FastQCFastQC Report
Thu 26 May 2016
SRR1926507_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926507_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462558
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC64901.4030672910208017No Hit
GTACATGGGAAGCAGTGGTATCAAC57651.2463301899437476No Hit
GAGTACATGGGAAGCAGTGGTATCA28390.6137608689072506No Hit
CATGTACTCTGCGTTGATACCACTG24490.5294471179830421No Hit
CTGTAGGACGTGGAATATGGCAAGA20910.4520514184167174No Hit
GTCCTACAGTGGACATTTCTAAATT20330.43951245033055314No Hit
GCGTTGATACCACTGCTTCCCATGT19600.4237306456703808No Hit
ACGCAGAGTACATGGGAAGCAGTGG18720.4047060044362004No Hit
GTATCAACGCAGAGTACATGGGAAG16210.35044253909779965No Hit
ACTCTGCGTTGATACCACTGCTTCC14870.3214731990366614No Hit
CTTTAGGACGTGAAATATGGCGAGG13830.29898953212353907No Hit
GTCCTAAAGTGTGTATTTCTCATTT13180.2849372403028377No Hit
GTATCAACGCAGAGTACTTTTTTTT13000.2810458364140281No Hit
GCTTCCCATGTACTCTGCGTTGATA12780.276289676105483No Hit
GATATACACTGTTCTACAAATCCCG12050.2605078714453106No Hit
GTCCTACAGTGTGCATTTCTCATTT12020.25985930413050906No Hit
TATCAACGCAGAGTACATGGGAAGC10820.23391661153844492No Hit
GGTATCAACGCAGAGTACATGGGAA10790.2332680442236433No Hit
CTGTAGGACCTGGAATATGGCGAGA10050.2172700504585371No Hit
CTGAAGGACCTGGAATATGGCGAGA9050.1956511399651503No Hit
CAGTGGTATCAACGCAGAGTACATG8860.19154354697140683No Hit
GTGTATATCAATGAGTTACAATGAG8320.17986933530497795No Hit
GGTATCAACGCAGAGTACTTTTTTT8270.17878838978030864No Hit
GTTCTACAGTGTGGTTTTTATCATT7580.16387134153987176No Hit
CATGGGAAGCAGTGGTATCAACGCA7520.16257420691026855No Hit
GTGTATATCAATGAGTTACAATGAA7280.15738566839185572No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7010.1515485625586413No Hit
GCAGTGGTATCAACGCAGAGTACAT6610.14290099836128659No Hit
GTGGTATCAACGCAGAGTACATGGG6600.1426848092563527No Hit
ACATGGGAAGCAGTGGTATCAACGC6410.13857721626260922No Hit
GATATACACTGTTCTACAATGCCGG6360.1374962707379399No Hit
TATCAACGCAGAGTACTTTTTTTTT6360.1374962707379399No Hit
CCATGTACTCTGCGTTGATACCACT5930.12820013922578358No Hit
ATACCACTGCTTCCCATGTACTCTG5630.12171446607776755No Hit
TTGTAGAACAGTGTATATCAATGAG5580.1206335205530982No Hit
GGGAAGCAGTGGTATCAACGCAGAG5540.11976876413336272No Hit
CTGCTTCCCATGTACTCTGCGTTGA5520.11933638592349499No Hit
CTGTAGAACATATTAGATGAGTGAG5410.11695830576922246No Hit
GTACTCTGCGTTGATACCACTGCTT5180.1119859563557435No Hit
CCACTGCTTCCCATGTACTCTGCGT5070.10960787620147094No Hit
AAGCAGTGGTATCAACGCAGAGTAC5040.10895930888666935No Hit
GTTCTACAAATCCCGTTTCCAACGA5040.10895930888666935No Hit
CTTCCCATGTACTCTGCGTTGATAC4920.10636503962746294No Hit
GATACCACTGCTTCCCATGTACTCT4830.10441933768305811No Hit
GCAGAGTACATGGGAAGCAGTGGTA4790.10355458126332265No Hit
ACCTGGAATATGGCGAGAAAACTGA4720.10204125752878558No Hit
GTCCTTCAGTGTGCATTTCTCATTT4660.10074412289918237No Hit
ATGGGAAGCAGTGGTATCAACGCAG4650.1005279337942485No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGAAC350.002112663116.3478243
TTAGACC350.002114199816.3460454
TGGCGAA350.00220654316.24179618
CCAGTAC508.414958E-515.2579713
GTCCTAG508.438427E-515.2529921
TAGGACC4150.015.1644054
TCCAACG1850.014.33960418
AGACTGC602.4727562E-514.3027916
GCCTTAG400.00515129514.299681
GACAGGT400.005256311514.2562627
CTCTTAG551.898545E-413.8663551
ATGTCGA707.4408526E-613.5348317
TAGACAG500.001455612413.3492725
TAGGACT1009.495125E-913.3492724
CCAACGA1500.013.26413319
TATACTG655.2508265E-513.2025765
CTATACT801.909717E-613.1108924
TGTAGGA9950.013.0358662
ACTGTTC3600.012.9334338
CTGTAGG9850.012.6785651
Produced by FastQC (version 0.11.5)