FastQCFastQC Report
Thu 26 May 2016
SRR1926504_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926504_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490618
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA27040.5511416213836426No Hit
GTCCTACAGTGGACATTTCTAAATT26920.5486957266141886No Hit
GTCCTACAGTGTGCATTTCTCATTT17880.3644383206486513No Hit
CTTTAGGACGTGAAATATGGCGAGG17610.35893505741737974No Hit
GTCCTAAAGTGTGTATTTCTCATTT16660.3395717238258686No Hit
CTGTAGGACCTGGAATATGGCGAGA16220.33060344300453715No Hit
GTATCAACGCAGAGTACTTTTTTTT12060.24581242433013056No Hit
GATATACACTGTTCTACAAATCCCG11720.2388823891500108No Hit
GTACATGGGAAGCAGTGGTATCAAC11310.23052558202104287No Hit
CTGAAGGACCTGGAATATGGCGAGA10820.22053817837910553No Hit
CCCATGTACTCTGCGTTGATACCAC9210.18772242355559723No Hit
GTGTATATCAATGAGTTACAATGAA8710.17753119534953873No Hit
GGTATCAACGCAGAGTACTTTTTTT8290.1689705636564496No Hit
GTGTATATCAATGAGTTACAATGAG7810.15918698457863348No Hit
GATATACACTGTTCTACAATGCCGG7290.14858810724433266No Hit
GTTCTACAGTGTGGTTTTTATCATT7000.14267719488481873No Hit
CTGTAGAACATATTAGATGAGTGAG6400.13044772103754856No Hit
ACCTGGAATATGGCGAGAAAACTGA6190.126167405191004No Hit
CTGTAGGACATGGAATATGGCAAGA5910.12046031739561126No Hit
TATCAACGCAGAGTACTTTTTTTTT5840.11903354544676306No Hit
TTGTAGAACAGTGTATATCAATGAG5670.11556852785670317No Hit
GTCCTTCAGTGTGCATTTCTCATTT5620.11454940503609734No Hit
GAATATGGCAAGAAAACTGAAAATC5530.11271498395900681No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5490.11189968570252214No Hit
ACAGTGGACATTTCTAAATTTTCCA5460.11128821201015862No Hit
GAGTACATGGGAAGCAGTGGTATCA5430.11067673831779509No Hit
GACCTGGAATATGGCGAGAAAACTG5310.10823084354834107No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAACG250.00589474819.0727253
CCAACGA1050.018.0478719
TCCAACG1850.016.38941818
GTCTTAG350.00211851616.3413451
GGACCTA551.0861881E-515.6033566
TCTAGGA1600.015.4997692
TAGGACC5700.015.0558714
ACTGTGC1101.0913936E-1114.6837938
AGCAACG400.005133277714.3074792
TAGGCTG602.4728994E-514.3030765
GTTCTAG759.27017E-713.9809281
TGTAGGA13750.013.5964412
CCAACGT707.4369636E-613.53590319
TGTCGAG707.4369636E-613.53590318
CTAGGAC1850.013.4024553
CTGTAGG13250.013.3814041
ATCCCGT1153.8562575E-1013.19220410
GAACAGT1550.012.9189086
AGGACCT9550.012.8802585
GATATAC3950.012.7903791
Produced by FastQC (version 0.11.5)