##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926510_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 683922 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.631025175385496 27.0 2.0 30.0 2.0 31.0 2 16.09927301651358 16.0 2.0 30.0 2.0 31.0 3 19.603520576907894 23.0 12.0 31.0 2.0 31.0 4 21.43524700185109 27.0 2.0 35.0 2.0 35.0 5 21.863053096698163 28.0 2.0 35.0 2.0 35.0 6 22.395232204842074 29.0 2.0 35.0 2.0 36.0 7 22.590696015042653 30.0 2.0 35.0 2.0 37.0 8 23.019145458107797 30.0 2.0 35.0 2.0 37.0 9 25.562257099493802 31.0 17.0 37.0 2.0 39.0 10 26.210816438131832 31.0 18.0 37.0 2.0 39.0 11 23.396953161325413 30.0 7.0 37.0 2.0 39.0 12 24.44913893689631 30.0 8.0 37.0 2.0 39.0 13 21.21726307970792 27.0 2.0 35.0 2.0 39.0 14 24.419540532399893 30.0 10.0 37.0 2.0 40.0 15 25.521034562420862 31.0 10.0 38.0 2.0 40.0 16 25.872128693038096 31.0 8.0 38.0 2.0 40.0 17 25.14746564666731 31.0 8.0 38.0 2.0 40.0 18 25.201362143636263 31.0 9.0 38.0 2.0 40.0 19 25.604030869017226 31.0 8.0 38.0 2.0 40.0 20 25.852869186837097 31.0 2.0 38.0 2.0 40.0 21 23.69939262079594 30.0 2.0 37.0 2.0 40.0 22 24.622766923713524 30.0 2.0 38.0 2.0 40.0 23 24.597673418898648 31.0 2.0 38.0 2.0 40.0 24 23.741371092025116 31.0 2.0 38.0 2.0 40.0 25 23.515734250396974 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.462917147646515 1101 2 9.983759395808436 1101 3 6.927309642434363 1101 4 8.282174908697868 1101 5 7.929071173350152 1101 6 7.1796594884106035 1101 7 7.244923081102417 1101 8 5.746249607513491 1101 9 5.580776584508683 1101 10 5.245212280822223 1101 11 7.960468790122633 1101 12 5.510576708718158 1101 13 10.102801628605935 1101 14 7.600853919461596 1101 15 6.870644135109469 1101 16 6.3723488147452265 1101 17 2.146685497482565 1101 18 4.60714382764078 1101 19 5.224087799633654 1101 20 3.44770020701678 1101 21 0.7753163262663421 1101 22 4.4116416309094255 1101 23 5.51397731090319 1101 24 6.837133291065818 1101 25 7.361858826366397 1102 1 5.428306983994489 1102 2 10.171585752397581 1102 3 6.622196808670367 1102 4 7.901778698534219 1102 5 7.910808296519832 1102 6 6.223469996593206 1102 7 6.433671401515852 1102 8 6.982643750493679 1102 9 5.935421890279571 1102 10 5.245209524577607 1102 11 7.908153200114022 1102 12 5.362181876676814 1102 13 9.662255892171828 1102 14 7.338206546507244 1102 15 5.4979516286495205 1102 16 5.591426161859783 1102 17 6.604202810144063 1102 18 6.661731941335869 1102 19 5.150207351743905 1102 20 5.510783066620569 1102 21 4.5018748103318025 1102 22 5.51705145003087 1102 23 6.048673607199486 1102 24 7.624595134304403 1102 25 7.203203184678198 1103 1 4.322374902573678 1103 2 8.672671820829857 1103 3 5.817033721268981 1103 4 7.389218610497355 1103 5 6.496791481833441 1103 6 5.6998034472795 1103 7 5.888890176218098 1103 8 5.60698585430012 1103 9 4.662053705331303 1103 10 4.099479290248102 1103 11 4.607645482496242 1103 12 3.9636180112031596 1103 13 7.718552099899853 1103 14 5.988138238227659 1103 15 5.009194263644172 1103 16 4.8125147102036685 1103 17 2.6641723869427203 1103 18 1.26506122267077 1103 19 3.5235270455633874 1103 20 4.039613146177018 1103 