FastQCFastQC Report
Thu 26 May 2016
SRR1926510_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926510_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683922
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15170.22180891973061254No Hit
CTGTAGGACGTGGAATATGGCAAGA12040.17604346694506098No Hit
GTCCTAAAGTGTGTATTTCTCATTT9840.1438760560414784No Hit
GTCCTACAGTGTGCATTTCTCATTT8870.12969315214308064No Hit
CTTTAGGACGTGAAATATGGCGAGG8090.11828834282271955No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGT250.003037597821.3871384
CTAGGGC452.3321496E-416.992833
GTCCTAC10350.016.7184721
AATGGCG352.9568476E-416.4738116
GTCCTAG1003.1575837E-815.9725691
GTCTAGC500.002466110315.9725691
TCCTACA11550.015.5668062
GGTATCA1550.015.4573271
GATATAC4250.015.3461951
TGTAGGA8550.015.2937032
CTAGAAC505.202941E-415.2935473
TATTAGC550.00399036314.8592222
TAGGACC3700.014.7397844
ATATACA4650.014.6461882
TTTAGGA4850.014.60390952
CCCTTAT550.004651350414.5205181
GTCCAAG658.4975327E-414.3343581
CTGTAGG9150.014.2560271
TCCTAGA1252.3772373E-814.1657922
AGGACGG450.006391322213.8422565
Produced by FastQC (version 0.11.5)