598949296516015 1111 5 7.825611553066512 1111 6 7.0951512040223434 1111 7 6.95650913281051 1111 8 7.016708764050058 1111 9 5.624183104671104 1111 10 4.762343856668853 1111 11 6.7244443135922936 1111 12 5.9896143452281585 1111 13 7.804858778229686 1111 14 5.495422319322262 1111 15 5.239208149412956 1111 16 5.240659128771007 1111 17 6.523777696483524 1111 18 6.109921445843881 1111 19 5.164039881730446 1111 20 4.498906637554871 1111 21 7.388593793990342 1111 22 6.597184538245248 1111 23 6.614609725023392 1111 24 6.515233464042829 1111 25 7.063387227970274 1112 1 -2.1878017603684974 1112 2 7.254771334683909 1112 3 5.521778652837632 1112 4 -0.8241822973162165 1112 5 3.5551994667308584 1112 6 2.4906499785020095 1112 7 5.545470432599835 1112 8 5.525857129886322 1112 9 4.180850509109209 1112 10 3.1911653077832973 1112 11 -0.533733882802462 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0.7559761054385312 2204 6 3.5047848029816606 2204 7 5.0410280591919445 2204 8 4.662390214695268 2204 9 3.4153022021815893 2204 10 2.9806962180038603 2204 11 4.863563414494834 2204 12 4.9201824207556015 2204 13 2.5167301990841935 2204 14 4.002249249166983 2204 15 4.301687214088375 2204 16 4.012641961592848 2204 17 -1.0781030270752154 2204 18 2.7409916299560173 2204 19 3.3538438804516417 2204 20 3.7115840949061685 2204 21 5.178061809902459 2204 22 4.767985410341971 2204 23 3.8237314632468227 2204 24 5.411011800190153 2204 25 5.4715172996189025 2205 1 6.018051210626286 2205 2 10.25334621331368 2205 3 7.111227479523421 2205 4 7.812710954581608 2205 5 8.08311338247643 2205 6 7.619228236540469 2205 7 7.7129357936608045 2205 8 7.363633149272587 2205 9 6.3739622851121105 2205 10 5.550919847318259 2205 11 7.865961970415803 2205 12 6.831087963014269 2205 13 10.113291046772911 2205 14 7.4218404970385095 2205 15 6.357873791554567 2205 16 6.1590920050363 2205 17 6.915117407291067 2205 18 6.572755911510498 2205 19 5.280725015932958 2205 20 5.514573379423673 2205 21 -0.5320693390083839 2205 22 3.882131262664938 2205 23 5.282930006207234 2205 24 7.175555123840017 2205 25 7.4849348879489455 2206 1 4.141886313925198 2206 2 8.535774822305708 2206 3 5.924363740191531 2206 4 1.8524146985917476 2206 5 5.7531126110628605 2206 6 6.069272572083804 2206 7 6.162145675385684 2206 8 6.158027378103714 2206 9 4.998913449656282 2206 10 4.189111123045521 2206 11 3.8612356107164416 2206 12 5.1173081111754435 2206 13 8.194936463622408 2206 14 0.8262926316559849 2206 15 3.1163906535768824 2206 16 3.684087878388439 2206 17 4.784439948576214 2206 18 -0.40707950367174917 2206 19 1.3471545074884688 2206 20 3.102337433044383 2206 21 3.8133655101387873 2206 22 -1.8379600793375346 2206 23 -2.779753567411749 2206 24 1.2455256525237424 2206 25 2.80243129424003 2207 1 1.5325774696492864 2207 2 -13.594778214865642 2207 3 -6.076580688895204 2207 4 -4.296195923546712 2207 5 1.1943155986180756 2207 6 1.974410383949163 2207 7 2.868385377201527 2207 8 3.2554741426358653 2207 9 0.7996809243251661 2207 10 0.40958994187058195 2207 11 -1.309294336676171 2207 12 -2.913287526133537 2207 13 0.17160721665335998 2207 14 -5.703604346591057 2207 15 -1.7465763829911758 2207 16 -0.3232978259464403 2207 17 0.6233584932479594 2207 18 0.8108113777981991 2207 19 0.33838913005713067 2207 20 0.22650549100745465 2207 21 -3.752848295498712 2207 22 -3.8163255952403254 2207 23 -0.9285658470064497 2207 24 0.7000699612881967 2207 25 1.2544641274348614 2208 1 4.74734981170749 2208 2 6.136759578357644 2208 3 6.0747009061074095 2208 4 8.207498732105908 2208 5 3.8077468012069495 