##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926507_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 462558 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.50960744382326 26.0 2.0 30.0 2.0 31.0 2 15.277413859451139 10.0 2.0 30.0 2.0 31.0 3 18.450851568884335 16.0 2.0 30.0 2.0 31.0 4 20.20370202223289 25.0 2.0 33.0 2.0 35.0 5 21.050136415325213 28.0 2.0 35.0 2.0 35.0 6 21.59016598999477 29.0 2.0 35.0 2.0 37.0 7 21.331735695847872 28.0 2.0 35.0 2.0 37.0 8 21.460400641649265 28.0 2.0 35.0 2.0 37.0 9 23.889069046476333 30.0 2.0 37.0 2.0 39.0 10 24.475531717103586 31.0 2.0 37.0 2.0 39.0 11 21.86430026072406 28.0 2.0 35.0 2.0 39.0 12 22.855289498830416 30.0 2.0 35.0 2.0 39.0 13 19.80262150908643 24.0 2.0 35.0 2.0 39.0 14 22.805801650820005 28.0 2.0 36.0 2.0 40.0 15 23.819032424041957 30.0 2.0 37.0 2.0 40.0 16 24.14508234643007 30.0 2.0 38.0 2.0 40.0 17 23.498521266522253 30.0 2.0 37.0 2.0 40.0 18 23.54227794136087 30.0 2.0 37.0 2.0 40.0 19 23.858577735116462 30.0 2.0 37.0 2.0 40.0 20 24.107203853354605 30.0 2.0 38.0 2.0 40.0 21 22.076762265488867 29.0 2.0 36.0 2.0 40.0 22 22.938941711093527 30.0 2.0 37.0 2.0 40.0 23 22.896281115016926 30.0 2.0 37.0 2.0 40.0 24 22.079659199494984 30.0 2.0 37.0 2.0 40.0 25 21.86466778220245 30.0 2.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.575813991215799 1101 2 9.933326789371975 1101 3 6.649613155207753 1101 4 7.876184646146637 1101 5 8.06461905404419 1101 6 7.59566987693465 1101 7 7.24399882568617 1101 8 5.550204412948339 1101 9 5.8190621079295575 1101 10 5.6013520135782535 1101 11 8.150884981603063 1101 12 5.835935835096588 1101 13 9.949172554529717 1101 14 7.6406307869664225 1101 15 7.031694972941175 1101 16 6.447439419320755 1101 17 2.469899048017634 1101 18 4.908343646053609 1101 19 5.613411490890325 1101 20 3.7998073093970532 1101 21 0.8021063054851751 1101 22 4.986295152885351 1101 23 5.978819606602215 1101 24 6.952111848936585 1101 25 7.503672475366788 1102 1 5.172565666569486 1102 2 9.474335724591633 1102 3 6.411027277818821 1102 4 7.445547391244677 1102 5 7.32804670358502 1102 6 6.3372248533556 1102 7 6.364670828664579 1102 8 6.314001260801405 1102 9 5.622974369036534 1102 10 4.994967054531344 1102 11 7.590913524665858 1102 12 4.809307019013193 1102 13 9.101992733234109 1102 14 6.8590441649758525 1102 15 4.916421331839164 1102 16 5.510215542428224 1102 17 6.266939256506092 1102 18 6.39712808387937 1102 19 5.036115635839444 1102 20 5.420212496788466 1102 21 3.757107546487905 1102 22 5.215979938191879 1102 23 5.669294910647885 1102 24 6.858713983461282 1102 25 6.936959271097738 1103 1 3.4822053939307303 1103 2 7.907336506751992 1103 3 4.987225659225754 1103 4 6.558662145343035 1103 5 5.905364053178776 1103 6 5.544261104093863 1103 7 5.636695027748331 1103 8 4.931433859329665 1103 9 4.2840483230965845 1103 10 3.630791595211665 1103 11 4.461745328263795 1103 12 3.2528605810353177 1103 13 6.799485031348944 1103 14 5.298675751484385 1103 15 4.70006619911134 1103 16 4.262583572820741 1103 17 2.19539233486579 1103 18 1.0978001032897176 1103 19 3.1265812606897967 1103 20 3.556222201961898 1103 21 5.1718983584980585 1103 22 1.2652927544480441 1103 23 3.31992622579466 1103 24 4.332845226777003 1103 25 0.5452294197082068 1104 1 5.8187204603728055 1104 2 10.237445152045513 1104 3 6.973724003926801 1104 4 7.310267585891879 1104 5 7.491876818463826 1104 6 7.219524893393789 1104 7 6.864624630752722 1104 8 6.821288211431234 1104 9 5.76466644455472 1104 10 4.994027696990305 