FastQCFastQC Report
Thu 26 May 2016
SRR1926504_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926504_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490618
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12940.2637489859727935No Hit
CTGTAGGACGTGGAATATGGCAAGA8910.18160768663196214No Hit
GTCCTACAGTGTGCATTTCTCATTT7090.14451161596190926No Hit
GTCCTAAAGTGTGTATTTCTCATTT6740.13737775621766835No Hit
CTGTAGGACCTGGAATATGGCGAGA5510.11230733483076447No Hit
CTTTAGGACGTGAAATATGGCGAGG5260.10721172072773522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG601.0152944E-619.9982511
AGGACCG350.001150364917.9000385
TGTAGGA6600.016.375372
TAGGACC3150.015.6754734
AGACTGT400.002817821215.6559346
GATATAC2400.015.5541951
CTGTAGG6450.015.089121
CTAGAGT651.7181284E-514.8628654
TAGACTG507.322064E-414.6183645
GTCCTAC7600.014.560131
GTCTTAG550.004596935614.5441821
CTCTAGG658.3828164E-414.3577181
GTAGGAC7450.013.9808473
AGGACGG450.00614733113.9222525
TCCTACA8150.013.7646192
CCTAGAG652.3882878E-413.4940643
CCAACGA951.5788828E-913.43605919
TAGGACA1850.013.3453354
GGACGAG550.001509369813.2838236
TATACAC3400.013.2212073
Produced by FastQC (version 0.11.5)