FastQCFastQC Report
Thu 26 May 2016
SRR1926508_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926508_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380913
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9870.259114285939309No Hit
CTGTAGGACGTGGAATATGGCAAGA6930.1819313071488765No Hit
GTACATGGGAAGCAGTGGTATCAAC6570.1724803301541297No Hit
CCCATGTACTCTGCGTTGATACCAC6220.16329188029812583No Hit
GTCCTAAAGTGTGTATTTCTCATTT5210.1367766392850861No Hit
GTCCTACAGTGTGCATTTCTCATTT4760.12496291804165256No Hit
CTTTAGGACGTGAAATATGGCGAGG4330.11367425107570496No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGGG405.5866316E-419.9251251
TGTAATC350.00528126219.4328772
TTAAGAC358.726799E-418.6494623
GTCCTAT501.1659088E-418.5967851
TGTAGGA5400.017.129722
CTGTAGG5400.016.7272661
GCCATAT1600.016.604271
TGTAGAA1500.016.3236182
ATAATCT400.002419218216.0160884
CCTACAC556.150762E-515.8237863
GATATAC1700.015.6275491
AGACTCC400.00294803415.5485496
TTAGTTA350.002984439715.52092312
GAACAGT951.4133548E-915.2757686
TAGGACC2050.015.1046044
AGGACGG559.279665E-515.0800645
TGGAGTG507.7210466E-414.5145645
TTTAGGA3000.014.5098822
ATACACT2000.014.414484
TCCTAGA852.3034547E-514.4031912
Produced by FastQC (version 0.11.5)