21 6.2421173545439785 1103 22 1.9398143815520967 1103 23 4.0874244402947895 1103 24 5.384991388752191 1103 25 1.1871523996311808 1104 1 5.573908875140432 1104 2 10.189598163042822 1104 3 6.81643535208562 1104 4 7.038930788665429 1104 5 7.304245869213897 1104 6 6.661080412984813 1104 7 6.839092099756108 1104 8 6.997474911649743 1104 9 6.011457525303491 1104 10 5.081538639297488 1104 11 7.833286957194815 1104 12 4.5694094743304845 1104 13 9.255420606708125 1104 14 6.833456676963621 1104 15 6.033589796098926 1104 16 5.566384824477588 1104 17 6.042008125219791 1104 18 3.908303924964379 1104 19 4.303466875059446 1104 20 3.6379010180467866 1104 21 2.3561786133709646 1104 22 4.726598970730997 1104 23 5.107608454455502 1104 24 6.320936697391851 1104 25 5.24231494964296 1105 1 2.9560307952891947 1105 2 8.683694548827459 1105 3 5.756189708604751 1105 4 4.955326273462138 1105 5 1.5706420583791 1105 6 3.815735584357668 1105 7 5.283896778372888 1105 8 2.1711118076789155 1105 9 3.080032829339121 1105 10 2.9817376737089063 1105 11 6.13599434843529 1105 12 3.4523454432094702 1105 13 4.470842654246962 1105 14 3.338009916153073 1105 15 3.9867533162509083 1105 16 4.097698028971859 1105 17 4.640493934207132 1105 18 1.8086981700725424 1105 19 1.928795161089404 1105 20 0.7822120349737673 1105 21 1.7896369217998398 1105 22 3.378023103200576 1105 23 1.0633781380330483 1105 24 4.0423448708838485 1105 25 4.575535584017349 1106 1 6.015564309916698 1106 2 10.71555987542474 1106 3 7.464419154824451 1106 4 8.552966275604344 1106 5 8.093570613797791 1106 6 7.175681870504928 1106 7 6.932156334499698 1106 8 6.998717713029077 1106 9 5.87442262767415 1106 10 5.133292856361791 1106 11 7.3195140339275895 1106 12 4.970606764673391 1106 13 4.456392675768207 1106 14 5.448561673258556 1106 15 5.427050210249359 1106 16 5.276602052155297 1106 17 5.7119305814154195 1106 18 5.789053340030868 1106 19 5.628993315221184 1106 20 5.195171989590719 1106 21 5.089361410199196 1106 22 5.71726337351534 1106 23 6.123280588281286 1106 24 6.621986368603785 1106 25 5.901869058180946 1107 1 3.062046838857917 1107 2 -13.533840604191566 1107 3 -5.684715694066034 1107 4 1.0548431588958103 1107 5 3.443128497182297 1107 6 3.952669781363891 1107 7 3.8050770003423224 1107 8 4.267935118202324 1107 9 2.7601634411991007 1107 10 2.181755748755716 1107 11 4.7460212841843905 1107 12 2.1950333991377917 1107 13 3.4570781131665065 1107 14 2.753087648677745 1107 15 2.5838919577539663 1107 16 2.2811931536209187 1107 17 2.9911675244025986 1107 18 -1.9752834090971447 1107 19 0.8202702224365055 1107 20 1.0566737620444897 1107 21 -2.337656595348861 1107 22 0.7080204115903435 1107 23 -2.0663083581387767 1107 24 1.1626009078512496 1107 25 1.8845717321462878 1108 1 4.929907332682717 1108 2 10.142748293636272 1108 3 -0.20420121838623473 1108 4 5.49752486847261 1108 5 6.9334785747982615 1108 6 7.011382738611729 1108 7 6.997053732751652 1108 8 6.820374144922983 1108 9 5.23607956117803 1108 10 4.266192489993582 1108 11 6.527516899535346 1108 12 2.1119578832957906 1108 13 3.503930510343668 1108 14 4.482288513186454 1108 15 4.521932952486782 1108 