FastQCFastQC Report
Thu 26 May 2016
SRR1926509_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926509_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475295
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC33750.7100853154356768No Hit
GTACATGGGAAGCAGTGGTATCAAC31820.669478955175207No Hit
CTGTAGGACGTGGAATATGGCAAGA23960.504107975047076No Hit
GTCCTACAGTGGACATTTCTAAATT22950.48285801449626026No Hit
GAGTACATGGGAAGCAGTGGTATCA15630.32884839941510013No Hit
GTCCTAAAGTGTGTATTTCTCATTT14230.2993930085525831No Hit
GTCCTACAGTGTGCATTTCTCATTT14130.29728905206240336No Hit
CATGTACTCTGCGTTGATACCACTG13930.2930811390820438No Hit
CTTTAGGACGTGAAATATGGCGAGG13860.29160836953891794No Hit
GTATCAACGCAGAGTACTTTTTTTT12450.261942583027383No Hit
CTGTAGGACCTGGAATATGGCGAGA11910.25058121798041216No Hit
GATATACACTGTTCTACAAATCCCG11520.24237578766871098No Hit
ACGCAGAGTACATGGGAAGCAGTGG10380.21839068368066147No Hit
GCGTTGATACCACTGCTTCCCATGT10190.2143931663493199No Hit
GTATCAACGCAGAGTACATGGGAAG9060.19061845801028834No Hit
CTGAAGGACCTGGAATATGGCGAGA8480.1784155103672456No Hit
GGTATCAACGCAGAGTACTTTTTTT8050.16936849745947255No Hit
ACTCTGCGTTGATACCACTGCTTCC7760.16326702363795115No Hit
GCTTCCCATGTACTCTGCGTTGATA7300.15358882378312416No Hit
GTGTATATCAATGAGTTACAATGAA7240.1523264498890163No Hit
GTGTATATCAATGAGTTACAATGAG6970.1466457673655309No Hit
GGTATCAACGCAGAGTACATGGGAA6710.14117548049106346No Hit
TATCAACGCAGAGTACTTTTTTTTT6390.13444281972248814No Hit
GTTCTACAGTGTGGTTTTTATCATT6260.13170767628525443No Hit
TATCAACGCAGAGTACATGGGAAGC6140.1291829284970387No Hit
GATATACACTGTTCTACAATGCCGG5980.12581659811275103No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5960.12539580681471507No Hit
ACCTGGAATATGGCGAGAAAACTGA5700.11992551994024762No Hit
GAATATGGCAAGAAAACTGAAAATC5450.11466562871479818No Hit
GTCCTTCAGTGTGCATTTCTCATTT5130.10793296794622287No Hit
CTGTAGGACATGGAATATGGCAAGA5100.10730178099916893No Hit
ACAGTGGACATTTCTAAATTTTCCA5010.10540822015800712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTACC601.3798399E-615.9209132
TCCAACG1200.015.77853918
TAGGACC4150.014.9570594
AACAGAT704.4218177E-714.9271617
CGGTTTC704.5558227E-714.88787213
ATTCCCG456.891263E-414.73748911
GCCGGTT759.933137E-713.89534711
CCAACGA903.7860445E-813.67473219
CCAACGT500.00153649213.25397119
TCCTAGA801.8713945E-613.1347532
TGTAGGA12700.012.7116582
TGCCGGT751.51376935E-512.63346410
CTGTAGG12500.012.6013371
GATATAC4250.012.5788751
AGGACCT7450.012.5617675
GGACCTG7050.012.4591246
GTAGGAC12350.012.3731433
CCGGTTT854.048743E-612.260612
TAGAAAT1251.2878445E-912.2233684
GTCCTAC11250.012.1346211
Produced by FastQC (version 0.11.5)