FastQCFastQC Report
Thu 26 May 2016
SRR1926509_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926509_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475295
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC13200.27772225670373135No Hit
CCCATGTACTCTGCGTTGATACCAC11460.24111341377460313No Hit
GTCCTACAGTGGACATTTCTAAATT11020.2318560052178121No Hit
CTGTAGGACGTGGAATATGGCAAGA7790.1638982105850051No Hit
GAGTACATGGGAAGCAGTGGTATCA6740.1418066674381174No Hit
GTCCTACAGTGTGCATTTCTCATTT6520.13717796315972186No Hit
GTCCTAAAGTGTGTATTTCTCATTT6500.1367571718616859No Hit
CTTTAGGACGTGAAATATGGCGAGG5010.10540822015800712No Hit
CATGTACTCTGCGTTGATACCACTG4950.10414584626389925No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGGA200.00917806826.6415251
ACTTTCG250.005183723319.5002028
CTAGAAT300.007650478718.2282353
TGTAGGA6650.017.242752
CTGTAGG6450.017.1414471
GATCTAC704.4674744E-617.1266961
TAGGACC2600.016.4499934
CTAGGAC606.7365127E-616.405413
GTCCATG500.002450831215.9849171
TCCTAGA706.944099E-515.6005832
GTCCTAG604.5966858E-415.5408911
GCCCTGA708.55712E-515.2237291
CTACACT1207.2759576E-1215.1477014
TCAGGAC450.004519463514.582593
GTAGGAC7500.014.5825883
TCTAGGA751.250439E-414.5605442
GTCTAGA550.004622705314.5317421
TCTACAC1301.8189894E-1114.3021553
CTAGAGT601.4980182E-414.25665954
AGGACCT4250.013.9542825
Produced by FastQC (version 0.11.5)