16 4.545683006217473 1108 17 0.3447134942645462 1108 18 3.2877700545112525 1108 19 4.239568411057629 1108 20 4.596847109671646 1108 21 4.724557838736175 1108 22 1.6672091288981612 1108 23 4.492522121844459 1108 24 4.965887595168795 1108 25 5.608142334009198 1109 1 3.512689190657266 1109 2 -13.533840604191566 1109 3 -5.4399089955584685 1109 4 2.3665476685544995 1109 5 1.203534255787428 1109 6 2.516383502747523 1109 7 3.721034909059405 1109 8 4.256788675613848 1109 9 3.610291996695061 1109 10 2.9024710621842296 1109 11 1.0920186109111647 1109 12 1.932011834166186 1109 13 2.8609722379249334 1109 14 3.6414976470601594 1109 15 3.6998771100198624 1109 16 3.5358011444943322 1109 17 4.132980837984359 1109 18 4.097986838393467 1109 19 2.7617838569813244 1109 20 1.568054328880578 1109 21 0.6233148925745873 1109 22 3.1521477240993896 1109 23 3.5247787445949434 1109 24 4.5252508537898315 1109 25 4.850142697334139 1110 1 4.125345114527839 1110 2 10.046656175937228 1110 3 7.100619670033257 1110 4 8.413840042092534 1110 5 9.1162152395875 1110 6 4.646161788318608 1110 7 7.329748840072057 1110 8 8.233343627752678 1110 9 6.8887383140395 1110 10 6.483322308421119 1110 11 4.432416536212791 1110 12 6.8011849883869715 1110 13 11.188531895395265 1110 14 8.913980690390755 1110 15 8.506861246425014 1110 16 8.011732071414961 1110 17 8.331762958384132 1110 18 8.328657719085122 1110 19 7.3597838437918846 1110 20 7.669214834431681 1110 21 5.802110070516573 1110 22 7.8272383190419 1110 23 8.478228276864552 1110 24 9.121635958959104 1110 25 9.66350887236456 1111 1 4.960953744757269 1111 2 9.405164211217743 1111 3 7.515836127185565 1111 4 8.377648423946667 1111 5 8.637614351520295 1111 6 7.7124877388118875 1111 7 7.516259196672241 1111 8 7.636016541702894 1111 9 6.266647531539178 1111 10 5.205922393181776 1111 11 6.986421920138685 1111 12 6.427117318670003 1111 13 8.353708851719901 1111 14 5.939594208386154 1111 15 5.545398549234669 1111 16 5.486407241711518 1111 17 6.713710858638265 1111 18 6.614948643050088 1111 19 5.710326001880844 1111 20 5.425750929328174 1111 21 7.812650515672441 1111 22 7.142515467185508 1111 23 7.186566717002709 1111 24 6.840821252542543 1111 25 7.372925279014872 1112 1 -1.8670251409107017 1112 2 7.805297040916228 1112 3 5.815654584391243 1112 4 -0.22067749594557995 1112 5 3.9019507753402003 1112 6 2.983924828377436 1112 7 5.633756248598271 1112 8 5.868260884462078 1112 9 4.66166149329807 1112 10 3.5043273720328365 1112 11 -0.2230191037413789 1112 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5.8500314415757515 2204 5 1.1730376976883221 2204 6 3.84328374118601 2204 7 5.2273524577036135 2204 8 5.406223646796153 2204 9 3.786801520460859 2204 10 3.3202633482859305 2204 11 4.901474426331003 2204 12 5.061122908547361 2204 13 3.2393946260440245 2204 14 4.407762886156725 2204 15 4.458128336646617 2204 16 4.532516876231135 2204 17 -0.8008861506813965 2204 18 2.7848251256766474 2204 19 3.5593506518966755 2204 20 3.9678634862652835 2204 21 5.432223585019546 2204 22 5.13470636190857 2204 23 3.73700583353342 2204 24 5.3663862372656475 2204 25 5.483403489047866 2205 