1104 11 8.109254095672046 1104 12 5.094228020583923 1104 13 8.906763437598272 1104 14 6.708229541796822 1104 15 6.312922609981392 1104 16 6.027284130928024 1104 17 6.3279389793186205 1104 18 3.9231837061652435 1104 19 4.764280962766669 1104 20 4.157999308824564 1104 21 2.8490506306605 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2204 7 3.5118127002854393 2204 8 3.298950964801019 2204 9 2.1830792927502536 2204 10 1.3662349629557262 2204 11 2.967098729459618 2204 12 3.1858435738574933 2204 13 0.7449536996874002 2204 14 1.8908870681861352 2204 15 2.243658180277343 2204 16 2.5319190286549826 2204 17 -1.9304681330753368 2204 18 1.1797081804605263 2204 19 1.6703618375689224 2204 20 2.2060782778604846 2204 21 3.1327183982310167 2204 22 2.6248920365057202 2204 23 1.6173512550030225 2204 24 3.0112335964949253 2204 25 3.1869135474610246 2205 1 4.945968137211548 2205 2 9.10276335918062 2205 3 5.922678947789844 2205 4 6.446752210521783 2205 5 6.4398966349560745 2205 6 6.362602312559503 2205 7 6.058890746309842 2205 8 5.911576371370259 2205 9 4.908623293849711 2205 10 4.475430681189877 2205 11 6.36150983395364 2205 12 5.614736331198646 2205 13 8.364826346166854 2205 14 6.4108971465222915 2205 15 5.469921408093548 2205 16 5.201827560119241 2205 17 5.609321443481843 2205 18 5.58360055604652 2205 19 4.3544166881499535 2205 20 4.533557014098214 2205 21 -1.0974448402743349 2205 22 2.9590331637523484 2205 23 4.0685018650477645 2205 24 5.918329661362762 2205 25 6.045926786729886 2206 1 3.267654884199498 2206 2 7.414811551951708 2206 3 5.211835574295868 2206 4 1.8648244996784094 2206 5 5.165197839775352 2206 6 5.197542071595649 2206 7 4.943228095707433 2206 8 5.117600467755803 2206 9 3.7471775107171794 2206 10 3.115189717334456 2206 11 3.006088147206654 2206 12 3.7520857287890053 2206 13 6.656392611227094 2206 14 -0.35898237154999535 2206 15 1.6289575526718636 2206 16 2.647610692649362 2206 17 3.5948636121565407 2206 18 -0.9525440222667321 2206 19 0.34236849537886727 2206 20 1.7419907490379707 2206 21 2.4543623886413286 2206 22 -2.446990932633195 2206 23 -3.015835484349825 2206 24 0.1303778541338474 2206 25 1.751950883115427 2207 1 1.3638768089354798 2207 2 -12.735790857686847 2207 3 -5.684237607319481 2207 4 -3.887785942088657 2207 5 1.2125130264479687 2207 6 1.1724491071452547 2207 7 2.759857576593941 2207 8 2.951647768068163 2207 9 0.5261154848358771 2207 10 0.5189082953502222 2207 11 -1.4130402181064525 2207 12 -2.240997716101667 2207 13 0.10579020158854036 2207 14 -4.934142270404674 2207 15 -1.1709829500798854 2207 16 -0.2496375596189715 2207 17 0.9201350683516942 2207 18 0.8673578072736525 2207 19 0.34919580461581745 2207 20 0.16964209516321205 2207 21 -3.541829787470526 2207 22 -3.877394026490105 2207 23 -1.0817286867892122 2207 24 0.7219738678182139 2207 25 1.125504356267296 2208 1 4.90477684876025 2208 2 6.638163742074205 2208 3 6.4434804333017865 2208 4 8.107793508329504 2208 5 3.3683473554005765 2208 6 5.780653009972813 2208 7 6.4967353145469495 2208 8 6.553081459824437 2208 9 5.364466739316164 2208 10 4.732448717492797 2208 11 6.478516930439945 2208 12 6.434032152476586 2208 13 8.474846354515659 2208 14 3.625077164083578 2208 15 5.008507292851217 2208 16 5.486523332455899 2208 17 6.200581105786977 2208 18 1.2245860032115239 2208 19 3.4342615154879788 2208 20 4.534596296676554 2208 21 7.105043968005379 2208 22 6.268922182191698 2208 23 2.4389801941347216 2208 24 3.7752195866553286 2208 25 5.120884682830418 2209 1 2.8133344418739377 