FastQCFastQC Report
Thu 26 May 2016
SRR1926505_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926505_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526356
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14740.28003860505057415No Hit
CTGTAGGACGTGGAATATGGCAAGA10600.20138461421547396No Hit
GTCCTACAGTGTGCATTTCTCATTT9770.1856158189514321No Hit
GTCCTAAAGTGTGTATTTCTCATTT8540.16224760428303278No Hit
CTGTAGGACCTGGAATATGGCGAGA6230.11836095722286817No Hit
CTTTAGGACGTGAAATATGGCGAGG6160.11703105882710561No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAC352.220725E-422.8449251
ACAGCCC358.189339E-519.4796318
GTACTAG551.0769252E-519.3835721
CCCCTGT501.1392944E-418.6566891
GTGTAGG804.2669853E-818.3235341
TAGTACC300.00862136917.8550244
GATCTAC450.001208213617.7682741
TGTAGGA7900.016.7785322
TAGGACC2750.016.3616964
GTCCTAG901.6369631E-716.2875841
GTACTGA500.002444985815.9914481
CCTTACA603.7870818E-415.9424872
GTCCTAA3400.015.677891
CTGTAGG7450.015.5621461
TAGGACA2650.015.362064
CCTGTAC651.4364279E-515.1474573
ACCAGCA550.003902753614.9072612
CTGTAGA1201.8475475E-814.4367241
GTGTAAC658.413944E-414.3512991
TAAGAGT450.00518648114.284024
Produced by FastQC (version 0.11.5)