1 6.005523641153008 2205 2 10.169893162042202 2205 3 7.258777174901894 2205 4 7.756460622983585 2205 5 7.754397147376125 2205 6 7.110315332566447 2205 7 7.331677626556964 2205 8 7.249434023097905 2205 9 6.0756454156775135 2205 10 5.675570722380666 2205 11 7.81113372518757 2205 12 7.067429955471404 2205 13 10.079130200034509 2205 14 7.625461711669388 2205 15 6.595358420823754 2205 16 6.111005957602369 2205 17 6.851889393586461 2205 18 6.838281489978442 2205 19 5.524686500932354 2205 20 5.845776830393401 2205 21 -0.658666790616774 2205 22 4.032590981558776 2205 23 5.302701986227863 2205 24 7.00976186249439 2205 25 7.542093891301462 2206 1 4.115626510353046 2206 2 8.444916280151473 2206 3 5.7403733717813346 2206 4 1.4607429653461779 2206 5 5.476329080509867 2206 6 5.9219915104405025 2206 7 6.094887518553243 2206 8 6.105486114201138 2206 9 4.569270054217572 2206 10 3.5857038622541673 2206 11 3.505297743702492 2206 12 4.222181429410526 2206 13 7.466444744262898 2206 14 -0.006439235534472232 2206 15 1.8835033011204843 2206 16 2.847279420567414 2206 17 3.837128927852355 2206 18 -0.8689027498572521 2206 19 0.6239877209554479 2206 20 2.121839624798053 2206 21 2.859721046958711 2206 22 -2.0777456232211797 2206 23 -2.722839054163689 2206 24 1.0793639755662099 2206 25 2.593651745327847 2207 1 1.5905610144214926 2207 2 -13.533840604191566 2207 3 -5.92123300581909 2207 4 -4.368060672130724 2207 5 1.5724609377693213 2207 6 1.9120266695641384 2207 7 3.273479376796974 2207 8 3.4749950177490696 2207 9 1.2073624496589659 2207 10 0.8124038324063036 2207 11 -1.2630457509009254 2207 12 -2.1713521296246547 2207 13 -0.03408350543143257 2207 14 -5.586932920505912 2207 15 -1.5340804790253806 2207 16 -0.4255351820053832 2207 17 0.6021603959391051 2207 18 1.0419064596472793 2207 19 0.4416351595036794 2207 20 0.14497331830841276 2207 21 -3.640230058786461 2207 22 -4.292114014906986 2207 23 -1.469393607049696 2207 24 0.23748032599676705 2207 25 0.5945938385516136 2208 1 5.157581663775545 2208 2 6.643578750647142 2208 3 6.4206644811440405 2208 4 8.388949102246258 2208 5 4.012226012059827 2208 6 6.479465940023509 2208 7 7.014852113360483 2208 8 6.970946381707037 2208 9 5.387621745799006 2208 10 4.793689700177062 2208 11 6.395423628832312 2208 12 6.338821870008481 2208 13 8.906491951186581 2208 14 3.284866822687402 2208 15 4.924160264141726 2208 16 5.291535911519421 2208 17 6.2271564652245 2208 18 0.8211051179633593 2208 19 3.6778232835046225 2208 20 4.639738916694199 2208 21 7.473942132717955 2208 22 6.535570663167491 2208 23 2.266086988322545 2208 24 3.565752645904528 2208 25 4.912239471527688 2209 1 3.299050254607465 2209 2 4.922865677642049 2209 3 5.479493004064242 2209 4 0.5192547049627265 2209 5 5.093083397458809 2209 6 5.814864921347787 2209 7 6.498341077706833 2209 8 4.905581356240145 2209 9 4.798699844270992 2209 10 3.962365421739033 2209 11 -0.3009261164987578 2209 12 3.335001836052623 2209 13 2.0003798968572077 2209 14 3.9610128328741574 2209 15 4.452470960236802 2209 16 3.893658152606008 2209 17 1.225840675750817 2209 18 3.59275197704655 2209 19 4.434911568733824 2209 20 