FastQCFastQC Report
Thu 26 May 2016
SRR1926505_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926505_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526356
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA33610.6385412154511395No Hit
GTCCTACAGTGGACATTTCTAAATT31580.5999741619740252No Hit
GTCCTACAGTGTGCATTTCTCATTT21310.40485906876714617No Hit
CTTTAGGACGTGAAATATGGCGAGG17920.3404539893152163No Hit
CTGTAGGACCTGGAATATGGCGAGA17620.33475442476194817No Hit
GTCCTAAAGTGTGTATTTCTCATTT17270.32810493278313535No Hit
GTATCAACGCAGAGTACTTTTTTTT13370.2540105935906497No Hit
CTGAAGGACCTGGAATATGGCGAGA10780.20480435294743482No Hit
GGTATCAACGCAGAGTACTTTTTTT9070.17231683499380648No Hit
GATATACACTGTTCTACAAATCCCG8700.16528737204477578No Hit
GTGTATATCAATGAGTTACAATGAA7640.14514891062322838No Hit
ACCTGGAATATGGCGAGAAAACTGA7390.14039927349550493No Hit
CTGTAGGACATGGAATATGGCAAGA7290.13849941864441556No Hit
GTCCTTCAGTGTGCATTTCTCATTT7150.13583962185289045No Hit
GTGTATATCAATGAGTTACAATGAG7000.13298983957625637No Hit
GACCTGGAATATGGCGAGAAAACTG6660.1265303330825525No Hit
GAATATGGCAAGAAAACTGAAAATC6650.12634034759744356No Hit
TATCAACGCAGAGTACTTTTTTTTT6050.11494121849090728No Hit
ACAGTGGACATTTCTAAATTTTCCA5820.11057155233340174No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5620.10677184263122297No Hit
GGAATATGGCGAGAAAACTGAAAAT5470.1039220603545889No Hit
ATTCCAGGTCCTTCAGTGTGCATTT5440.1033521038992621No Hit
GATATACACTGTTCTACAATGCCGG5360.1018322200183906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC1000.017.1683373
TTGCACT350.002113320916.347674
GCCTTAG551.0856731E-515.6045951
TAGGACC6750.015.5403794
TGTCGAG456.890584E-414.73817518
TCTAGGA1250.014.5032552
CTTGCAC400.00513520614.3069483
GATATAC3600.014.3042121
TTAGAAC551.8911593E-413.8734043
GTCCTAT1101.7462298E-1013.8707511
TGTAGGA16300.013.7563152
GGCGAGG4550.013.74329219
CAGGACT706.962029E-613.6230584
AGGACCT9800.013.4284445
TCAGTAC500.001452894813.3531513
CTGTAGG16200.013.3035061
CCAACGA500.001527831813.26435819
GGACCTG9000.013.2446416
GTAGGAC16600.013.042883
GTAGACC1206.89397E-1012.7172863
Produced by FastQC (version 0.11.5)