FastQCFastQC Report
Thu 26 May 2016
SRR1926508_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926508_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380913
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT19730.517966044739875No Hit
CTGTAGGACGTGGAATATGGCAAGA19610.5148157190749594No Hit
GTACATGGGAAGCAGTGGTATCAAC18230.47858697392842986No Hit
CCCATGTACTCTGCGTTGATACCAC17750.4659856712687674No Hit
CTTTAGGACGTGAAATATGGCGAGG12420.3260587063187657No Hit
GTCCTAAAGTGTGTATTTCTCATTT11620.3050565352193283No Hit
GTATCAACGCAGAGTACTTTTTTTT11430.3000685195832119No Hit
GTCCTACAGTGTGCATTTCTCATTT11090.291142596865951No Hit
GATATACACTGTTCTACAAATCCCG10410.2732907514314292No Hit
CTGTAGGACCTGGAATATGGCGAGA9510.24966330894456212No Hit
CTGAAGGACCTGGAATATGGCGAGA9030.2370620062848997No Hit
GAGTACATGGGAAGCAGTGGTATCA8660.22734850215140992No Hit
GTGTATATCAATGAGTTACAATGAA7750.20345853252579987No Hit
GGTATCAACGCAGAGTACTTTTTTT7630.20030820686088424No Hit
GTTCTACAGTGTGGTTTTTATCATT7250.19033217558865148No Hit
GTGTATATCAATGAGTTACAATGAG6530.17143022159915783No Hit
CATGTACTCTGCGTTGATACCACTG6440.16906747735047112No Hit
TATCAACGCAGAGTACTTTTTTTTT6030.15830386466200944No Hit
GATATACACTGTTCTACAATGCCGG5950.15620364755206567No Hit
ACGCAGAGTACATGGGAAGCAGTGG5650.14832783338977668No Hit
CTGTAGAACATATTAGATGAGTGAG5620.14754025197354778No Hit
GCGTTGATACCACTGCTTCCCATGT5360.1407145463662306No Hit
ACCTGGAATATGGCGAGAAAACTGA5190.13625158500760018No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5130.13467642217514236No Hit
GTATCAACGCAGAGTACATGGGAAG5000.13126356937148376No Hit
TTGTAGAACAGTGTATATCAATGAG4930.129425879400283No Hit
GTCCTTCAGTGTGCATTTCTCATTT4860.12758818942908223No Hit
ACAGTGGACATTTCTAAATTTTCCA4300.11288666965947605No Hit
GAATATGGCAAGAAAACTGAAAATC4250.11157403396576122No Hit
GTTCTACAAATCCCGTTTCCAACGA4240.11131150682701825No Hit
CTGTAGGACATGGAATATGGCAAGA4230.11104897968827528No Hit
ATTCCAGGTCCTTCAGTGTGCATTT4200.11026139827204637No Hit
GACCTGGAATATGGCGAGAAAACTG3830.10054789413855658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA907.2759576E-1216.85236719
TAGGACC4250.015.2411574
ATGTCGA508.8483175E-515.1671317
TCCAACG1500.014.53516718
ATTCCCG551.9791983E-413.79735711
TATGTCG551.9902557E-413.788300516
CTAGGAC903.4782715E-813.7611913
TGTAGGA11300.013.6581472
GCCGGTT707.3416304E-613.55097411
CCTAATA500.001464271713.337772
GTAGGAC11000.013.2511623
CTGTAGG11550.013.1940651
CCTACAC655.2886884E-513.1912013
TCTAGGA655.2886884E-513.1912012
AGGACCT7450.013.1697915
GTAGGAA801.925746E-613.0995963
CTCTAGG801.931463E-613.0961411
TAGGACA2500.012.9549834
GATATAC4200.012.9260611
GTATAAA603.9917947E-412.6992881
Produced by FastQC (version 0.11.5)