4.362574230785878 2209 21 6.458959110646649 2209 22 5.333575756546097 2209 23 0.8508492973208455 2209 24 -3.087558050190548 2209 25 0.02161977713040386 2210 1 -2.2955189534365203 2210 2 -13.533840604191566 2210 3 -7.645080249262389 2210 4 -2.495731228486246 2210 5 -0.9427786461444327 2210 6 -1.4633080206146651 2210 7 -1.3223057997640097 2210 8 -20.41615039248651 2210 9 -12.975063126682654 2210 10 -7.1640021596109875 2210 11 -9.839635180996499 2210 12 -4.962039897780038 2210 13 -5.36256010424605 2210 14 -4.244363409058263 2210 15 -5.81896597319378 2210 16 -5.31479342352192 2210 17 -4.772328220928987 2210 18 -4.7572691687491115 2210 19 -9.847415449463819 2210 20 -7.0363994319723915 2210 21 -4.916558041964706 2210 22 -10.06241757486675 2210 23 -7.9380559020932004 2210 24 -10.621233495937794 2210 25 -8.274871859554178 2211 1 -0.8307759407174409 2211 2 3.8764831053447413 2211 3 -0.11281984138016199 2211 4 0.6650970434310288 2211 5 0.0398309283807734 2211 6 -0.8567079656576375 2211 7 -20.026276918897583 2211 8 -13.496640331244166 2211 9 -9.745188769822027 2211 10 -8.066075208241642 2211 11 -9.920300413726885 2211 12 -5.71185828690739 2211 13 -7.213591022312698 2211 14 -5.507297261640769 2211 15 -5.945495847392717 2211 16 -12.12776107151899 2211 17 -8.706342673995998 2211 18 -6.651702716541198 2211 19 -9.435962594067133 2211 20 -7.51496855938742 2211 21 -5.287791985194726 2211 22 -6.686004563316299 2211 23 -8.987780169411772 2211 24 -21.311666708934183 2211 25 -21.0853411736336 2212 1 -0.47243467142919826 2212 2 -13.533840604191566 2212 3 -7.29421641459612 2212 4 -18.95782509130213 2212 5 -19.28212882664985 2212 6 -19.796340511589396 2212 7 -9.12657190414832 2212 8 -8.58527526957599 2212 9 -4.270579954331044 2212 10 -4.751238456640902 2212 11 -4.87688106238474 2212 12 -2.1442323374961987 2212 13 -5.0749556968611245 2212 14 -2.8557824620526624 2212 15 -2.995542688882665 2212 16 -3.0813841252744396 2212 17 -3.166326105270631 2212 18 -2.166239063214679 2212 19 -2.835965691025148 2212 20 -2.9882069709576555 2212 21 -1.007184362032575 2212 22 -2.186728477251833 2212 23 -2.2358232790673114 2212 24 -1.305767003919435 2212 25 -1.792322491234387 2213 1 -18.175482852353486 2213 2 -13.533840604191566 2213 3 -8.219358314971252 2213 4 -5.153372041931366 2213 5 -19.28212882664985 2213 6 -9.762458614203142 2213 7 -20.026276918897583 2213 8 -10.151871592873732 2213 9 -7.65756843001213 2213 10 -6.657565647541766 2213 11 -5.709361994001277 2213 12 -4.412926873082423 2213 13 -18.554798371394064 2213 14 -11.67957279883463 2213 15 -6.403206784542032 2213 16 -5.495912172670067 2213 17 -4.104415373766223 2213 18 -4.117376598303075 2213 19 -4.892548792815525 2213 20 -4.779717411569862 2213 21 -2.9873911277510814 2213 22 -3.7541062877740217 2213 23 -3.5912819340145283 2213 24 -2.719217531780263 2213 25 -3.642940399190234 2214 1 -18.175482852353486 2214 2 -13.533840604191566 2214 3 -12.461456315012446 2214 4 -18.95782509130213 2214 5 -19.28212882664985 2214 6 -19.796340511589396 2214 7 -16.192234365706092 2214 8 -15.067214222273744 2214 9 -14.114831926129616 2214 10 -14.277689846837351 2214 