2208 6 6.210638863999151 2208 7 6.676424323067192 2208 8 6.760729273967851 2208 9 5.081401537983435 2208 10 4.771393233363089 2208 11 6.506764391318747 2208 12 6.29456735123868 2208 13 8.987590255941434 2208 14 3.149070806336365 2208 15 4.836010866360439 2208 16 5.329559946116213 2208 17 6.182019254048296 2208 18 1.2686733006617459 2208 19 3.313197673862774 2208 20 4.567190022892934 2208 21 7.144285920708494 2208 22 6.082381174631379 2208 23 2.0952567708726697 2208 24 3.527101136178228 2208 25 4.706439240343894 2209 1 3.2380317654650277 2209 2 4.850615897195137 2209 3 5.252198816591129 2209 4 0.40798833485441577 2209 5 5.181844060042117 2209 6 5.630058617910649 2209 7 6.319855073651141 2209 8 4.409343180039038 2209 9 4.490501185426552 2209 10 3.343227626512281 2209 11 -0.6963811454497275 2209 12 2.9870202577083838 2209 13 1.9877263942183205 2209 14 3.6607362065045166 2209 15 4.188285571951102 2209 16 3.8634777664516413 2209 17 0.8520109949928312 2209 18 3.367659854325389 2209 19 3.8494933761011367 2209 20 4.0309883798276935 2209 21 6.04622263208627 2209 22 4.8793230963923016 2209 23 0.09352822729645993 2209 24 -3.129962418944988 2209 25 -0.25656002615957263 2210 1 -1.963422725463147 2210 2 -13.594778214865642 2210 3 -7.319276167164556 2210 4 -2.2741604130838056 2210 5 -0.14168020646460633 2210 6 -1.1410839821793175 2210 7 -0.9819268026921399 2210 8 -20.514683410654218 2210 9 -12.65526029805423 2210 10 -6.97257434279846 2210 11 -10.007784654221656 2210 12 -4.330206213026862 2210 13 -5.176766033432001 2210 14 -3.744821848176347 2210 15 -5.454639404307208 2210 16 -4.7794097229270776 2210 17 -4.483221673573169 2210 18 -4.4571389660874665 2210 19 -9.519227571567182 2210 20 -6.678938145888306 2210 21 -5.283237057022543 2210 22 -9.73807331486376 2210 23 -7.775805006071863 2210 24 -10.45892228299257 2210 25 -8.1431872066464 2211 1 -0.27269299306460937 2211 2 4.294977882695335 2211 3 0.018693616581863637 2211 4 1.0730095286560974 2211 5 0.46841853946212453 2211 6 -0.5828327725417175 2211 7 -20.33921425208485 2211 8 -13.299073654556658 2211 9 -9.465631582738737 2211 10 -7.581460488744938 2211 11 -10.13340428491189 2211 12 -5.29004920700336 2211 13 -7.270487767410474 2211 14 -5.430991493678469 2211 15 -5.712350473725596 2211 16 -12.189472920320183 2211 17 -8.251777020665102 2211 18 -6.287968431872002 2211 19 -9.09063865086016 2211 20 -7.099929554992681 2211 21 -4.834020112221513 2211 22 -6.344431565085937 2211 23 -8.507165724942208 2211 24 -21.606695562344797 2211 25 -21.395211498144377 2212 1 -1.0736354982980423 2212 2 -13.594778214865642 2212 3 -7.638964725271016 2212 4 -19.0079660811 2212 5 -19.915971704440313 2212 6 -20.54088155302952 2212 7 -9.843206268052915 2212 8 -9.150411953568389 2212 9 -5.09731747923842 2212 10 -5.362969665513354 2212 11 -5.135195823785363 2212 12 -2.928570213770314 2212 13 -5.796389135406191 2212 14 -3.658306619524339 2212 15 -3.7399917764859296 2212 16 -3.973384122306456 2212 17 -3.9299436143070885 2212 18 -3.0723518601374202 2212 19 -3.5551759177897146 2212 20 -3.8460694702840463 2212 21 -1.6188290309693834 2212 22 -2.907416814100106 2212 23 -3.032265768757018 2212 24 -1.9619849835024823 2212 25 -2.4181655899607435 2213 1 -18.422936895503632 2213 2 -13.594778214865642 2213 3 -8.649742768383188 2213 4 -5.97790596085952 2213 5 -19.915971704440313 2213 6 -10.847494779482428 2213 7 -20.33921425208485 2213 8 -10.708070184201311 2213 9 -8.776890197529294 2213 10 -7.6838369489824885 2213 11 -6.5563575084321 2213 12 -5.496224484387394 2213 13 -18.881219474727548 