2209 2 4.25535958479103 2209 3 4.807540264104407 2209 4 -0.25875096526607066 2209 5 4.4712463067151695 2209 6 4.805905741191857 2209 7 5.211579884658434 2209 8 3.686788471350461 2209 9 3.87970915435875 2209 10 3.1214773203381263 2209 11 -0.6642193787251394 2209 12 2.6815101267601555 2209 13 2.038407142170083 2209 14 3.0510491578545995 2209 15 3.3776225032453304 2209 16 2.912081470831623 2209 17 0.8405858183009691 2209 18 2.9679805539140958 2209 19 3.3984162007388683 2209 20 3.277294543228642 2209 21 5.35842617856715 2209 22 4.206331840126619 2209 23 0.1597924382127367 2209 24 -2.7927878808668396 2209 25 0.059318406305532534 2210 1 -2.430261449570594 2210 2 -12.735790857686847 2210 3 -7.054627625985361 2210 4 -2.3547178607447137 2210 5 -0.883825841155268 2210 6 -1.5237260246620608 2210 7 -1.4477725114959163 2210 8 -18.8113151948948 2210 9 -12.091587629418962 2210 10 -7.090585106097201 2210 11 -9.56138812392562 2210 12 -4.740899474624115 2210 13 -5.106676641912786 2210 14 -3.8326457252299697 2210 15 -5.368942802062726 2210 16 -4.945400077220569 2210 17 -4.452105164808081 2210 18 -4.677931513877738 2210 19 -9.801909412006642 2210 20 -7.029745532960046 2210 21 -5.293488431083194 2210 22 -9.63317226272811 2210 23 -8.016922058764195 2210 24 -10.687483511754108 2210 25 -8.78890111905772 2211 1 0.4059762107427858 2211 2 4.712662750560575 2211 3 1.038624171985596 2211 4 2.0581762572240585 2211 5 1.2638812331426337 2211 6 0.030301977197435548 2211 7 -18.730265880196182 2211 8 -11.973686328915418 2211 9 -8.011150647275615 2211 10 -6.630000321170076 2211 11 -8.668955325653862 2211 12 -4.382416196437674 2211 13 -6.0485912417104295 2211 14 -4.418356983399455 2211 15 -4.823709817841117 2211 16 -10.771051585328529 2211 17 -6.849764384365628 2211 18 -5.214495953703821 2211 19 -7.876405569954629 2211 20 -6.100826913364216 2211 21 -3.7854214891378284 2211 22 -5.262315115964459 2211 23 -7.272418517513408 2211 24 -19.47926070008302 2211 25 -19.276964779535174 2212 1 -1.0881766050226958 2212 2 -12.735790857686847 2212 3 -7.230193767561355 2212 4 -17.654452608755324 2212 5 -18.401067220465613 2212 6 -18.93619286816339 2212 7 -9.013259077475093 2212 8 -8.375941045234935 2212 9 -4.5841641825866475 2212 10 -4.976229018834712 2212 11 -4.943022581422149 2212 12 -2.525653800757752 2212 13 -5.0608764650781275 2212 14 -3.410584699228057 2212 15 -3.2870575280592256 2212 16 -3.490025566673644 2212 17 -3.6969977106858494 2212 18 -2.6585681186522763 2212 19 -3.030396032118876 2212 20 -3.119045231619353 2212 21 -1.2202626420938572 2212 22 -2.3285908014964036 2212 23 -2.3539635066430797 2212 24 -1.5051110402190737 2212 25 -1.9368287251134042 2213 1 -16.97933306760773 2213 2 -12.735790857686847 2213 3 -8.450648846144514 2213 4 -6.242038815651876 2213 5 -18.401067220465613 2213 6 -10.482399764715112 2213 7 -18.730265880196182 2213 8 -10.208556574205144 2213 9 -8.39084492384867 2213 10 -7.676102347476512 2213 11 -6.501971678302279 2213 12 -5.69320716693181 2213 13 -17.19420979841146 2213 14 -11.569834054142435 2213 15 -7.238969327261664 2213 16 -6.823490262896961 2213 17 -5.403457949953967 2213 18 -5.322016394514343 2213 19 -6.088965114924413 2213 20 -5.9561646311032845 2213 21 -4.115716547794069 2213 22 -4.7260104590145815 2213 23 -4.62578147520982 2213 24 -3.7413296656002615 2213 25 -4.26730960712138 2214 1 -16.97933306760773 2214 2 -12.735790857686847 2214 3 -11.8206573604059 2214 4 -17.654452608755324 2214 5 -18.401067220465613 2214 6 -18.93619286816339 2214 7 -15.458660941924578 2214 8 -14.107611491191097 2214 9 -13.576045008991283 2214 10 -13.577388000946073 2214 11 -13.828780958847192 2214 12 -12.270318703755988 2214 13 -17.19420979841146 2214 14 -15.624574369170107 2214 15 -21.273452085882354 2214 16 -16.973402991717734 2214 17 -20.978630363747072 2214 18 -16.424308859393015 2214 19 -14.38105110896443 2214 20 -13.39795049742938 2214 21 -10.999545836853242 2214 22 -11.823073038835782 2214 23 -11.702099057159584 2214 24 -10.777100206255861 2214 25 -10.513075890646284 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 110680.0 3 11.0 4 479.0 5 1937.0 6 2771.0 7 2597.0 8 2028.0 9 1991.0 10 1841.0 11 1739.0 12 1625.0 13 1686.0 14 2455.0 15 4683.0 16 6619.0 17 7106.0 18 7468.0 19 8856.0 20 10296.0 21 12116.0 22 12454.0 23 13462.0 24 13758.0 25 13969.0 26 11768.0 27 9814.0 28 10697.0 29 12083.0 30 11836.0 31 13468.0 32 16820.0 33 20003.0 34 23438.0 35 25922.0 36 24601.0 37 24256.0 38 15225.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.09518761924952 18.576363341605457 12.811732282482636 25.516716756662383 2 11.7048147368564 20.456350957719128 44.589786592611205 23.24904771281327 3 20.791185219475754 26.033898771192458 27.82142186003236 25.353494149299426 4 12.100424611440474 17.814152379426375 37.27442517224804 32.810997836885115 5 11.544727617479387 38.40574828786348 37.188977458428454 12.860546636228683 6 30.449876126211795 37.875963920649774 18.226056350528587 13.448103602609846 7 26.05324471945304 31.514266070814713 21.53135861884573 20.90113059088652 8 24.895259713873358 39.92765096846816 20.13699141993732 15.04009789772117 9 26.555547861013483 16.538624677550594 19.744940357680786 37.16088710375513 10 15.227326028682336 31.403935218164165 33.35313289745068 20.015605855702823 11 32.41696668584039 23.15399405538075 24.04477027048705 20.384268988291804 12 25.104553556885623 25.864129699174814 31.494766013383774 17.536550730555792 13 29.317375703709814 21.765512548202835 24.04316086857897 24.87395087950838 14 19.672688955721906 25.608851228239633 26.943121199481922 27.77533861655654 15 24.108152381969855 32.5777000258184 22.554597010407484 20.759550581804262 16 19.04140462149691 28.842933243065083 32.31484615795756 19.800815977480447 17 20.95508242675032 29.580190883000736 30.437384591425836 19.027342098823112 18 20.604026845637584 27.408869588103695 33.6719743825942 18.315129183664517 19 22.768761343609437 27.492086873416277 27.671392622598663 22.067759160375623 20 22.809687651084992 29.401817247591854 28.455686432648886 19.332808668674268 21 20.10217806562454 27.313643710510547 27.47843974998022 25.105738473884685 22 21.057045322327475 30.668144847041262 27.025893768591597 21.24891606203967 23 19.713384204535124 30.143784003704972 29.010218965465008 21.1326128262949 24 21.99833428641257 29.785426667077953 28.779591923899194 19.43664712261028 25 21.521603629028768 28.089479106247783 28.478829222684894 21.91008804203856 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 837.0 1 837.0 2 1443.0 3 2049.0 4 2049.0 5 2049.0 6 2696.5 7 3344.0 8 3344.0 9 3344.0 10 3789.5 11 4235.0 12 4235.0 13 4235.0 14 5180.0 15 6125.0 16 6125.0 17 6125.0 18 8399.5 19 10674.0 20 10674.0 21 10674.0 22 14888.5 23 19103.0 24 19103.0 25 19103.0 26 25312.0 27 31521.0 28 31521.0 29 31521.0 30 38663.0 31 45805.0 32 45805.0 33 45805.0 34 49285.0 35 52765.0 36 52765.0 37 52765.0 38 53746.5 39 54728.0 40 54728.0 41 54728.0 42 56010.5 43 57293.0 44 57293.0 45 57293.0 46 60340.5 47 63388.0 48 63388.0 49 63388.0 50 55550.5 51 47713.0 52 47713.0 53 47713.0 54 37772.5 55 27832.0 56 27832.0 57 27832.0 58 22303.5 59 16775.0 60 16775.0 61 16775.0 62 13237.0 63 9699.0 64 9699.0 65 9699.0 66 7323.0 67 4947.0 68 4947.0 69 4947.0 70 3610.5 71 2274.0 72 2274.0 73 2274.0 74 1606.0 75 938.0 76 938.0 77 938.0 78 643.0 79 348.0 80 348.0 81 348.0 82 237.0 83 126.0 84 126.0 85 126.0 86 76.5 87 27.0 88 27.0 89 27.0 90 18.0 91 9.0 92 9.0 93 9.0 94 5.0 95 1.0 96 1.0 97 1.0 98 1.