FastQCFastQC Report
Thu 26 May 2016
SRR1926511_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926511_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534492
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC19300.36109053082178966No Hit
CCCATGTACTCTGCGTTGATACCAC17880.33452324824319163No Hit
GAGTACATGGGAAGCAGTGGTATCA9340.1747453656930319No Hit
GTCCTACAGTGGACATTTCTAAATT8840.16539068872873683No Hit
CATGTACTCTGCGTTGATACCACTG7180.1343331612072772No Hit
CTGTAGGACGTGGAATATGGCAAGA6760.12647523255726933No Hit
GCGTTGATACCACTGCTTCCCATGT5840.10926262694296641No Hit
ACGCAGAGTACATGGGAAGCAGTGG5760.1077658786286792No Hit
GTATCAACGCAGAGTACATGGGAAG5420.10140469829295853No Hit
GTCCTACAGTGTGCATTTCTCATTT5390.10084341767510084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGTG304.8460424E-420.3559467
TTGTAGA801.9463187E-920.0004921
GTCCAGG450.001203745817.7782151
CTGTAGG4100.017.5614071
GATATAC2000.017.3337611
TGTAGGA4900.016.9918752
TGTAGAA2500.016.9250222
TAGGACC2500.016.2793944
GGACCAG453.3928195E-416.1765846
CTGTCCA350.002722439115.7382189
TCCTAGA801.2609466E-515.3553622
TCCTGGG550.003933056714.8900492
TCTAGGA657.01781E-414.699152
TAGAACA2700.014.6768434
TATGCTG507.517433E-414.5682425
GTGTAGA1109.931318E-814.5458121
GTAGAAC2750.014.2896493
CAAGACT450.005195962314.2801714
GTAGGAC5050.014.0499173
GTCCTAA2300.013.9133841
Produced by FastQC (version 0.11.5)