FastQCFastQC Report
Thu 26 May 2016
SRR1926506_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926506_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386265
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA39471.02183733965024No Hit
GTCCTACAGTGGACATTTCTAAATT38701.0019028387247098No Hit
CTTTAGGACGTGAAATATGGCGAGG30580.7916844653282074No Hit
GTCCTAAAGTGTGTATTTCTCATTT30460.7885777898592935No Hit
GTCCTACAGTGTGCATTTCTCATTT27220.7046975521986201No Hit
CTGTAGGACCTGGAATATGGCGAGA19230.497844743893441No Hit
CTGAAGGACCTGGAATATGGCGAGA16920.4380412411168498No Hit
GTATCAACGCAGAGTACTTTTTTTT13590.3518309968544911No Hit
GTCCTTCAGTGTGCATTTCTCATTT10970.2840019157832058No Hit
GAATATGGCAAGAAAACTGAAAATC10770.2788241233350161No Hit
ACCTGGAATATGGCGAGAAAACTGA9060.23455399790299405No Hit
CTGTAGGACATGGAATATGGCAAGA8770.22704619885311897No Hit
GGTATCAACGCAGAGTACTTTTTTT8510.22031506867047235No Hit
GACCTGGAATATGGCGAGAAAACTG8410.2177261724463775No Hit
GGAATATGGCGAGAAAACTGAAAAT8140.21073615264132137No Hit
CCATATTCCAGGTCCTTCAGTGTGC7930.20529947057072218No Hit
ACAGTGGACATTTCTAAATTTTCCA7900.20452280170349374No Hit
GCCATATTCCACGTCCTACAGTGGA7710.19960389887771346No Hit
ATTCCAGGTCCTTCAGTGTGCATTT7530.19494388567434273No Hit
CCATATTTCACGTCCTAAAGTGTGT7360.1905427620933815No Hit
CCTAAAGTGTGTATTTCTCATTTTC6650.172161598902308No Hit
CCACTGTAGGACGTGGAATATGGCA6450.16698380645411828No Hit
TATCAACGCAGAGTACTTTTTTTTT6430.16646602720929932No Hit
CACTTTAGGACGTGAAATATGGCGA6330.16387713098520446No Hit
CTACAGTGGACATTTCTAAATTTTC5880.1522270979767776No Hit
GAAATATGGCGAGGAAAACTGAAAA5820.15067376024232068No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5630.14575485741654046No Hit
ATACACACTTTAGGACGTGAAATAT5370.1390237272338938No Hit
GTCCACTGTAGGACGTGGAATATGG5330.1379881687442559No Hit
ACGTGGAATATGGCAAGAAAACTGA5140.13306926591847565No Hit
ATTTAGAAATGTCCACTGTAGGACG4710.12193701215486778No Hit
GTAGGACGTGGAATATGGCAAGAAA4400.11391143386017373No Hit
GAAATACACACTTTAGGACGTGAAA4240.10976919990162194No Hit
TTCCAGGTCCTTCAGTGTGCATTTC4190.10847475178957453No Hit
ACGTCCTACAGTGGACATTTCTAAA4140.10718030367752708No Hit
ATTCCACGTCCTACAGTGGACATTT4100.10614474518788913No Hit
TCCTAAAGTGTGTATTTCTCATTTT4070.10536807632066068No Hit
GTCCTACAGTGGACATTTCTAAATA3920.10148473198451839No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTC250.005905886619.0652436
GCATAAT250.00591033219.0627611
GATCTAC250.00591033219.0627611
CGAATAT250.006085855418.96644612
AGTGTAA307.800536E-418.96644610
AGGAACT402.683236E-416.6820875
CTAGGAC601.4066409E-615.8897723
AATCCCG508.877529E-515.16137419
ATAGGAC1203.6379788E-1214.3007953
GGAATGG400.005267914.2506828
ACAGGTA400.005267914.2506828
GACATGA801.2791133E-714.2506827
TAGGACC5500.014.2122734
GGTGAGG551.99679E-413.78306619
TCTACAC706.967668E-613.6198033
CTATAGG1204.9112714E-1113.5027891
CGAAATC1205.2750693E-1113.434566513
ACTGTTC1009.975338E-913.3006368
AGGACAT3600.013.2397525
TAGGACA3750.013.2185694
Produced by FastQC (version 0.11.5)