FastQCFastQC Report
Thu 26 May 2016
SRR1926506_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926506_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386265
Sequences flagged as poor quality0
Sequence length25
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17770.46004685902165615No Hit
CTGTAGGACGTGGAATATGGCAAGA13620.35260766572171953No Hit
GTCCTAAAGTGTGTATTTCTCATTT13030.33733317799955986No Hit
CTTTAGGACGTGAAATATGGCGAGG10160.2630318563680375No Hit
GTCCTACAGTGTGCATTTCTCATTT10150.262772966745628No Hit
CTGTAGGACCTGGAATATGGCGAGA6450.16698380645411828No Hit
CTGAAGGACCTGGAATATGGCGAGA5250.13591705176498No Hit
GTCCTTCAGTGTGCATTTCTCATTT5230.13539927252016104No Hit
GAATATGGCAAGAAAACTGAAAATC3880.10044917349488047No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGC355.8031797E-520.3684677
GACGGGG355.8031797E-520.3684677
CGTGCAA401.715124E-518.3655369
TTCTAGG601.7528724E-518.3137532
TTAAAGG300.007589518.2519993
GTAGAAC1100.017.9201453
GACCTAC450.001165578317.8605271
AGGACGA350.001179525617.8314695
ACACAGT350.001180934817.8282976
AAGTGTC350.001359105817.4586877
ACGTGCA402.2839582E-417.0359068
ACGGGGA350.001838789616.6882368
CAACACA452.6872032E-416.6759384
TGTAGAA1257.2759576E-1116.4823782
TTTAGAA1700.016.1591952
TAGGACA2400.016.080374
CAATATG851.1823431E-1015.95426813
CTGTAGG10450.015.7668281
CGTGATA350.002715959415.7418889
GTCCTAA5400.015.627961
Produced by FastQC (version 0.11.5)