11 -14.648399294983749 2214 12 -12.816291376388227 2214 13 -18.554798371394064 2214 14 -16.552916293304364 2214 15 -23.06535586489053 2214 16 -18.065361823552646 2214 17 -22.731714502517434 2214 18 -17.587885959593265 2214 19 -15.172090923770602 2214 20 -13.988504048302369 2214 21 -11.610666652457063 2214 22 -12.570451986111852 2214 23 -12.50506949760745 2214 24 -11.3871986238278 2214 25 -11.16938372682509 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 136624.0 3 6.0 4 564.0 5 2266.0 6 2938.0 7 2929.0 8 2569.0 9 2635.0 10 2315.0 11 2244.0 12 2123.0 13 2284.0 14 3508.0 15 6837.0 16 9956.0 17 10852.0 18 11239.0 19 12813.0 20 15172.0 21 17514.0 22 18509.0 23 20440.0 24 21031.0 25 21039.0 26 17883.0 27 15044.0 28 16322.0 29 18350.0 30 18023.0 31 20898.0 32 26320.0 33 32662.0 34 38556.0 35 43009.0 36 41138.0 37 40689.0 38 26621.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.592402213170146 18.513643556914303 13.33783877159956 25.556115458315986 2 12.575335956098385 20.349504004971372 44.25938872211027 22.815771316819966 3 19.83389569791098 23.627840768123853 29.403190591971416 27.135072941993748 4 11.898741879001346 16.828804954062697 38.645305876865045 32.627147290070916 5 12.178659496792292 37.589349158366794 36.34778616626074 13.884205178580169 6 31.024093295258286 37.339990766231764 17.935739812930695 13.700176125579247 7 27.174175479260228 30.280876269576833 22.632188903375344 19.912759347787595 8 22.809780918906377 41.670388777795495 19.76922384907852 15.750606454219612 9 25.627367632162912 16.154204212116422 20.073129460809284 38.14529869491138 10 14.593452539809626 30.135219286540345 34.680633395605376 20.590694778044657 11 33.5934725326195 21.805010367829176 25.498656137025943 19.102860962525376 12 24.572779863623843 23.815375282053505 32.7660279128505 18.845816941472158 13 29.09750595290897 21.70894145190102 24.962212725397396 24.231339869792617 14 19.20259407839842 24.025513188643778 27.551096158471584 29.22079657448622 15 21.591584676260183 34.36357533861022 23.037084196885342 21.007755788244253 16 20.29599392214558 28.383067628189668 30.9126429019343 20.40829554773045 17 19.862474703019085 29.171763679177857 30.613371852453714 20.352389765349344 18 19.782792192861308 27.928010787700952 33.515749123647645 18.773447895790095 19 23.04503195385596 26.30047004047983 26.826411233522634 23.828086772141578 20 22.89747447640332 28.922099138019526 28.76562871195657 19.414797673620583 21 21.13725688027113 26.41966023239212 26.53538239525004 25.907700492086715 22 21.630262558043874 30.14494819672326 28.288880672039298 19.93590857319357 23 21.23705454838214 28.997372485014385 28.959899290790524 20.805673675812955 24 22.847196451607203 27.2733698646862 29.752810938200327 20.126622745506275 25 21.997045902690456 28.153659904057708 29.761871650473783 20.087422542778054 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 737.0 1 737.0 2 1543.5 3 2350.0 4 2350.0 5 2350.0 6 3222.5 7 4095.0 8 4095.0 9 4095.0 10 4797.0 11 5499.0 12 5499.0 13 5499.0 14 6941.0 15 8383.0 16 8383.0 17 8383.0 18 12097.0 19 15811.0 20 15811.0 21 15811.0 22 22310.5 23 28810.0 