FastQCFastQC Report
Thu 26 May 2016
SRR1926510_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926510_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683922
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA34420.5032737651369601No Hit
GTCCTACAGTGGACATTTCTAAATT33480.4895295077508839No Hit
CTTTAGGACGTGAAATATGGCGAGG23710.3466769602381558No Hit
GTCCTAAAGTGTGTATTTCTCATTT21680.31699521290439553No Hit
GTCCTACAGTGTGCATTTCTCATTT21330.3118776702606438No Hit
GATATACACTGTTCTACAAATCCCG17580.2570468562204462No Hit
CTGTAGGACCTGGAATATGGCGAGA16710.24432610736312035No Hit
GTATCAACGCAGAGTACTTTTTTTT15930.23292129804275927No Hit
CTGAAGGACCTGGAATATGGCGAGA13530.19782957705703283No Hit
GTGTATATCAATGAGTTACAATGAA12890.18847178479417243No Hit
GTGTATATCAATGAGTTACAATGAG10480.15323384830433878No Hit
GGTATCAACGCAGAGTACTTTTTTT9850.1440222715455856No Hit
GATATACACTGTTCTACAATGCCGG9640.14095174595933455No Hit
GTTCTACAGTGTGGTTTTTATCATT9610.14051309944701296No Hit
CTGTAGGACATGGAATATGGCAAGA8430.12325966996236412No Hit
TTGTAGAACAGTGTATATCAATGAG8410.12296723895414974No Hit
GTTCTACAAATCCCGTTTCCAACGA8310.12150508391307782No Hit
CTGTAGAACATATTAGATGAGTGAG7960.11638754126932604No Hit
GAATATGGCAAGAAAACTGAAAATC7910.11565646374879007No Hit
TATCAACGCAGAGTACTTTTTTTTT7870.1150716017323613No Hit
ACCTGGAATATGGCGAGAAAACTGA7780.11375566219539657No Hit
GTCCTTCAGTGTGCATTTCTCATTT7400.1081994730393232No Hit
ATTCCAGGTCCTTCAGTGTGCATTT7390.108053257535216No Hit
GACCTGGAATATGGCGAGAAAACTG7030.10278949938735704No Hit
ACAGTGGACATTTCTAAATTTTCCA7010.10249706837914266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1750.015.70275619
TCCAACG2450.015.47295718
ACTGTTC5650.014.9680668
TTTGGAC456.5766915E-414.8301273
CACTGTT5600.014.5948037
TAGGACC6100.014.3733374
TAGACCT400.005165334314.2952215
GTACGTG400.005165334314.2952216
GGAAACG400.0053573114.2168217
TACTAGA551.8961958E-413.8701922
GATATAC6100.013.7473981
ACTATAC500.001458641513.3471133
GCTATAC500.001458641513.3471133
TCGTTGG500.001517886813.27679912
CCAACGT802.0526768E-613.02922619
CCGGTTT1102.8067006E-912.93194712
CTGTAGG16500.012.879191
TGTAGGA16800.012.8278642
TATACAC6950.012.7572613
ATACACT6650.012.7546324
Produced by FastQC (version 0.11.5)