FastQCFastQC Report
Thu 26 May 2016
SRR1926512_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926512_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773037
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17180.2222403326102114No Hit
CTGTAGGACGTGGAATATGGCAAGA13370.17295420529677105No Hit
GTCCTAAAGTGTGTATTTCTCATTT11370.14708222245507005No Hit
CTTTAGGACGTGAAATATGGCGAGG9270.11991664047128404No Hit
GTCCTACAGTGTGCATTTCTCATTT8600.11124952621931422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGC406.801549E-620.4485747
AGGACGG656.344999E-817.7059635
CTGTAGG9250.017.5124721
TGTAGGA9300.017.4221652
GGACGGG701.4852412E-716.4338846
TAGGACC3650.016.241744
GGGTAGG500.002373379416.065161
GTCTTAC604.4326592E-415.6189061
TTAGGGT505.7617924E-415.0900534
CCCTGTA656.715299E-414.787262
GTAGGAC9900.014.7723353
TCTATAC601.18274125E-414.6612633
TATAGGA751.1920906E-414.6464282
AGGACCT6450.014.1123655
TAAGTAG450.00607202113.9501525
TTAGGCA703.3317803E-513.85821154
GTCCTAC10500.013.7701371
TAGGGGT550.001185507313.7182294
GGTATCA2650.013.640231
CACCGGG450.007073207313.6323817
Produced by FastQC (version 0.11.5)