FastQCFastQC Report
Thu 26 May 2016
SRR1926511_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926511_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534492
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC51920.9713896559723999No Hit
GTACATGGGAAGCAGTGGTATCAAC48780.9126422846366269No Hit
GAGTACATGGGAAGCAGTGGTATCA23260.4351795723790066No Hit
CATGTACTCTGCGTTGATACCACTG19970.37362579795394507No Hit
CTGTAGGACGTGGAATATGGCAAGA19110.35753575357535755No Hit
GTCCTACAGTGGACATTTCTAAATT18160.3397618673431969No Hit
GCGTTGATACCACTGCTTCCCATGT16250.30402700133958976No Hit
ACGCAGAGTACATGGGAAGCAGTGG15150.2834467120181406No Hit
GTATCAACGCAGAGTACATGGGAAG14670.27446622213241734No Hit
GTATCAACGCAGAGTACTTTTTTTT12780.23910554320738195No Hit
CTTTAGGACGTGAAATATGGCGAGG12380.2316218016359459No Hit
GTCCTAAAGTGTGTATTTCTCATTT11590.2168414120323597No Hit
ACTCTGCGTTGATACCACTGCTTCC11580.2166543184930738No Hit
GTCCTACAGTGTGCATTTCTCATTT11350.21235116708949808No Hit
GCTTCCCATGTACTCTGCGTTGATA10400.19457728085733744No Hit
GATATACACTGTTCTACAAATCCCG10240.19158378422876302No Hit
CTGTAGGACCTGGAATATGGCGAGA9970.18653225866804368No Hit
TATCAACGCAGAGTACATGGGAAGC9460.1769904881644627No Hit
GGTATCAACGCAGAGTACATGGGAA8740.16351975333587782No Hit
GGTATCAACGCAGAGTACTTTTTTT8410.15734566653944304No Hit
CTGAAGGACCTGGAATATGGCGAGA7560.14144271570014144No Hit
CAGTGGTATCAACGCAGAGTACATG7270.13601700306085032No Hit
TATCAACGCAGAGTACTTTTTTTTT6840.12797198087155653No Hit
GTTCTACAGTGTGGTTTTTATCATT6300.11786892975011788No Hit
GTGGTATCAACGCAGAGTACATGGG6250.11693346205368836No Hit
GTGTATATCAATGAGTTACAATGAA6200.11599799435725885No Hit
CATGGGAAGCAGTGGTATCAACGCA6150.11506252666082936No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6130.11468833958225755No Hit
GTGTATATCAATGAGTTACAATGAG5990.11206903003225493No Hit
ACATGGGAAGCAGTGGTATCAACGC5820.1088884398643946No Hit
ATACCACTGCTTCCCATGTACTCTG5450.10196597891081625No Hit
GCAGTGGTATCAACGCAGAGTACAT5420.10140469829295853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1150.017.3017818
CCAACGA855.820766E-1116.7202119
ACCGTGC350.002161399516.2927938
TTAGAAC456.5686245E-414.8315293
TAACACC456.5686245E-414.8315294
CTAGGAC801.2272722E-714.3018313
GTACTAA551.9030663E-413.8632221
GTGCGCC551.9898763E-413.79053711
GTCGAGG552.0009675E-413.78150619
TAGGACC3900.013.6906414
GTCCTAG707.0016904E-613.61566451
GTCTTAC707.0016904E-613.61566451
ATGTCGA707.435561E-613.53667417
TGTCGAG707.4427517E-613.53540718
TGTAGGA9100.013.0993812
ACTGTTC3350.013.05044658
GGCGAGG3450.012.90767819
GGACGTG7850.012.6317656
GTCCTAC9750.012.5124451
GTAGGAC9550.012.4797833
Produced by FastQC (version 0.11.5)