2213 14 -12.41338163982442 2213 15 -7.310582058847057 2213 16 -6.8320595570081935 2213 17 -5.280971018416704 2213 18 -5.299282767861051 2213 19 -6.263444750865048 2213 20 -6.12559258837652 2213 21 -4.222589446011046 2213 22 -5.113883639967408 2213 23 -4.988783594828323 2213 24 -3.994471113447002 2213 25 -4.725872820789668 2214 1 -18.422936895503632 2214 2 -13.594778214865642 2214 3 -12.212515779843898 2214 4 -19.0079660811 2214 5 -19.915971704440313 2214 6 -20.54088155302952 2214 7 -16.258569090794527 2214 8 -14.779660369179563 2214 9 -13.91604408133377 2214 10 -13.911421149641871 2214 11 -14.253512186451735 2214 12 -12.2538988192322 2214 13 -18.881219474727548 2214 14 -16.538978455575737 2214 15 -23.37771632738413 2214 16 -18.01842537620419 2214 17 -23.098606288957786 2214 18 -17.61322863193944 2214 19 -14.940028332775412 2214 20 -13.575097589161501 2214 21 -11.208716077153515 2214 22 -12.177353898456738 2214 23 -12.111482910510379 2214 24 -11.036419064648944 2214 25 -10.789220714734238 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 120462.0 3 6.0 4 447.0 5 2047.0 6 2993.0 7 2797.0 8 2345.0 9 2281.0 10 2116.0 11 1942.0 12 1895.0 13 2079.0 14 3061.0 15 6237.0 16 9365.0 17 10131.0 18 10437.0 19 12396.0 20 14214.0 21 16579.0 22 17206.0 23 18644.0 24 19619.0 25 19678.0 26 16325.0 27 13295.0 28 14344.0 29 16444.0 30 16034.0 31 18563.0 32 23499.0 33 29587.0 34 35939.0 35 40540.0 36 39260.0 37 39999.0 38 26533.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.667105638796535 18.628933481908515 12.683838765229725 25.02012211406522 2 13.272268624358984 20.040441537080213 42.74403877156316 23.943251066997643 3 20.40042933326572 24.3144612628145 28.037152540080967 27.247956863838812 4 12.030736873178112 16.90306630751633 37.43109110364931 33.63510571565625 5 12.16491691119008 38.102891520075495 36.68680605729958 13.045385511434848 6 31.538164952852966 37.23062180180652 17.97950910186679 13.25170414347372 7 28.061602797251943 30.972537030203995 21.367701579603956 19.598158592940102 8 24.36415170924001 39.076205480300224 19.975990148393265 16.5836526620665 9 26.49456673035043 16.049103840592274 19.614175019526684 37.84215440953061 10 15.044781510734984 30.20196025943282 33.906715130248564 20.84654309958363 11 33.04777990238097 22.465058167352232 24.30500961143431 20.182152318832493 12 24.647485367476452 24.882551412253832 31.73965760063765 18.730305619632066 13 29.087941617293893 21.42711588746246 24.578593642577324 24.906348852666323 14 19.96850978618952 24.087591240875913 26.833376396873586 29.110522576060976 15 23.57140869212169 32.019763305278566 22.811785295066215 21.597042707533532 16 20.279087605776464 28.293268167000203 30.679313131114466 20.748331096108867 17 21.396097838787913 28.99601666650301 29.4069513588175 20.20093413589157 18 21.05932312775899 27.221874435294417 32.39086661331543 19.327935823631154 19 23.207365513103976 27.105255943194333 27.092676900846506 22.594701642855185 20 23.149717964243603 28.384429076771088 28.376143280537942 20.08970967844737 21 21.092337105358343 26.83171567965623 26.987230453559757 25.088716761425665 22 21.817441807880407 29.6006176149468 27.066845835298377 21.51509474187442 23 20.78071237339552 28.93891647087083 28.476483024222475 21.803888131511172 24 22.510059525788968 28.506744564180565 28.511645246600587 20.471550663429884 25 22.134180485383766 27.67937818954656 28.10237092278911 22.084070402280563 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 809.0 1 809.0 2 1620.5 3 2432.0 4 2432.0 5 2432.0 6 3317.0 7 4202.0 