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.320059322290396 2 36.32063438530952 3 5.664154549267335 4 13.648882950894805 5 13.348163905931798 6 14.74517790201445 7 9.439248699622533 8 7.42739288910796 9 0.10160887931891785 10 1.3660989540771102 11 5.663505981952534 12 5.762952970222113 13 16.130733875535608 14 1.5193770294752225 15 2.8679646660526896 16 2.924173833335495 17 2.825159223275784 18 1.4307394964523368 19 1.3736655727497957 20 0.2834239165683006 21 4.365506595929592 22 1.5226198660492307 23 0.10290601394852106 24 8.88904742756584 25 0.10939168709653709 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 462558.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.2873961544646 #Duplication Level Percentage of deduplicated Percentage of total 1 96.86968451749371 82.61763158801492 2 1.8614610229550452 3.175183273817237 3 0.4482505443172049 1.1469036524890754 4 0.22100606456818017 0.7539612712546225 5 0.13206029415700313 0.5631539312021726 6 0.07759742211305101 0.3970849248192596 7 0.06278488850497667 0.3748331760906485 8 0.039391596869065494 0.26876853818631646 9 0.03236476107251478 0.24842755791325463 >10 0.21461177545283092 3.4899781098185785 >50 0.018394188438525628 1.1090680315442976 >100 0.019193934906734843 3.321065231299316 >500 0.002399241863341848 1.3371112908380398 >1k 7.99747287780616E-4 1.1968294227123688 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 2114 0.45702376783019644 No Hit GTACATGGGAAGCAGTGGTATCAAC 2100 0.4539971203611223 No Hit GAGTACATGGGAAGCAGTGGTATCA 1050 0.22699856018056114 No Hit GTCCTACAGTGGACATTTCTAAATT 914 0.1975968419095551 No Hit GCGTTGATACCACTGCTTCCCATGT 746 0.16127707228066535 No Hit CATGTACTCTGCGTTGATACCACTG 740 0.15997993765106214 No Hit CTGTAGGACGTGGAATATGGCAAGA 658 0.142252431046485 No Hit ACGCAGAGTACATGGGAAGCAGTGG 642 0.13879340536754312 No Hit GTATCAACGCAGAGTACATGGGAAG 598 0.1292810847504529 No Hit GTCCTAAAGTGTGTATTTCTCATTT 561 0.12128208786789982 No Hit ACTCTGCGTTGATACCACTGCTTCC 516 0.11155357814587577 No Hit GTCCTACAGTGTGCATTTCTCATTT 506 0.10939168709653709 No Hit GNACATGGGAAGCAGTGGTATCAAC 480 0.10377077036825652 No Hit GCTTCCCATGTACTCTGCGTTGATA 475 0.10268982484358717 No Hit CNCATGTACTCTGCGTTGATACCAC 468 0.1011765011090501 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 2.1618910493386776E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAAGCT 25 0.0029867142 21.446957 4 TGCTGGA 45 0.0010153257 18.233397 2 CTGTAGG 500 0.0 17.081196 1 TGTAGGA 505 0.0 17.059961 2 TTGTAGA 110 3.1286618E-10 16.984144 1 GTATAAA 55 2.3362615E-4 16.984144 1 CAGTCTA 35 0.0026874512 15.767966 9 CCAACGA 65 3.8908183E-8 15.704284 19 TGGACTG 40 0.0028641662 15.61732 5 TAGGACC 165 0.0 15.597788 4 GTATTGG 60 4.5337545E-4 15.568797 1 TCCAACG 90 4.3655746E-11 15.133654 18 AGGACCT 310 0.0 15.113536 5 GGACCTG 310 0.0 15.113536 6 TGTAGAA 295 0.0 14.833952 2 AAGGACC 145 0.0 14.791004 4 GTGTAGC 55 0.0045674564 14.5578375 1 GTAGGAC 580 0.0 14.522712 3 CGTGCAA 45 8.616215E-4 14.30797 9 TAGCCCT 90 1.226399E-7 14.297972 4 >>END_MODULE