24 28810.0 25 28810.0 26 38385.0 27 47960.0 28 47960.0 29 47960.0 30 59173.0 31 70386.0 32 70386.0 33 70386.0 34 75665.0 35 80944.0 36 80944.0 37 80944.0 38 82869.5 39 84795.0 40 84795.0 41 84795.0 42 84274.5 43 83754.0 44 83754.0 45 83754.0 46 80920.0 47 78086.0 48 78086.0 49 78086.0 50 70862.5 51 63639.0 52 63639.0 53 63639.0 54 54235.0 55 44831.0 56 44831.0 57 44831.0 58 37266.0 59 29701.0 60 29701.0 61 29701.0 62 23898.0 63 18095.0 64 18095.0 65 18095.0 66 13720.5 67 9346.0 68 9346.0 69 9346.0 70 6738.5 71 4131.0 72 4131.0 73 4131.0 74 2933.0 75 1735.0 76 1735.0 77 1735.0 78 1156.0 79 577.0 80 577.0 81 577.0 82 384.5 83 192.0 84 192.0 85 192.0 86 121.0 87 50.0 88 50.0 89 50.0 90 31.5 91 13.0 92 13.0 93 13.0 94 7.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.24200712946798 2 36.000596559256756 3 5.706206263287334 4 13.487795391872174 5 13.370384342074097 6 14.491711043072165 7 9.160693763323888 8 7.357710382178084 9 0.10966162808039515 10 1.3834910998622652 11 5.722728615251447 12 5.522267159120484 13 15.936466439155343 14 1.5188866566655261 15 2.8533955626518814 16 3.001804299320683 17 2.824444892838657 18 1.4359824658367475 19 1.3908018750676245 20 0.29301587023081577 21 4.352689341767044 22 1.5365787326624964 23 0.11214729165021742 24 8.983919218858292 25 0.1174110497980764 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 683922.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.07440229518919 #Duplication Level Percentage of deduplicated Percentage of total 1 96.58469906080087 86.03224339701558 2 2.298956412390109 4.095563366726829 3 0.4378774305865844 1.170110112241596 4 0.21313551520275928 0.7593967449825199 5 0.11460872255170962 0.5104351729554351 6 0.0724473822654621 0.38719243638882744 7 0.050190879278854975 0.31295058007038024 8 0.033803026893359144 0.24087875330353375 9 0.019287966266041706 0.15462576599736935 >10 0.14860156905631813 2.4705525364553815 >50 0.014909948057355732 0.9082902589463916 >100 0.010111091230164152 1.8595914007687377 >500 0.0010282465653331315 0.6828067914736132 >1k 3.427488551110438E-4 0.415362682673754 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1517 0.22180891973061254 No Hit CTGTAGGACGTGGAATATGGCAAGA 1204 0.17604346694506098 No Hit GTCCTAAAGTGTGTATTTCTCATTT 984 0.1438760560414784 No Hit GTCCTACAGTGTGCATTTCTCATTT 887 0.12969315214308064 No Hit CTTTAGGACGTGAAATATGGCGAGG 809 0.11828834282271955 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATAGT 25 0.0030375978 21.387138 4 CTAGGGC 45 2.3321496E-4 16.99283 3 GTCCTAC 1035 0.0 16.718472 1 AATGGCG 35 2.9568476E-4 16.47381 16 GTCCTAG 100 3.1575837E-8 15.972569 1 GTCTAGC 50 0.0024661103 15.972569 1 TCCTACA 1155 0.0 15.566806 2 GGTATCA 155 0.0 15.457327 1 GATATAC 425 0.0 15.346195 1 TGTAGGA 855 0.0 15.293703 2 CTAGAAC 50 5.202941E-4 15.293547 3 TATTAGC 55 0.003990363 14.859222 2 TAGGACC 370 0.0 14.739784 4 ATATACA 465 0.0 14.646188 2 TTTAGGA 485 0.0 14.6039095 2 CCCTTAT 55 0.0046513504 14.520518 1 GTCCAAG 65 8.4975327E-4 14.334358 1 CTGTAGG 915 0.0 14.256027 1 TCCTAGA 125 2.3772373E-8 14.165792 2 AGGACGG 45 0.0063913222 13.842256 5 >>END_MODULE