FastQCFastQC Report
Thu 26 May 2016
SRR1926507_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926507_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462558
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC21140.45702376783019644No Hit
GTACATGGGAAGCAGTGGTATCAAC21000.4539971203611223No Hit
GAGTACATGGGAAGCAGTGGTATCA10500.22699856018056114No Hit
GTCCTACAGTGGACATTTCTAAATT9140.1975968419095551No Hit
GCGTTGATACCACTGCTTCCCATGT7460.16127707228066535No Hit
CATGTACTCTGCGTTGATACCACTG7400.15997993765106214No Hit
CTGTAGGACGTGGAATATGGCAAGA6580.142252431046485No Hit
ACGCAGAGTACATGGGAAGCAGTGG6420.13879340536754312No Hit
GTATCAACGCAGAGTACATGGGAAG5980.1292810847504529No Hit
GTCCTAAAGTGTGTATTTCTCATTT5610.12128208786789982No Hit
ACTCTGCGTTGATACCACTGCTTCC5160.11155357814587577No Hit
GTCCTACAGTGTGCATTTCTCATTT5060.10939168709653709No Hit
GNACATGGGAAGCAGTGGTATCAAC4800.10377077036825652No Hit
GCTTCCCATGTACTCTGCGTTGATA4750.10268982484358717No Hit
CNCATGTACTCTGCGTTGATACCAC4680.1011765011090501No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGCT250.002986714221.4469574
TGCTGGA450.001015325718.2333972
CTGTAGG5000.017.0811961
TGTAGGA5050.017.0599612
TTGTAGA1103.1286618E-1016.9841441
GTATAAA552.3362615E-416.9841441
CAGTCTA350.002687451215.7679669
CCAACGA653.8908183E-815.70428419
TGGACTG400.002864166215.617325
TAGGACC1650.015.5977884
GTATTGG604.5337545E-415.5687971
TCCAACG904.3655746E-1115.13365418
AGGACCT3100.015.1135365
GGACCTG3100.015.1135366
TGTAGAA2950.014.8339522
AAGGACC1450.014.7910044
GTGTAGC550.004567456414.55783751
GTAGGAC5800.014.5227123
CGTGCAA458.616215E-414.307979
TAGCCCT901.226399E-714.2979724
Produced by FastQC (version 0.11.5)