FastQCFastQC Report
Thu 26 May 2016
SRR1926513_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926513_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629339
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC57030.9061888743586525No Hit
GTACATGGGAAGCAGTGGTATCAAC52900.8405644652564039No Hit
GAGTACATGGGAAGCAGTGGTATCA24490.38913844525764335No Hit
CTGTAGGACGTGGAATATGGCAAGA22780.3619670797455743No Hit
GTCCTACAGTGGACATTTCTAAATT22000.3495731235470867No Hit
CATGTACTCTGCGTTGATACCACTG20610.32748645801388443No Hit
GCGTTGATACCACTGCTTCCCATGT17270.27441490198446306No Hit
ACGCAGAGTACATGGGAAGCAGTGG15870.25216933957692117No Hit
CTTTAGGACGTGAAATATGGCGAGG15250.2423177333678669No Hit
GTATCAACGCAGAGTACATGGGAAG14920.2370741365146606No Hit
GTCCTACAGTGTGCATTTCTCATTT14820.23548516777126474No Hit
GTCCTAAAGTGTGTATTTCTCATTT14480.23008267404371888No Hit
GTATCAACGCAGAGTACTTTTTTTT13510.21466967723277916No Hit
CTGTAGGACCTGGAATATGGCGAGA12170.19337749607127477No Hit
ACTCTGCGTTGATACCACTGCTTCC12030.1911529398305206No Hit
GATATACACTGTTCTACAAATCCCG11720.18622713672599348No Hit
GCTTCCCATGTACTCTGCGTTGATA9820.15603673060147233No Hit
TATCAACGCAGAGTACATGGGAAGC9790.15556003997845358No Hit
GGTATCAACGCAGAGTACATGGGAA9640.1531765868633598No Hit
CTGAAGGACCTGGAATATGGCGAGA9590.1523821024916619No Hit
GGTATCAACGCAGAGTACTTTTTTT8740.13887586817279718No Hit
GTGTATATCAATGAGTTACAATGAA8600.13665131193204297No Hit
CAGTGGTATCAACGCAGAGTACATG8210.13045433383279917No Hit
GTTCTACAGTGTGGTTTTTATCATT7100.11281678078110524No Hit
GTGTATATCAATGAGTTACAATGAG6840.10868546204827603No Hit
GATATACACTGTTCTACAATGCCGG6820.10836766829959688No Hit
CATGGGAAGCAGTGGTATCAACGCA6730.10693759643054061No Hit
TATCAACGCAGAGTACTTTTTTTTT6660.10582531831016354No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6630.10534862768714476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT651.03791535E-817.48492219
TAATACT704.2182E-714.9913914
TAGGACC4850.014.752544
ACTGTTC4000.014.7327248
TCTTAAC400.005126784614.3111092
GTAATAC400.005126784614.3111093
GTTCTAG400.00515134514.3008081
GCCGTGC400.00525613814.2574768
TCCAACG1800.014.20649918
GTCCTAT551.8989947E-413.8674511
TGTAGGA13000.013.5038162
CTGTAGG12700.013.3624341
GCCTAAT500.001458159513.3474211
GTAGGAC12800.013.1930533
CACTGTT4350.013.1092777
GATATAC4450.013.0689021
CCGGTTT1251.05501385E-1012.89182712
TGTCGAG604.192753E-412.62799918
GCCGGTT1302.0372681E-1012.395987511
TAGAAGT701.0470261E-412.2656834
Produced by FastQC (version 0.11.5)