FastQCFastQC Report
Thu 26 May 2016
SRR1926512_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926512_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773037
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA40210.5201562150323982No Hit
GTCCTACAGTGGACATTTCTAAATT38280.4951897515901567No Hit
CTTTAGGACGTGAAATATGGCGAGG28470.3682876757516134No Hit
GTCCTAAAGTGTGTATTTCTCATTT27640.35755080287230756No Hit
GTCCTACAGTGTGCATTTCTCATTT21040.2721732594946943No Hit
CTGTAGGACCTGGAATATGGCGAGA18210.2355644037736874No Hit
CTGAAGGACCTGGAATATGGCGAGA16810.21745401578449672No Hit
GTATCAACGCAGAGTACTTTTTTTT16780.21706593604187124No Hit
GATATACACTGTTCTACAAATCCCG14220.18394979800449396No Hit
GTGTATATCAATGAGTTACAATGAA10920.14126102631568735No Hit
GAATATGGCAAGAAAACTGAAAATC10420.1347930306052621No Hit
GGTATCAACGCAGAGTACTTTTTTT10380.1342755909484281No Hit
ACCTGGAATATGGCGAGAAAACTGA9660.12496167712541574No Hit
GTCCTTCAGTGTGCATTTCTCATTT9430.12198639909862012No Hit
GTTCTACAGTGTGGTTTTTATCATT9010.11655328270186291No Hit
CTGTAGGACATGGAATATGGCAAGA8850.11448352407452685No Hit
ACAGTGGACATTTCTAAATTTTCCA8640.11176696587614823No Hit
GCCATATTCCACGTCCTACAGTGGA8560.11073208656248018No Hit
GTGTATATCAATGAGTTACAATGAG8520.11021464690564617No Hit
GACCTGGAATATGGCGAGAAAACTG8350.10801552836410158No Hit
ATTCCAGGTCCTTCAGTGTGCATTT8030.10387601110942943No Hit
GATATACACTGTTCTACAATGCCGG8030.10387601110942943No Hit
GGAATATGGCGAGAAAACTGAAAAT7930.10258241196734437No Hit
TATCAACGCAGAGTACTTTTTTTTT7770.1005126533400083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1850.016.38347818
GTCCTAT508.406163E-515.2623321
CCAACGA1250.015.15373819
TCGTCAA456.5212965E-414.8470832
CTAGGTC653.2180487E-614.6791444
CCAACGT851.7822458E-814.48519119
CGTTTCC1257.2759576E-1213.65072614
TAGGACC6400.013.4176564
GTATAAG801.9060153E-613.1160671
CTTACAC956.936352E-813.0601183
GTAGATC603.9317703E-412.7252433
TGTACTG905.1157076E-712.7219265
CTCTAGG1750.012.5369161
GGCGAGG7900.012.34837819
GACAGAT853.950485E-612.2900897
CTAGACT701.0461235E-412.2675714
GATATAC5600.012.2643741
GTCTTAC701.048673E-412.2643741
TTCTAGC550.00296041912.14761452
ACAGTCC951.0391741E-611.9984038
Produced by FastQC (version 0.11.5)