8 4202.0 9 4202.0 10 4869.5 11 5537.0 12 5537.0 13 5537.0 14 6563.0 15 7589.0 16 7589.0 17 7589.0 18 10534.0 19 13479.0 20 13479.0 21 13479.0 22 19040.5 23 24602.0 24 24602.0 25 24602.0 26 32733.0 27 40864.0 28 40864.0 29 40864.0 30 49739.0 31 58614.0 32 58614.0 33 58614.0 34 63821.5 35 69029.0 36 69029.0 37 69029.0 38 71077.0 39 73125.0 40 73125.0 41 73125.0 42 75097.0 43 77069.0 44 77069.0 45 77069.0 46 79977.0 47 82885.0 48 82885.0 49 82885.0 50 74042.0 51 65199.0 52 65199.0 53 65199.0 54 54147.0 55 43095.0 56 43095.0 57 43095.0 58 35567.0 59 28039.0 60 28039.0 61 28039.0 62 22566.0 63 17093.0 64 17093.0 65 17093.0 66 13079.0 67 9065.0 68 9065.0 69 9065.0 70 6568.0 71 4071.0 72 4071.0 73 4071.0 74 2861.5 75 1652.0 76 1652.0 77 1652.0 78 1132.5 79 613.0 80 613.0 81 613.0 82 409.5 83 206.0 84 206.0 85 206.0 86 129.0 87 52.0 88 52.0 89 52.0 90 34.0 91 16.0 92 16.0 93 16.0 94 8.5 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.729325530437492 2 36.820854896963326 3 5.9942256875865 4 13.874716170458212 5 13.790183033309553 6 15.10362459660056 7 9.567021907112066 8 7.61290814648385 9 0.11313457452978443 10 1.4645524907879537 11 6.015200074999324 12 5.905402334830671 13 16.5653169436504 14 1.6046995339554675 15 2.941181144025716 16 3.0520911623147464 17 2.906700522294026 18 1.5169884593200167 19 1.4713850563845559 20 0.2794996019633298 21 4.6709325180864365 22 1.618046871399993 23 0.11249898703242608 24 9.214429742952527 25 0.11488244014751987 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 629339.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.06402034041508 #Duplication Level Percentage of deduplicated Percentage of total 1 96.37637882905354 83.90915006708266 2 2.390087018639712 4.161811696124198 3 0.5198824163814039 1.3578915982336406 4 0.2167876894138884 0.7549763120272946 5 0.11620117698447302 0.5058470818278167 6 0.07674650380731421 0.40091155011214485 7 0.05141699644176033 0.31335992968339293 8 0.0365631092769551 0.2546665031838111 9 0.02440410416069419 0.19122474789326588 >10 0.15920749253420324 2.6169211655771174 >50 0.01509777987845418 0.930410455335891 >100 0.014904157572290773 2.6427548310146207 >500 0.0017420443914841924 1.082076824992405 >1k 5.806814638280642E-4 0.8779972369117631 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2099 0.3335245392387886 No Hit CCCATGTACTCTGCGTTGATACCAC 1993 0.31668147055879264 No Hit GTCCTACAGTGGACATTTCTAAATT 1118 0.17764670551165587 No Hit GAGTACATGGGAAGCAGTGGTATCA 999 0.15873797746524526 No Hit CTGTAGGACGTGGAATATGGCAAGA 778 0.12362176823619703 No Hit GCGTTGATACCACTGCTTCCCATGT 759 0.12060272762374491 No Hit CATGTACTCTGCGTTGATACCACTG 717 0.11392905890148235 No Hit GTCCTACAGTGTGCATTTCTCATTT 688 0.1093210495456344 No Hit GTCCTAAAGTGTGTATTTCTCATTT 679 0.10789097767657813 No Hit GTATCAACGCAGAGTACATGGGAAG 654 0.1039185558180885 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCAAG 40 5.260537E-4 20.108727 1 GACCTAC 45 0.0011614532 17.874424 1 TAACGCA 30 0.008572815 17.873413 4 TGTAGGA 565 0.0 17.265577 2 CCTGTAC 120 0.0 16.45027 3 GTCCAGT 50 0.0023508007 16.08698 1 CTGTAGG 600 0.0 15.86355 1 GTATAGA 60 4.3842473E-4 15.6401205 1 GTCCAGA 60 4.3842473E-4 15.6401205 1 GATATAC 250 0.0 15.550748 1 TAGGACC 260 0.0 15.261146 4 GTCCTAA 265 0.0 15.176396 1 GTAGGAC 620 0.0 14.858309 3 TGTAGAA 270 0.0 14.757249 2 TCCAACG 140 0.0 14.565445 18 GTCTTAC 75 1.4605824E-4 14.299539 1 GTCCTAC 760 0.0 14.111387 1 TGGATTG 45 0.006202993 13.9045105 5 CGTGTAA 40 0.006449218 13.82353 9 GCTTGTG 45 0.007178469 13.601232 7 >>END_MODULE